Gene2Function

RNAi

Gene Info

Species:Fly (Drosophila melanogaster)
GeneID:32855
Symbol:fu
Description:fused

DataSource: http://genomernai.dkfz.de/v16/genedetails/32855

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Stable_ID	Entrez_ID	Gene_ID	Gene_Symbol	Reagent_ID	Score	Phenotype	Conditions	Follow_Up	Comment	Screen_Title	Publication_Title	Authors	Publication_Year	Pubmed_ID	Biosource	Biomodel	Assay	Method	Library	Scope	Reagent_Type	Score_Type	Cutoff	Notes
GR00002-A	32855	FBgn0001079	fu	DRSC20333	-0.785801739	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A	32855	FBgn0001079	fu	DRSC20333	-0.174409715	none		no		Lipid storage	COPI complex is a regulator of lipid homeostasis.	Beller et al.	2008	19067489	Cell line	Kc167	Nuclear to lipid droplet cross-sectional area	Fluorescence	np	Genome-wide	dsRNA	B-score	> 2 OR < -1.7	Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0	32855	FBgn0001079		DRSC20333	moderate	ERK activation reduced		no		ERK signaling	A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.	Friedman et al.	2006	17086199	Cell line	S2R+	ERK signaling	Fluorescence	DRSC		dsRNA	Weak, moderate, strong	np
GR00030-A-1	32855	CG6551	fu	fu	np	none		yes		Cell cycle regulation (1)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content	Flow cytometry	rp	Kinases	dsRNA	Confidence intervals	Complex criteria
GR00030-A-2	32855	CG6551	fu	fu	np	none		no		Cell cycle regulation (2)	Genome-wide survey of protein kinases required for cell cycle progression.	Bettencourt-Dias et al.	2004	15616552	Cell line	S2	Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression	Flow cytometry	rp	Kinases and kinase regulators	dsRNA	np	np
GR00031-A-1	32855			HFA20333	1.1	none		yes		Cell growth and viability (1)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	Kc167	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00031-A-2	32855			HFA20333	1.3	none		no		Cell growth and viability (2)	Genome-wide RNAi analysis of growth and viability in Drosophila cells.	Boutros et al.	2004	14764878	Cell line	S2R+	Cell number and viability	Luminescence	Custom-made (HFA)	Genome-wide	dsRNA	Z-score	>
GR00045-A-0	32855	FBgn0001079		DRSC20333	-3.03	Decreased Hedgehog reporter activity		no		Hedgehog signaling	A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway.	Nybakken et al.	2005	16311596	Cell line	Clone 8	Hedgehog signaling	Dual luciferase	DRSC		dsRNA	Z-score	>
	32855	FBgn0001079	fu	RE28848	sp	none		no	library: DGC2
	32855	FBgn0001079	fu	CT20401	sp	none		no	library: PHOSPHO
GR00062-A	32855	FBgn0001079	fu	np	2	Decreased Ca2+ influx				Ca2+ channel regulation	CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.	Vig et al.	2006	16645049	Cell line	S2R+	Ca2+ influx	Fluorescence	np	Genome-wide	dsRNA	Complex, sp	>
GR00134-A-1	32855	CG6551	fu	27662	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00134-A-1	32855	CG6551	fu	27663	np	none		no		Muscle morphogenesis and function (1)	Systematic genetic analysis of muscle morphogenesis and function in Drosophila.	Schnorrer et al.	2010	20220848	Tissue	Mef2-GAL4	Posture, locomotion, flight and viability	Visual inspection	np	Genome-wide	UAS-IR construct	rp	S19 > 0.5
GR00135-A-1	32855	CG6551	fu	27663	-1.17	none		yes		Heat nociception (1)	A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene.	Neely et al.	2010	21074052	Organism	elav-GAL4	Noxious heat avoidance and viability	Fly count	np	Genome-wide	UAS-IR construct	Z-score	> 1.65	Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1	32855	CG6551		27662	1	none		no		Heart development and function (1)	A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.	Neely et al.	2010	20371351	Tissue	TinCΔ4 12a-Gal4	Viability	Fly count	np	Selected genes	UAS-IR construct	Developmental lethality	<
GR00139-A	32855		fu	27663	sp	Semi-lethal		no		Fly viability and adult morphology	A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.	Dietzl et al.	2007	17625558	Organism	Act5C-GAL4	Adult morphology and viability	Visual inspection	Custom-made	Selected and random genes	UAS-IR construct	Phenotype strength	np	Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1	32855	CG6551	fu	27663	-1.63013698630136	Decreased viability after Serratia marcescens infection		no		Serratia marcescens infection (1)	Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection.	Cronin et al.	2009	19520911	Organism	HSP70-GAL4; TubGAL80ts	Heat shock and viability	Fly count	np	Random genes	UAS-IR construct	Days life time (LT50)	< -1.5 SD OR > 2 SD
GR00144-A-4	32855	CG6551		27662	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00144-A-4	32855	CG6551		27663	0	none		no	not lethal	Notch pathway regulation (4)	Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.	Mummery-Widmer et al.	2009	19363474	Tissue	pnr-GAL4	External sensory organ morphology and viability	Visual inspection	np	Genome-wide	UAS-IR construct	Phenotype strength	np
GR00175-A	32855	CG6551	fu	DRSC32468	-0.518948696160855	Downregulation of Hh pathway		no		Hh pathway regulation	A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.	DasGupta et al.	2007	17903264	Cell line	rp	rp	Luminescence	DRSC-v	Selected genes	dsRNA	Average fractional change	Complex criteria
GR00175-A	32855	CG6551	fu	DRSC32467	-0.619799256611346	Downregulation of Hh pathway		no		Hh pathway regulation	A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila.	DasGupta et al.	2007	17903264	Cell line	rp	rp	Luminescence	DRSC-v	Selected genes	dsRNA	Average fractional change	Complex criteria
GR00190-A-1	32855	FBgn0001079	fu	27663	-0.01	none		no		Adiposity regulation (1)	Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate.	Pospisilik et al.	2010	20074523	Organism	Hsp70-GAL4;Tub-GAL80ts	Total fly triglyceride expression	Colorimetrics	np	Genome-wide	UAS-IR construct	Triglyceride change	Z-score > 1.65 after 3 screening rounds	Additional information about the primary screen
GR00191-A-1	32855	CG6551	fu	np	np	none		yes		Blood cell homeostasis regulation (1)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	srp-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00191-A-2	32855	CG6551	fu	np	np	none		no		Blood cell homeostasis regulation (2)	An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis.	Avet-Rochex et al.	2010	20540764	Tissue	hml∆-GAL4	Melanotic mass formation	Microscopy	np	Selected genes	UAS-IR construct	np	np
GR00201-A-1	32855	FBgn0001079	fu	CG6551 (UCSF), fu (Baum_Lab)	np	none		yes		Actin organization and cell morphology (1)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-2	32855	FBgn0001079	fu	CG6551 (UCSF), fu (Baum_Lab)	np	none		yes		Actin organization and cell morphology (2)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	S2R+	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-3	32855	FBgn0001079	fu	CG6551 (UCSF), fu (Baum_Lab)	np	none		yes		Actin organization and cell morphology (3)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	Kc167	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-4	32855	FBgn0001079	fu	CG6551 (UCSF), fu (Baum_Lab)	np	none		yes		Actin organization and cell morphology (4)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG2-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-5	32855	FBgn0001079	fu	CG6551 (UCSF), fu (Baum_Lab)	np	none		yes		Actin organization and cell morphology (5)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c1	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00201-A-6	32855	FBgn0001079	fu	CG6551 (UCSF), fu (Baum_Lab)	np	none		no		Actin organization and cell morphology (6)	Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology.	Liu et al.	2009	19265526	Cell line	BG3-c2	alpha-Tubulin and F-actin protein expression	Fluorescence	Custom-made	Kinases	dsRNA	Visual inspection	np
GR00202-A	32855	FBgn0001079	fu	np	sp	none		no		Vaccinia virus infection	A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.	Moser et al.	2010	20585561	Cell line	DL1	Virally encoded beta-galactosidase protein expression	Fluorescence	Custom-made	Kinases, phosphatases and selected genes	dsRNA	Z-score	< -2 in both duplicates	Author-reviewed data
GR00214-A-1	32855	FBgn0001079	fu	DRSC20333	0.44	none	0 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-1	32855	FBgn0001079	fu	DRSC20333	0.66	none	10 minutes EGF stimulation	yes		RTK-Ras-ERK pathway regulation (1)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	S2R+	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	32855	FBgn0001079	fu	DRSC20333	0.1	none	30 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00214-A-2	32855	FBgn0001079	fu	DRSC20333	-0.48	none	10 minutes sSpitz stimulation	no		RTK-Ras-ERK pathway regulation (2)	Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling.	Friedman et al.	2011	22028469	Cell line	Kc167	ERK phosphorylation	Fluorescence	np	Genome-wide	dsRNA	Z-score	> 1.5 OR < -1.5
GR00239-A-1	32855	CG6551		6551R	sp	none		no		Glycosylation regulation (1)	Identification of genes required for neural-specific glycosylation using functional genomics.	Yamamoto-Hino et al.	2010	21203496	Tissue	GMR-GAL4	Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability	Luminescence	np	Selected genes	UAS-IR construct	Z-score	> 3
GR00282-A	32855	CG6551	fu	27662	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A	32855	CG6551	fu	27663	np	none				piRNA pathway regulation	The Genetic Makeup of the Drosophila piRNA Pathway	Handler	2013	23665231	Tissue	tj-GAL4	Gypsy transposon expression	Microscopy	VDRC	Selected genes	UAS-IR construct	Visual inspection	np	See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A	32855	CG6551	fu	27662	0.42978792705276525	none				piRNA pathway regulation	A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway	Czech et al.	2013	23665227	Tissue	nos-GAL4	Transposon expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Weak < -1.5; strong: < -2	KK and GD libraries used (see phenotype data)
GR00311-A	32855	FBgn0001079	fu	DRSC20333	np	none				Actin and microtubule morphology	Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype	Rohn et al.	2011	21893601	Cell line	S2R+	Alpha-tubulin and F-actin protein expression	Fluorescence	DRSC	Genome-wide	dsRNA	Visual inspection	np	Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00335-A	32855	FBgn0001079	fu	GL00148	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	32855	FBgn0001079	fu	JF01579	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A	32855	FBgn0001079	fu	JF01490	np	none				Germline stem cell (GSC) regulation	A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal	Yan et al.	2014	24576427	Tissue	MTD-GAL4 or UAS-dcr2; nanos-GAL4	Spectrin and vasa protein expression	Fluorescence	TRiP	Selected genes	UAS-IR construct	Visual inspection	np	More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
	32855	FBgn0001079	np	np	sp	none
GR00367-S	32855	CG6551		27662	np	lethal or adult morphology phenotype			VDRC	Drosophila airway maturation	Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes	Hosono et al.	2015	26551273	Tissue	btl-GAL4	Liquid clearance defect and viability	Visual inspection	VDRC, Trip, NIG	Genome-wide	UAS-IR construct	Frequency	>30%
GR00370-A	32855	FBgn0001079	fu		0.86	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	32855	FBgn0001079	fu		-0.89	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00370-A	32855	FBgn0001079	fu		-0.21	none				Regulation of Nrf2 homolog CncC	Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes	Li et al.	2016	26911346	Cell line	S2	CncC reporter	Luminescence	HD2	Kinases	dsRNA	Z-Score	<-1.65 OR >1.65
GR00379-A-1	32855	CG6551		27662	-0.25673029663593666	none			No phenotype in egg laying/hatching or larvae in egg laying or larvae	Stem cell maintenance	Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation	Sanchez et al.	2016	26669894	Organism	nanos-GAL4	nos and yTub37c mRNA expression	qPCR	VDRC	Selected genes	UAS-IR construct	Z-score	Low: 1 - 2.5; Medium: 2.5 - 4; High: >	Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A	32855	CG6551		GD27663		none				Regulation of intestinal stem cells (ISC)	Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila	Zeng et al.	2015	25704823	Tissue	act5C-GAL4	Viability	Visual inspection	VDRC and BDSC	Genome-wide	UAS-IR construct	np	np	Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.