RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:31293
  • Symbol:N
  • Description:Notch
DataSource: http://genomernai.dkfz.de/v16/genedetails/31293

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 31293 FBgn0004647 N DRSC18685 0.360984741 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 31293 FBgn0004647 N DRSC18685 0.818661042 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00007-A-0 31293 FBgn0004647 DRSC18685 np Excess branching no Neural outgrowth Identification of neural outgrowth genes using genome-wide RNAi. Sepp et al. 2008 18604272 Cell line Primary neural cells Morphology High content (microscopy) DRSC dsRNA Visual inspection np
GR00021-A-0 31293 ND DRSC12648 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC17788 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC21284 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC14928 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC14033 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC22780 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC17494 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC17274 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC20946 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC19611 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC20995 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC20935 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC20764 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC17447 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC17609 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC21332 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC01591 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC19103 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC22681 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC05207 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC11939 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC22518 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC20738 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC17272 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC19615 weak ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC22499 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00021-A-0 31293 ND DRSC18907 weak ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 31293 HFA18685 0.4 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 31293 HFA18685 -0.5 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
31293 FBgn0004647 N np sp none no library: DGC1
31293 FBgn0001994, FBgn0004647 crp, N RH54175 sp none no library: DGC2
31293 FBgn0004647 N LD34134 sp none no library: DGC1
GR00049-A-0 31293 FBgn0004647 DRSC18685 2 Decreased NFAT translocation no NFAT signaling A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT. Gwack et al. 2006 16511445 Cell line S2R+ NFAT translocation High content (microscopy) DRSC dsRNA Visual inspection np
31293 CG3936 N np np Defects in nervous system development no “Known” phenotype
31293 CG3936 N np np Heart patterning defects no
GR00065-A 31293 CG3936 N N np none no Dendrite pattern formation Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. Parrish et al. 2006 16547170 Tissue GAL4221 mCD8 protein expression Fluorescence Custom-made Transcription factors UAS-IR construct np Phenotypes in multiple blind tests Additional information about secondary screens
GR00128-A-2 31293 FBgn0004647 CG3936 DRSC37611 sp none no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00134-A-1 31293 CG3936 N 27229 np Flightless yes Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 31293 CG3936 N 1112 np Lethal yes late pupal Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 31293 CG3936 N 27228 np Lethal yes late pupal Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-2 31293 CG3936 N np np Wing posture / locomotion / ability to fly defect or lethal / semi-lethal yes Muscle morphogenesis and function (2) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection Custom-made Selected genes UAS-IR construct rp S19 > 0.5
GR00134-A-4 31293 CG3936 N 27229 np none no Muscle morphogenesis and function (4) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 F-actin protein expression Fluorescence np Selected genes UAS-IR construct np np
GR00135-A-1 31293 CG3936 N 1112 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 31293 CG3936 N 27229 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 31293 CG3936 1112 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00138-A-1 31293 CG3936 27229 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00139-A 31293 N 27229 sp Lethal yes Fly viability and adult morphology A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Dietzl et al. 2007 17625558 Organism Act5C-GAL4 Adult morphology and viability Visual inspection Custom-made Selected and random genes UAS-IR construct Phenotype strength np Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1 31293 CG3936 N 27228 1.6774193548387 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-1 31293 CG3936 N 2H6 T1 lethal Lethal temperature: 18 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 31293 CG3936 N 2H6 T1 no phenotype none temperature: 18 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 31293 CG3936 N 2H6 T1 lethal Lethal temperature: 25 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 31293 CG3936 N 2H6 T1 lethal Lethal, escapers mild gain of bristles temperature: 25 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 31293 CG3936 N 2H6 T1 lethal Lethal temperature: 29 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 31293 CG3936 N 2H6 T1 lethal Lethal, escapers no phenotype temperature: 29 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 31293 CG3936 N 2H6 T1 medium to strong Gain of bristles, m only temperature: 18 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 31293 CG3936 N 2H6 T1 lethal Lethal temperature: 29 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 31293 CG3936 N 2H6 T1 lethal Lethal temperature: 25 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 31293 CG3936 N 2H6 T1 lethal Lethal, escapers mild gain of bristles temperature: 29 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 31293 CG3936 N 2H6 T1 lethal Lethal, escapers no phenotype temperature: 18 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 31293 CG3936 N 2H6 T1 lethal Lethal, escapers with rare gain of bristles temperature: 25 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 31293 CG3936 N 2H6 T1 medium to strong Gain of bristles, m only, rare notum death malformation temperature: 18 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 31293 CG3936 N 2H6 T1 lethal Lethal, escapers loss of bristles and notum death malformation temperature: 18 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 31293 CG3936 N 2H6 T1 medium to strong Gain of bristles, some notum death malformation temperature: 25 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 31293 CG3936 N 2H6 T1 medium to strong Notum death malformation, loss of bristles temperature: 25 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 31293 CG3936 N 2H6 T1 medium to strong Notum death malformation, loss of bristles temperature: 29 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 31293 CG3936 N 2H6 T1 medium to strong Notum death malformation, loss of bristles, duplic ated hairs temperature: 29 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-4 31293 CG3936 27228 sp Loss of bristles, notum malformation death, lateral inhibition yes partially lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 31293 CG3936 1112 sp Gain of bristles, loss of bristles, lateral inhibition yes partially lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 31293 CG3936 27229 sp Gain of bristles, loss of bristles, notum malformation death, lateral inhibition yes not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-5 31293 CG3936 1112 np Wing margin notching, broad delta-like veins, wrinkled wings yes Notch pathway regulation (5) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue MS1096-GAL4 Wing morphology and viability Visual inspection np Selected genes UAS-IR construct Visual inspection np
GR00144-A-5 31293 CG3936 27228 np Wing margin notching, broad delta-like veins, wrinkled, small wings yes Notch pathway regulation (5) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue MS1096-GAL4 Wing morphology and viability Visual inspection np Selected genes UAS-IR construct Visual inspection np
GR00144-A-5 31293 CG3936 27229 np Wing margin notching, broad delta-like veins, wrinkled wings yes Notch pathway regulation (5) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue MS1096-GAL4 Wing morphology and viability Visual inspection np Selected genes UAS-IR construct Visual inspection np
GR00144-A-6 31293 CG3936 1112 np Decreased Su(H) protein expression yes Notch pathway regulation (6) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue ptc-GAL4 Su(H) protein expression X-gal np Selected genes UAS-IR construct Visual inspection np
GR00144-A-6 31293 CG3936 27229 np Decreased Su(H) protein expression yes Notch pathway regulation (6) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue ptc-GAL4 Su(H) protein expression X-gal np Selected genes UAS-IR construct Visual inspection np
GR00144-A-6 31293 CG3936 27228 np Decreased Su(H) protein expression yes Notch pathway regulation (6) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue ptc-GAL4 Su(H) protein expression X-gal np Selected genes UAS-IR construct Visual inspection np
GR00144-A-7 31293 CG3936 1112 np Too many sensory organ precursor cells no Notch pathway regulation (7) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 Number and size of sensory organ precursor cells Fluorescence np Selected genes UAS-IR construct Cell number and size np
GR00144-A-7 31293 CG3936 27228 np Too many sensory organ precursor cells no Notch pathway regulation (7) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 Number and size of sensory organ precursor cells Fluorescence np Selected genes UAS-IR construct Cell number and size np
GR00144-A-7 31293 CG3936 27229 np Too many sensory organ precursor cells no Notch pathway regulation (7) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 Number and size of sensory organ precursor cells Fluorescence np Selected genes UAS-IR construct Cell number and size np
GR00189-A-1 31293 FBgn0004647 N BKN22986 np Downregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 31293 FBgn0004647 N 1112 sp Downregulation of Notch pathway; adult wing veins: broader; adult wing margin: notches; adult wing posterior compartment: crumbled, blister; wing disc NRE:EGFP: down yes en-GAL4 Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 31293 FBgn0004647 N np np none yes Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00190-A-1 31293 FBgn0004647 N 27228 0.168 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00218-S 31293 CG3936 N AMB19813 -0.07 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00238-S-1 31293 CG3936 Notch 3936R-2 sp none no library: NIG Muscle development and maintenance The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. Perkins et al. 2014 25977760 Tissue Mef2-GAL4 Climbing ability and viability Negative geotaxis assay and visual inspection np Selected genes UAS-IR construct Complex, sp Complex criteria Author-submitted data. Published in Scientific Data 1, Article number: 140002.
GR00239-A-1 31293 CG3936 3936R np Lethal no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 31293 CG3936 N 1112 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 31293 CG3936 N 27228 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 31293 CG3936 N 100002 0.052392417070575 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 31293 FBgn0004647 N DRSC18685 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 31293 CG3936 N 100002 np Effect on follicular epithelium morphology further phenotype data in classification screen Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00315-A-2 31293 CG3936 N 100002 np Defect in formation and maturation phase Follicular epithelium development (2) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Selected genes UAS-IR construct Visual inspection np For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00335-A 31293 FBgn0004647 N HMS00001 np F1 lethal F1 embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 31293 FBgn0004647 N GL00092 np F1 lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 31293 FBgn0004647 N JF01693 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 31293 FBgn0004647 N JF02959 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 31293 FBgn0004647 N JF01891 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 31293 FBgn0004647 N HMS00015 np F1 lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 31293 FBgn0004647 N JF01637 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 31293 FBgn0004647 N HMS00009 np F1 lethal F1 embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
31293 FBgn0004647 np np sp none
GR00367-S 31293 CG3936 27229 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 31293 CG3936 100002 -0.48921705684558137 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A 31293 100002 0.48 none Regulation of olfactory memory formation Identification of genes that promote or inhibit olfactory memory formation in Drosophila. Walkinshaw et al. 2015 25644700 Organism Nsyb-GAL4 T-maze performance Visual inspection VDRC Selected genes UAS-IR construct Performance index, sp High performer in olfactory memory formation: > Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A 31293 CG3936 BL28981 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 GD27229 Lethal excess of ISC or EB Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 BL27988 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 BL33621 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 GD7077 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 BL7077 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 BL33611 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 BL7078 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 GD1112 none excess of ISC or EB Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 KK100002 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 GD27228 none excess of ISC or EB Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 31293 CG3936 BL33616 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.