| GR00002-A | 32777 | FBgn0027605 | CG6842 | DRSC19338 | 1.20054338 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 32777 | FBgn0027605 | CG6842 | DRSC19338 | 0.543926712 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00021-A-0 | 32777 | FBgn0027605 | | DRSC19338 | strong | ERK activation reduced | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 32777 | | | HFA19338 | 1.9 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 32777 | | | HFA19338 | 1.8 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00038-A-0 | 32777 | FBgn0027605 | Vps4 | np | *** | Fewer infected cells, less vacuolar escape, fewer bacteria per cell | | no | | L. monocytogenes infection | Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. | Cheng et al. | 2005 | 16157870 | Cell line | S2 | L. monocytogenes infection | High content (microscopy) | Custom-made | | dsRNA | Visual inspection | np | | GR00038-A-0 | 32777 | FBgn0027605 | Vps4 | np | **** | Escape pff LLO- strain | | no | | L. monocytogenes infection | Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. | Cheng et al. | 2005 | 16157870 | Cell line | S2 | L. monocytogenes infection | High content (microscopy) | Custom-made | | dsRNA | Visual inspection | np | | GR00040-A-0 | 32777 | FBgn0027605 | | DRSC19338 | 3.5 | Increased Wnt reporter activity | | no | | Wnt signaling | Functional genomic analysis of the Wnt-wingless signaling pathway. | DasGupta et al. | 2005 | 15817814 | Cell line | Clone 8 | Wnt signaling | Dual luciferase | DRSC | | dsRNA | Z-score | > | | GR00047-A-1 | 32777 | FBgn0027605 | CG6842 | DRSC19338 | sp | Decreased horseradish peroxidase protein expression | | no | | Constitutive protein secretion and Golgi organization (1) | Functional genomics reveals genes involved in protein secretion and Golgi organization. | Bard et al. | 2006 | 16452979 | Cell line | S2 | Horseradish peroxidase protein expression | Luminescence | | Genome-wide | dsRNA | Z-score | < -1.5 | | | 32777 | FBgn0027605, FBgn0004045 | CG6842, Yp1 | GH02678 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | GR00051-A-1 | 32777 | CG6842 | CG6842 | np | np | Decreased Candida albicans phagocytosis | | yes | validated (26 % cells phagocytosing) | Candida albicans phagocytosis (1) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | C. albicans phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing C. albicans | < | | GR00051-A-2 | 32777 | CG6842 | CG6842 | np | 30 | Decreased Escherichia coli phagocytosis | | yes | | Candida albicans phagocytosis (2) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | Escherichia coli phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing E. coli | < | | GR00051-A-3 | 32777 | CG6842 | CG6842 | np | 34 | Decreased latex beads phagocytosis | | yes | | Candida albicans phagocytosis (3) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | Latex beads phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing latex beads | < | | GR00069-A-0 | 32777 | FBgn0027605 | Vps4 | np | np | Increased M. smegmatis infection | | no | | M. smegmatis infection | ESCRT factors restrict mycobacterial growth. | Philips et al. | 2008 | 18287038 | Cell line | S2 | M. smegmatis infection | High content (microscopy) | DRSC | | dsRNA | np | np | | GR00130-A | 32777 | FBgn0027605 | CG6842 | np | <= -2 | Decreased mutant human huntingtin aggregation | | no | | Mutant human huntingtin aggregation | RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. | Doumanis et al. | 2009 | 19789644 | Cell line | BG2-c2 | Nhtt(62Q)EGFP aggregate number and size | Fluorescence | OpenBiosystems RNAi library | Selected genes | dsRNA | Z-score | Suppressor: < | | GR00131-A-1 | 32777 | | CG6842 | DRSC25068 | > 75 | Altered mitochondrial Ca2+ and/or H+ levels | | yes | | Mitochondrial Ca2+/H+ antiporter regulation (1) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Genome-wide | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00131-A-1 | 32777 | | CG6842 | DRSC19338 | > 75 | Altered mitochondrial Ca2+ and/or H+ levels | | yes | | Mitochondrial Ca2+/H+ antiporter regulation (1) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Genome-wide | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00131-A-2 | 32777 | | CG6842 | DRSC19338, DRSC31082 | > 75 | Altered mitochondrial Ca2+ and/or H+ levels | | no | | Mitochondrial Ca2+/H+ antiporter regulation (2) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Selected genes | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00134-A-1 | 32777 | CG6842 | CG6842 | 35125 | np | Lethal | | yes | early pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 32777 | CG6842 | CG6842 | 35126 | np | Lethal | | yes | late larval | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-2 | 32777 | CG6842 | CG6842 | np | np | Wing posture / locomotion / ability to fly defect or lethal / semi-lethal | | yes | | Muscle morphogenesis and function (2) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | Custom-made | Selected genes | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-3 | 32777 | CG6842 | CG6842 | 35125, 35126 | np | Fading Z-line | | no | | Muscle morphogenesis and function (3) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | ZCL0663 protein expression | Fluorescence | np | Selected genes | UAS-IR construct | np | np | | GR00135-A-1 | 32777 | CG6842 | CG6842 | 35126 | -1.17 | Developmentally lethal | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 32777 | CG6842 | CG6842 | 35125 | -1.17 | Developmentally lethal | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 32777 | CG6842 | | 35126 | 0.15 | Pupal lethal | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 32777 | CG6842 | CG6842 | 35125 | -1.59550561797752 | Decreased viability after Serratia marcescens infection | | yes | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00142-A-2 | 32777 | CG6842 | CG6842 | np | sp | none | | yes | | Serratia marcescens infection (2) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Tissue | HML-GAL4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Complex, sp | Significantly reduced survival (p < 0.05) | | GR00142-A-6 | 32777 | CG6842 | CG6842 | 35126 | np | Larval lethality or failure to eclose | | no | | Serratia marcescens infection (6) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Tissue | NP1-GAL4 | Viability | Sugar diet in the absence of bacteria | np | Selected genes | UAS-IR construct | np | np | | GR00144-A-4 | 32777 | CG6842 | | 35125 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 32777 | CG6842 | | 35126 | sp | Completely lethal (before pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00170-A-1 | 32777 | | Vps4 | DRSC19338 | -1.7 | Decreased HIF dependent transcription with desferrioxamine | | yes | | HIF-dependent transcription (1) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 1.0 library | Genome-wide | dsRNA | Z-score | < | | GR00189-A-1 | 32777 | FBgn0027605 | CG6842 | BKN21773 | np | Upregulation of Notch pathway | | yes | | Notch pathway regulation (1) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Cell line | S2 | Notch pathway reporter | Luminescence | BKN | Genome-wide | dsRNA | Complex, sp | Complex criteria | | GR00189-A-3 | 32777 | FBgn0027605 | Vps4 | np | np | Upregulation of Notch pathway | | yes | | Notch pathway regulation (3) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | GMR-GAL4 | Notch pathway reporter | Fluorescence | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Average fluorescent intensity | Complex criteria | | GR00189-A-4 | 32777 | FBgn0027605 | Vps4 | 35125 | very strong | Lethal | | no | en-GAL4 | Notch pathway regulation (4) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | en-GAL4 and C96-GAL4 | Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability | Fluorescence and visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00214-A-1 | 32777 | FBgn0027605 | Vps4 | DRSC19338 | -5.01 | Decreased ERK phosphorylation after EGF stimulation | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 32777 | FBgn0027605 | Vps4 | DRSC19338 | 0.12 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 32777 | FBgn0027605 | Vps4 | DRSC19338 | -2.68 | Decreased ERK phosphorylation after sSpitz and EGF stimulation | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 32777 | FBgn0027605 | Vps4 | DRSC19338 | -3.6 | Decreased ERK phosphorylation after sSpitz and EGF stimulation | 30 minutes sSpitz stimulation | yes | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00216-A | 32777 | FBgn0027605 | Vps4 | BKN21773 | 1.2 | none | | no | | Wnt proteins secretion | p24 proteins are required for secretion of Wnt ligands. | Buechling et al. | 2011 | 22094269 | Cell line | S2R+ | Wg pathway reporter | Luminescence | BKN | Cellular transporting machinery genes | dsRNA | Z-score | > 2.5 OR < -2.5 | Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4) | GR00218-S | 32777 | CG6842 | | AMB18809 | 1.32 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 32777 | CG6842 | | 6842R | np | Decreased Chp protein expression | | no | not detectable Chp level | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00282-A | 32777 | CG6842 | Vps4 | 35125 | np | none | | | rudimentary ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 32777 | CG6842 | Vps4 | 35126 | np | none | | | rudimentary ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 32777 | CG6842 | Vps4 | 105977 | -2.465189037348285 | Strongly increased transposon expression | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 32777 | np | np | DRSC19338 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00314-A | 32777 | FBgn0027605 | Vps4 | RNA002949 | 67.27 | Decreased MAPK phosphorylation | | | | RAS-related MAPK activation | A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila | Ashton-Beaucage et al. | 2014 | 24643257 | Cell line | S2 | MAPK phosphorylation | Fluorescence | Custom-made | Genome-wide | dsRNA | pMAPK signal (%GFP dsRNA) | np | S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai | GR00315-A-1 | 32777 | CG6842 | Vps4 | 105977 | np | Effect on follicular epithelium morphology | | | further phenotype data in classification screen | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 32777 | CG6842 | Vps4 | 105977 | np | Defect in formation and maturation phase | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | GR00335-A | 32777 | FBgn0027605 | Vps4 | HM04061 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 32777 | FBgn0027605 | np | np | -2.89 | Increased transposon expression | | | fold change: 13.22 | | | | | | | | | | | | | | | | | 32777 | FBgn0027605 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00339-A-2 | 32777 | FBgn0027605 | Vps4 | 35126 | na | Developmental defect | | | | piRNA pathway regulation (2) | A Genome-wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary piRNA Biogenesis in Drosophila | Muerdter et al. | 2013 | 23665228 | Tissue | traffic jam GAL4 | Gypsy and ZAM transposon expression | qPCR | VDRC (GD, KK) and TRiP | Selected genes | UAS-IR construct | Fold change | > | Following transposons were used for scoring: Gypsy for KK and ZAM for GD RNAi lines. TRIP reagents als TRIP_11111 dargestellt, Suchbarkeit? Developmental defect: wurden nicht gemessen, passt np als Score? | GR00367-S | 32777 | CG6842 | | 35126 | np | liquid clearance defect | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 32777 | CG6842 | | 105977 | 0.2166456282848687 | none | | | Intermediate eggs/larvae level | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00379-A-2 | 32777 | CG6842 | | 105977 | np | Egg chamber defects in 50%; No phenotype in 50% | | | | Defects in female germline | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | Vasa AND 1B1 expression | Immunofluorescence | VDRC | Selected genes | UAS-IR construct | np | np | Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 32777 | CG6842 | | GD35125 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |