| GR00002-A | 35924 | FBgn0033378 | tsu | DRSC07265 | 1.073997436 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 35924 | FBgn0033378 | tsu | DRSC07265 | 0.088172924 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00031-A-1 | 35924 | | | HFA07265 | -0.9 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 35924 | | | HFA07265 | 0.1 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00035-A | 35924 | CG8781 | tsu | JP038 | sp | none | | no | | Alternative splicing | Identification of alternative splicing regulators by RNA interference in Drosophila. | Park et al. | 2004 | 15492211 | Cell line | S2 | dAdar, para and Dscam mRNA expression | Reverse transcription PCR and autoradiography | Custom-made | RNA-binding protein genes and spliceosomal genes | dsRNA | np | Alternative exon splicing for > | | | 35924 | FBgn0033378 | tsu | RE09075 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | GR00051-A-1 | 35924 | CG8781 | tsu | np | np | none | | no | | Candida albicans phagocytosis (1) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | C. albicans phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing C. albicans | < | | GR00128-A-1 | 35924 | FBgn0033378 | CG8781 | DRSC07265 | sp | none | | no | hit according to not so stringent primary analysis | Combinatorial effect with methyl methanesulphonate (1) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Genome-wide | dsRNA | rp | rp | Additional information about the primary screen | GR00134-A-1 | 35924 | CG8781 | tsu | 36024 | np | Lethal | | no | early pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 35924 | CG8781 | tsu | 36025 | np | Lethal | | no | early pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 35924 | CG8781 | tsu | 36025 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 35924 | CG8781 | | 36025 | 0.9 | none | | yes | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00138-A-2 | 35924 | CG8781 | | 36025 | 10.21 | Decreased viability | | no | | Heart development and function (2) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00139-A | 35924 | | tsu | 36024 | sp | Lethal | | no | | Fly viability and adult morphology | A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. | Dietzl et al. | 2007 | 17625558 | Organism | Act5C-GAL4 | Adult morphology and viability | Visual inspection | Custom-made | Selected and random genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) | GR00139-A | 35924 | | tsu | 36025 | sp | Lethal | | no | | Fly viability and adult morphology | A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. | Dietzl et al. | 2007 | 17625558 | Organism | Act5C-GAL4 | Adult morphology and viability | Visual inspection | Custom-made | Selected and random genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) | GR00142-A-1 | 35924 | CG8781 | tsu | 36025 | -0.907692307692307 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 35924 | CG8781 | | 36024 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 35924 | CG8781 | | 36025 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00183-A | 35924 | FBgn0033378 | tsu | 36025 | sp | GFP aggregates number, GFP aggregates size, lethal | | no | | Self-renewal and differentiation in neural stem cells | Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. | Neumueller et al. | 2011 | 21549331 | Tissue | insc-GAL4 | Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability | Fluorescence | np | Genome-wide | UAS-IR construct | Phenotype strength | > 1 | Additional information about a secondary screen (KK library) | GR00190-A-1 | 35924 | FBgn0033378 | tsu | 36025 | 0.191 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00218-S | 35924 | CG8070, CG8781 | | AMB27071 | -0.57 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00218-S | 35924 | CG8781 | | AMB30765 | -0.67 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 35924 | CG8781 | | 8781R | sp | Increased mannose Chp glycosylation | | yes | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00239-A-2 | 35924 | CG8781 | | 8781R | np | none | | no | | Glycosylation regulation (2) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation | Luminescence | np | Selected genes | UAS-IR construct | p-value | < 0.005 | | GR00282-A | 35924 | CG8781 | tsu | 36024 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 35924 | CG8781 | tsu | 107385 | np | Increased transposon expression | | | distorted ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 35924 | CG8781 | tsu | 107385 | -1.6741556870572742 | Weakly increased transposon expression | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00308-A | 35924 | CG8781 | | np | 2.33 | Decreased cell aggregation | | | | Cadherin-mediated cell-cell adhesion | A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion | Toret et al. | 2014 | 24446484 | Cell line | S2 | Cell aggregation | Fluorescence | V2 RNAi library (Thermo Fisher Scientific) | Genome-wide | dsRNA | Visual inspection; average of 3 replicates; 0 | > 1.5 | Additional secondary screen available. S2 cells stably express DE-cadherin. | GR00311-A | 35924 | FBgn0033378 | tsu | DRSC07265 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00314-A | 35924 | FBgn0033378 | tsu | RNA004085 | 44.11 | Decreased MAPK phosphorylation | | | hit in validation screens | RAS-related MAPK activation | A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila | Ashton-Beaucage et al. | 2014 | 24643257 | Cell line | S2 | MAPK phosphorylation | Fluorescence | Custom-made | Genome-wide | dsRNA | pMAPK signal (%GFP dsRNA) | np | S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai | GR00315-A-1 | 35924 | CG8781 | tsu | 107385 | np | Effect on follicular epithelium morphology | | | further phenotype data in classification screen | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 35924 | CG8781 | tsu | 107385 | np | Defect in formation phase | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | | 35924 | FBgn0033378 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 35924 | CG8781 | | 36025 | np | liquid clearance defect | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 35924 | CG8781 | | 107385 | 1.1540913255252805 | Low decrease in nos and yTub expression | | | Reduced eggs/larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00379-A-2 | 35924 | CG8781 | | 107385 | np | Germarium defects, 100% | | | | Defects in female germline | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | Vasa AND 1B1 expression | Immunofluorescence | VDRC | Selected genes | UAS-IR construct | np | np | Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 35924 | CG8781 | | BL28955 | | none | | | ISC death | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 35924 | CG8781 | | GD36025 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |