RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:35027
  • Symbol:CG12288
  • Description:uncharacterized protein
DataSource: http://genomernai.dkfz.de/v16/genedetails/35027

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 35027 FBgn0032620 CG12288 DRSC02155 -0.202951566 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 35027 FBgn0032620 CG12288 DRSC02155 0.88178395 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1 35027 HFA02155 0.2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 35027 HFA02155 0.4 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00035-A 35027 CG12288 CG12288 JP074 sp none no Alternative splicing Identification of alternative splicing regulators by RNA interference in Drosophila. Park et al. 2004 15492211 Cell line S2 dAdar, para and Dscam mRNA expression Reverse transcription PCR and autoradiography Custom-made RNA-binding protein genes and spliceosomal genes dsRNA np Alternative exon splicing for >
35027 FBgn0032620 CG12288 GM13065 sp none no library: DGC2
35027 FBgn0032620 CG12288 np 0.733 Decreased mitochondrial citrate synthase activity no
GR00137-A-1 35027 CG12288 np -0.6 none no decreased cell number or influenced reporter expression Regulated secretory pathway biogenesis (1) RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway. Asensio et al. 2010 21149569 Cell line S2 dVMAT protein cell surface expression Flow cytometry DmRNAi library version 1 Selected genes dsRNA Z-score >
GR00137-A-1 35027 CG12288 np 0.9 none no decreased cell number or influenced reporter expression Regulated secretory pathway biogenesis (1) RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway. Asensio et al. 2010 21149569 Cell line S2 dVMAT protein cell surface expression Flow cytometry DmRNAi library version 1 Selected genes dsRNA Z-score >
GR00218-S 35027 CG12288 AMB24528 -1.3 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00311-A 35027 FBgn0032620 CG12288 DRSC02155 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
35027 FBgn0032620 np np 0.13 none fold change: 0.94
35027 np np HFA02155 3.08 none fold change: 0.14