| GR00002-A | 35098 | FBgn0004413 | RpS26 | DRSC03420 | 2.415313863 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 35098 | FBgn0004413 | RpS26 | DRSC03420 | 1.266910663 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00004-A-0 | 35098 | | RpS26 | DRSC03420 | 0.52 | Endo-siRNA reporter downregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00004-A-0 | 35098 | | RpS26 | DRSC03420 | 1.04 | siRNA reporter downregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00010-A | 35098 | | RpS26 | np | np | Increased poly-(A+) mRNA nuclear accumulation | | no | <50% phenotype penetrance | mRNA nuclear export | Definition of global and transcript-specific mRNA export pathways in metazoans. | Farny et al. | 2008 | 18086857 | Cell line | S2R+ | Poly-(A+) mRNA nuclear accumulation | Fluorescence | Full genome dsRNA library | Genome-wide | dsRNA | Visual inspection | np | | GR00021-A-0 | 35098 | FBgn0004413 | | DRSC03420 | strong | ERK activation reduced | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 35098 | | RpS26 | HFA03420 | 3.9 | Decreased cell number and viability | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 35098 | | RpS26 | HFA03420 | 2.9 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 35098 | FBgn0004413 | RpS26 | DRSC03420 | medium | Binucleate cells with decreased viability | | no | | | | | | | | | | | | | | | | | GR00036-A | 35098 | CG10305 | RpS26 | np | weak | Increased GFP protein expression | | no | increased intracellular infection | Intracellular Listeria monocytogenes infection | Genome-wide RNAi screen for host factors required for intracellular bacterial infection. | Agaisse et al. | 2005 | 16020693 | Cell line | SL2 | GFP protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | | GR00039-A-0 | 35098 | FBgn0004413 | | DRSC03420 | np | Drosophila C virus infection reduced | | no | | Drosophila C virus infection | Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition. | Cherry et al. | 2005 | 15713840 | Cell line | S2 | Drosophila C virus infection | High content (microscopy) | DRSC | | dsRNA | Visual inspection | > 40% reduced infection | | GR00045-A-0 | 35098 | FBgn0004413 | | DRSC03420 | -2.61 | Decreased Hedgehog reporter activity | | no | | Hedgehog signaling | A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. | Nybakken et al. | 2005 | 16311596 | Cell line | Clone 8 | Hedgehog signaling | Dual luciferase | DRSC | | dsRNA | Z-score | > | | GR00047-A-1 | 35098 | FBgn0004413 | RpS26 | DRSC03420 | sp | Decreased horseradish peroxidase protein expression | | no | | Constitutive protein secretion and Golgi organization (1) | Functional genomics reveals genes involved in protein secretion and Golgi organization. | Bard et al. | 2006 | 16452979 | Cell line | S2 | Horseradish peroxidase protein expression | Luminescence | | Genome-wide | dsRNA | Z-score | < -1.5 | | | 35098 | FBgn0004413 | RpS26 | RE01079 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | GR00051-A-1 | 35098 | CG10305 | RpS26 | np | np | none | | no | | Candida albicans phagocytosis (1) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | C. albicans phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing C. albicans | < | | GR00062-A | 35098 | FBgn0004413 | RpS26 | np | 1 | Decreased Ca2+ influx | | | | Ca2+ channel regulation | CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. | Vig et al. | 2006 | 16645049 | Cell line | S2R+ | Ca2+ influx | Fluorescence | np | Genome-wide | dsRNA | Complex, sp | > | | GR00128-A-2 | 35098 | FBgn0004413 | CG10305 | DRSC30693 | sp | none | | no | | Combinatorial effect with methyl methanesulphonate (2) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Selected genes | dsRNA | rp | rp | | GR00131-A-1 | 35098 | | RpS26 | DRSC03420 | 25 - 50 | Altered mitochondrial Ca2+ and/or H+ levels | | no | | Mitochondrial Ca2+/H+ antiporter regulation (1) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Genome-wide | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00135-A-1 | 35098 | CG10305 | RpS26 | 16012 | 0.55 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 35098 | CG10305 | | 16012 | 0.08 | Larval lethal | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 35098 | CG10305 | RpS26 | 16012 | -2.66666666666666 | Decreased viability after Serratia marcescens infection | | yes | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00142-A-2 | 35098 | CG10305 | RpS26 | np | sp | none | | yes | | Serratia marcescens infection (2) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Tissue | HML-GAL4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Complex, sp | Significantly reduced survival (p < 0.05) | | GR00142-A-6 | 35098 | CG10305 | RpS26 | 16012 | np | Larval lethality or failure to eclose | | no | | Serratia marcescens infection (6) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Tissue | NP1-GAL4 | Viability | Sugar diet in the absence of bacteria | np | Selected genes | UAS-IR construct | np | np | | GR00144-A-4 | 35098 | CG10305 | | 16012 | sp | Completely lethal (before pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00170-A-1 | 35098 | | RpS26 | DRSC03420 | -8.3 | none | | no | decreased cell viability or Sanger collection sequence | HIF-dependent transcription (1) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 1.0 library | Genome-wide | dsRNA | Z-score | < | | GR00175-A | 35098 | CG10305 | RpS26 | DRSC30693 | -0.310573978636416 | Downregulation of Hh pathway | | no | | Hh pathway regulation | A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. | DasGupta et al. | 2007 | 17903264 | Cell line | rp | rp | Luminescence | DRSC-v | Selected genes | dsRNA | Average fractional change | Complex criteria | | GR00175-A | 35098 | CG10305 | RpS26 | DRSC30694 | -0.272475477964777 | Downregulation of Hh pathway | | no | | Hh pathway regulation | A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. | DasGupta et al. | 2007 | 17903264 | Cell line | rp | rp | Luminescence | DRSC-v | Selected genes | dsRNA | Average fractional change | Complex criteria | | GR00194-A | 35098 | CG10305 | RpS26 | np | np | none | | no | FFT: bottom 40, No. of corners: bottom 40 | S2 cell spreading | A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. | D'Ambrosio and Vale | 2010 | 21041442 | Cell line | S2U | alpha-tubulin and actin protein expression | Fluorescence | V2 RNAi library | Genome-wide | dsRNA | Complex, sp | Complex criteria | | GR00214-A-1 | 35098 | FBgn0261597 | RpS26 | DRSC03420 | 0.63 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 35098 | FBgn0261597 | RpS26 | DRSC03420 | -3.62 | Decreased ERK phosphorylation after EGF stimulation | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 35098 | FBgn0261597 | RpS26 | DRSC03420 | -0.18 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 35098 | FBgn0261597 | RpS26 | DRSC03420 | 2.75 | Increased ERK phosphorylation after sSpitz and EGF stimulation | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 35098 | CG10305 | RpS26 | AMB16289 | -0.45 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00273-A-1 | 35098 | FBgn0261597 | RpS26 | DRSC03420 | 3 | Effect on Polycomb (Pc) foci | | | | Polycomb group (PcG) proteins regulation (1) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Pc-GFP protein localization | Fluorescence | DRSC | Genome-wide and transcription factors | dsRNA | Visual inspection | 1 | Additional phenotypic clustering screen available. | GR00282-A | 35098 | CG10305 | RpS26 | 16012 | np | none | | | rudimentary ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 35098 | CG10305 | RpS26 | 109004 | np | none | | | rudimentary ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 35098 | CG10305 | RpS26 | 16012 | -1.81861273055438 | Weakly increased transposon expression | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00291-A | 35098 | | RpS26 | DRSC03420 | sp | none | | | eliminated based on control score | Hedgehog (hh) secretion regulation | A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila | Aikin et al. | 2012 | 22432040 | Cell line | S2 | Hedgehog protein expression | Luminescence | DRSC | Genome-wide | dsRNA | Z-score | < -2 OR > 2 by at least 1/3 Z-scores | 296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available. | GR00308-A | 35098 | CG10305 | RpS26 | np | 2 | Decreased cell aggregation | | | | Cadherin-mediated cell-cell adhesion | A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion | Toret et al. | 2014 | 24446484 | Cell line | S2 | Cell aggregation | Fluorescence | V2 RNAi library (Thermo Fisher Scientific) | Genome-wide | dsRNA | Visual inspection; average of 3 replicates; 0 | > 1.5 | Additional secondary screen available. S2 cells stably express DE-cadherin. | GR00311-A | 35098 | FBgn0004413 | RpS26 | DRSC03420 | np | Actin defect, other cellular phenotype | | | cell shape variable, decreased level of actin, decreased cell number, loss of cell monolayer | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00335-A | 35098 | FBgn0261597 | RpS26 | HMS00270 | np | F1 lethal | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 35098 | FBgn0261597 | np | np | 0.18 | none | | | fold change: 0.89 | | | | | | | | | | | | | | | | GR00379-A-1 | 35098 | CG10305 | | 16012 | 2.506732740305262 | Medium decrease in nos and yTub37c expression | | | No eggs/larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00379-A-2 | 35098 | CG10305 | | 16012 | np | No germline, 100% | | | | Defects in female germline | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | Vasa AND 1B1 expression | Immunofluorescence | VDRC | Selected genes | UAS-IR construct | np | np | Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 35098 | CG10305 | | GD16012 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 35098 | CG10305 | | BL33393 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |