| GR00002-A | 32794 | FBgn0004028 | wupA | DRSC20385 | -0.333072976 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 32794 | FBgn0004028 | wupA | DRSC20385 | -0.094112035 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 32794 | FBgn0004028 | wupA | DRSC20385 | -0.378487409 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 32794 | FBgn0004028 | wupA | DRSC20385 | 1.697922375 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 32794 | FBgn0004028 | wupA | DRSC22547 | -8,67E-05 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 32794 | FBgn0004028 | wupA | DRSC22547 | 1.079714801 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00005-A-0 | 32794 | FBgn0004028 | | DRSC20385 | weak | Centrosome clustering defects | | no | | Multipolar division | Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. | Kwon et al. | 2008 | 18662975 | Cell line | S2 | Centrosome clustering | High content (microscopy) | DRSC | | dsRNA | Visual inspection | np | | GR00007-A-0 | 32794 | FBgn0004028 | | DRSC20385 | np | Lamellar structures | | no | | Neural outgrowth | Identification of neural outgrowth genes using genome-wide RNAi. | Sepp et al. | 2008 | 18604272 | Cell line | Primary neural cells | Morphology | High content (microscopy) | DRSC | | dsRNA | Visual inspection | np | | GR00014-A | 32794 | | wupA | DRSC20385 | strong | Decreased Flag-Mad nuclear accumulation | | no | | Dpp pathway regulation | Msk is required for nuclear import of TGF-{beta}/BMP-activated Smads. | Xu et al. | 2007 | 17785517 | Cell line | S2R+ | Flag-Mad protein expression and subcellular location | Fluorescence | rp | Genome-wide | dsRNA | Visual inspection | np | | GR00031-A-1 | 32794 | | | HFA20385 | 0 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 32794 | | | HFA20385 | -0.3 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 32794 | FBgn0004028 | wupA | HL08042 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | GR00134-A-1 | 32794 | CG7178 | wupA | 34196 | np | Lethal | | yes | embryonic | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-3 | 32794 | CG7178 | wupA | 34196 | np | Rounded muscles, undefined larval sarcomere morphology | | no | | Muscle morphogenesis and function (3) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | ZCL0663 protein expression | Fluorescence | np | Selected genes | UAS-IR construct | np | np | | GR00135-A-1 | 32794 | CG7178 | wupA | 34196 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 32794 | CG7178 | | 34196 | 0.75 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 32794 | CG7178 | wupA | 34196 | 1.73684210526315 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 32794 | CG7178 | | 34196 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00159-A | 32794 | FBgn0004028 | wupA | DRSC20385, DRSC22547, DRSC29330, DRSC31986 | np | Muscles spread, actin and myosin filaments lack striation | | no | | Muscle assembly and maintenance | RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. | Bai et al. | 2008 | 18359903 | Primary cells | primary embryonic cells | Muscle cell morphology | Fluorescence | np | Selected and random genes | dsRNA | Percentage of muscles with a given phenotype | Severe: > 80 %; medium: ~ 50 % | | GR00190-A-1 | 32794 | FBgn0004028 | wupA | 34196 | -0.231 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00218-S | 32794 | CG7178 | wupA | AMB33401 | -1.2 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00238-S-1 | 32794 | CG7178 | troponin-I | 34196 | sp | Embryo lethal | | yes | library: VDRC | Muscle development and maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | Mef2-GAL4 | Climbing ability and viability | Negative geotaxis assay and visual inspection | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00238-S-2 | 32794 | CG7178 | troponin-I | 34196 | sp | Climbing defect | | no | library: VDRC | Muscle maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | TARGET Mef2-GAL4 | Climbing ability | Negative geotaxis assay | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00311-A | 32794 | FBgn0004028 | wupA | DRSC20385 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | | 32794 | FBgn0004028 | np | np | sp | none | | | | | | | | | | | | | | | | | | |