Gene2Function

RNAi

Gene Info

Species:Fly (Drosophila melanogaster)
GeneID:31577
Symbol:CG3847
Description:uncharacterized protein

DataSource: http://genomernai.dkfz.de/v16/genedetails/31577

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	31577	FBgn0029867	CG3847	DRSC17444	-0.080179922	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31577	FBgn0029867	CG3847	DRSC17444	-0.089428956	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31577	FBgn0029867	CG3847	DRSC18327	1.641180659	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31577	FBgn0029867	CG3847	DRSC18327	0.907054244	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31577	FBgn0029867	CG3847	DRSC18327	1.101493798	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	31577	FBgn0029867	CG3847	DRSC18327	1.220479472	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00014-A	31577		CG3847	DRSC17444	strong	Decreased Flag-Mad nuclear accumulation		no		Dpp pathway regulation	Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads.	Xu et al.	2007	17785517	Cell line	S2R+	Flag-Mad protein expression and subcellular location	Fluorescence	rp	Genome-wide	dsRNA	Visual inspection	np
GR00021-A-0	31577	FBgn0029867		DRSC18327	weak	ERK activation reduced		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00031-A-1	31577			HFA18327	-0.2	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	31577			HFA18327	0.2	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
	31577	FBgn0029867	CG3847	GM08802	sp	none		yes	library: DGC1
	31577	FBgn0029867	CG3847	RH38929	sp	Decreased viability		yes	library: DGC2
GR00048-A-2	31577	FBgn0029867	CG3847	np	1.247	none		yes		Cell size and cell-cycle regulation (2)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	Bjӧrklund et al.	2006	16496002	Cell line	S2	Proliferation and viability	Colorimetrics		Selected genes	dsRNA	MTS metabolic activity	> 2.5 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3	31577	FBgn0029867	CG3847	np	464	none		no		Cell size and cell-cycle regulation (3)	Identification of pathways regulating cell size and cell-cycle progression by RNAi.	Bjӧrklund et al.	2006	16496002	Cell line	S2	Cell number	Fluorescence		Selected genes	dsRNA	Average cell number per field	> 2 standard deviations	Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00062-A	31577	FBgn0029867	CG3847	np	4	Decreased Ca2+ influx				Ca2+ channel regulation	CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.	Vig et al.	2006	16645049	Cell line	S2R+	Ca2+ influx	Fluorescence	np	Genome-wide	dsRNA	Complex, sp	>
GR00128-A-2	31577	FBgn0029867	CG3847	DRSC35303	sp	none		no		Combinatorial effect with methyl methanesulphonate (2)	A network of conserved damage survival pathways revealed by a genomic RNAi screen.	Ravi et al.	2009	19543366	Cell line	Kc167	Viability (synthetic lethal)	Luminescence	Version 1	Selected genes	dsRNA	rp	rp
GR00214-A-1	31577	FBgn0029867	CG3847	DRSC18327	-0.38	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	31577	FBgn0029867	CG3847	DRSC18327	1	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	31577	FBgn0029867	CG3847	DRSC18327	1.18	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	31577	FBgn0029867	CG3847	DRSC18327	0.17	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00218-S	31577	CG3847		AMB30961	0.72	none		no		Hippo pathway regulation	Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila	Wehr et al.	2013	23263283	Cell line	S2R+	Hippo pathway reporter	Luminescence	np	Genome-wide	dsRNA	Z-score	> 3 OR < -3	Author-submitted data. Primary screen
GR00239-A-1	31577	CG3847		3847R	np	Lethal		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	31577	CG3847	CG3847	101837	np	none			distorted ovaries	piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	31577	CG3847	CG3847	101837	-2.187094887366995	Strongly increased transposon expression				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	31577	np	np	DRSC18327	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1	31577	CG3847	CG3847	101837	np	Effect on follicular epithelium morphology			further phenotype data in classification screen	Follicular epithelium development (1)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Random genes	UAS-IR construct	Visual inspection	np	Further classification screen available, see follicular epithelium development (2).
GR00315-A-2	31577	CG3847	CG3847	101837	np	Defect in formation and maturation phase				Follicular epithelium development (2)	A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche	Berns et al.	2014	24762813	Tissue	traffic jam-GAL4	Follicular epithelium morphology	Fluorescence	VDRC KK	Selected genes	UAS-IR construct	Visual inspection	np	For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
	31577	np	np	HFA18327	0.2	none			fold change: 0.91
	31577	FBgn0029867	np	np	sp	none
GR00367-S	31577	CG3847		101837	np	liquid clearance defect			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00379-A-1	31577	CG3847		101837	4.570626475451219	High decrease in nos and yTub37c expression			No eggs/larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2	31577	CG3847		101837	np	No germline, 100%				Defects in female germline	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	Vasa AND 1B1 expression	Immunofluorescence	VDRC	Selected genes	UAS-IR construct	np	np	Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A	31577			101837	0.1241552989925074	Low performer in olfactory memory formation			Wing deformity	Regulation of olfactory memory formation	Identification of genes that promote or inhibit olfactory memory formation in Drosophila.	Walkinshaw et al.	2015	25644700	Organism	Nsyb-GAL4	T-maze performance	Visual inspection	VDRC	Selected genes	UAS-IR construct	Performance index, sp	High performer in olfactory memory formation: >	Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A	31577	CG3847		KK101837		Lethal				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.