| GR00001-A | 35762 | FBgn0033247 | Nup44A | DRSC07248 | -2.47 | Decreased cell size | | no | | Cell size regulation | PDGF/VEGF signaling controls cell size in Drosophila. | Sims et al. | 2009 | 19216764 | Cell line | S2R+ | F-actin and microtubule protein expression and DNA content | Fluorescence | rp | Genome-wide | dsRNA | Cell area Z-score | < -2 | | GR00002-A | 35762 | FBgn0033247 | Nup44A | DRSC07248 | 0.266802033 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 35762 | FBgn0033247 | Nup44A | DRSC07248 | 1.170659707 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00021-A-0 | 35762 | FBgn0033247 | | DRSC07248 | weak | ERK activation increased | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 35762 | | | HFA07248 | 0.4 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 35762 | | | HFA07248 | 0.3 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 35762 | FBgn0033247 | Nup44A | SD07614 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | GR00134-A-1 | 35762 | CG8722 | Nup44A | 40717 | np | Lethal | | no | early pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 35762 | CG8722 | Nup44A | 40717 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 35762 | CG8722 | | 40717 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 35762 | CG8722 | Nup44A | 40717 | -2.2375 | Decreased viability after Serratia marcescens infection | | yes | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00142-A-2 | 35762 | CG8722 | Nup44A | np | sp | none | | yes | | Serratia marcescens infection (2) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Tissue | HML-GAL4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Complex, sp | Significantly reduced survival (p < 0.05) | | GR00142-A-4 | 35762 | CG8722 | Nup44A | np | sp | Decreased viability after Serratia marcescens infection | | no | | Serratia marcescens infection (4) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Tissue | NP1-GAL4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Complex, sp | Significantly reduced survival (p < 0.05) | | GR00144-A-4 | 35762 | CG8722 | | 40717 | sp | Bristle morphology defects, notum malformation death | | no | partially lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00189-A-1 | 35762 | FBgn0033247 | Nup44A | BKN28228 | np | Upregulation of Notch pathway | | yes | | Notch pathway regulation (1) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Cell line | S2 | Notch pathway reporter | Luminescence | BKN | Genome-wide | dsRNA | Complex, sp | Complex criteria | | GR00189-A-2 | 35762 | FBgn0033247 | Nup44A | 40717 | sp | Upregulation of Notch pathway; wing disc size: overgrown; wing disc NRE:EGFP: up; wing disc myrRFP: accumulation, reduced; lethal | | yes | en-GAL4 | Notch pathway regulation (2) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | en-GAL4, C96-GAL4 and vg-GAL4 | Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability | Fluorescence and visual inspection | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00189-A-3 | 35762 | FBgn0033247 | Nup44A | np | np | none | | no | | Notch pathway regulation (3) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | GMR-GAL4 | Notch pathway reporter | Fluorescence | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Average fluorescent intensity | Complex criteria | | GR00191-A-1 | 35762 | CG8722 | Nup44A | np | np | Increased melanotic mass formation | | yes | | Blood cell homeostasis regulation (1) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-2 | 35762 | CG8722 | Nup44A | np | np | none | | yes | | Blood cell homeostasis regulation (2) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-3 | 35762 | CG8722 | Nup44A | np | 0 | none | | yes | | Blood cell homeostasis regulation (3) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00191-A-4 | 35762 | CG8722 | Nup44A | np | 0 | none | | yes | | Blood cell homeostasis regulation (4) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00191-A-5 | 35762 | CG8722 | Nup44A | np | 0 | none | | no | | Blood cell homeostasis regulation (5) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | cg-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00194-A | 35762 | CG8722 | Nup44A | np | np | none | | yes | FFT: bottom 40, No. of corners: bottom 40 | S2 cell spreading | A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis. | D'Ambrosio and Vale | 2010 | 21041442 | Cell line | S2U | alpha-tubulin and actin protein expression | Fluorescence | V2 RNAi library | Genome-wide | dsRNA | Complex, sp | Complex criteria | | GR00214-A-1 | 35762 | FBgn0033247 | Nup44A | DRSC07248 | 0.37 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 35762 | FBgn0033247 | Nup44A | DRSC07248 | 0.15 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 35762 | FBgn0033247 | Nup44A | DRSC07248 | -0.35 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 35762 | FBgn0033247 | Nup44A | DRSC07248 | -0.02 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00216-A | 35762 | FBgn0033247 | Nup44A | BKN28228 | 1.1 | none | | no | | Wnt proteins secretion | p24 proteins are required for secretion of Wnt ligands. | Buechling et al. | 2011 | 22094269 | Cell line | S2R+ | Wg pathway reporter | Luminescence | BKN | Cellular transporting machinery genes | dsRNA | Z-score | > 2.5 OR < -2.5 | Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4) | GR00217-A-1 | 35762 | CG8722 | Nup44a | np | medium | Decreased WgRluc and not changed sFluc protein expression | | no | | Wg pathway regulation (1) | A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. | Port et al. | 2011 | 21886182 | Cell line | S2R+ | WgRluc and sFluc protein expression | Luminescence | np | Genome-wide | dsRNA | Phenotype strength | Complex criteria | Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4) | GR00239-A-1 | 35762 | CG8722 | | 8722R | sp | none | | no | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00273-A-1 | 35762 | FBgn0033247 | Nup44A | DRSC07248 | 2 | Effect on Polycomb (Pc) foci | | | | Polycomb group (PcG) proteins regulation (1) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Pc-GFP protein localization | Fluorescence | DRSC | Genome-wide and transcription factors | dsRNA | Visual inspection | 1 | Additional phenotypic clustering screen available. | GR00282-A | 35762 | CG8722 | Nup44A | 106489 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 35762 | CG8722 | Nup44A | 40717 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 35762 | CG8722 | Nup44A | 106489 | -1.2524915137482915 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 35762 | FBgn0033247 | Nup44A | DRSC07248 | np | Other cellular phenotype | | | decreased cell size | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 35762 | CG8722 | Nup44A | 106489 | np | Effect on follicular epithelium morphology | | | further phenotype data in classification screen | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 35762 | CG8722 | Nup44A | 106489 | np | Defect in maturation phase | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | GR00335-A | 35762 | FBgn0033247 | Nup44A | HMS00736 | np | Oogenesis defect | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 35762 | FBgn0033247 | Nup44A | HMS01825 | np | Oogenesis defect | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 35762 | FBgn0033247 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 35762 | CG8722 | | 40717 | np | liquid clearance defect | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 35762 | CG8722 | | 106489 | 1.2897933899327252 | Low decrease in nos and yTub expression | | | No eggs/larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00379-A-2 | 35762 | CG8722 | | 106489 | np | Egg chamber defects in 80%; No phenotype in 20% | | | Validated by independent RNAi line | Defects in female germline | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | Vasa AND 1B1 expression | Immunofluorescence | VDRC | Selected genes | UAS-IR construct | np | np | Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 35762 | CG8722 | | BL32942 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 35762 | CG8722 | | GD40717 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |