RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:36497
  • Symbol:drk
  • Description:downstream of receptor kinase
DataSource: http://genomernai.dkfz.de/v16/genedetails/36497

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00001-A 36497 FBgn0004638 drk DRSC07606 -3.73 Decreased cell size yes Cell size regulation PDGF/VEGF signaling controls cell size in Drosophila. Sims et al. 2009 19216764 Cell line S2R+ F-actin and microtubule protein expression and DNA content Fluorescence rp Genome-wide dsRNA Cell area Z-score < -2
GR00002-A 36497 FBgn0004638 drk DRSC07606 1.009218014 none yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 36497 FBgn0004638 drk DRSC07606 1.545419276 none yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 36497 FBgn0004638 DRSC07606 strong ERK activation reduced no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00026-A-0 36497 FBgn0004638 downstream of receptor kinase np np Morphology no Cell morphology A functional genomic analysis of cell morphology using RNA interference. Kiger et al. 2003 14527345 Cell line Kc167, S2R+ Morphology High content (microscopy) Custom-made dsRNA Visual inspection np
GR00031-A-1 36497 HFA07606 1.7 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 36497 HFA07606 0.7 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
36497 FBgn0013325, FBgn0004638 RpL11, drk LD46519 sp Decreased G1 DNA content yes library: DGC2
36497 FBgn0004638 drk LD12029 sp none no library: DGC1
GR00048-A-2 36497 FBgn0013325, FBgn0004638 RpL11, drk np 0.912 Decreased viability yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Bjӧrklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 36497 FBgn0013325, FBgn0004638 RpL11, drk np 385 Decreased cell number yes Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Bjӧrklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-4 36497 FBgn0013325, FBgn0004638 RpL11, drk np 1.2 Increased number of mitotic cells no Cell size and cell-cycle regulation (4) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Bjӧrklund et al. 2006 16496002 Cell line S2 Number of mitotic cells Fluorescence Selected genes dsRNA Percentage Visual inspection Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00085-A-0 36497 FBgn0004638 CG6033 np np P. aeruginosa invasion decreased no P. aeruginosa infection RNAi screen reveals an Abl kinase-dependent host cell pathway involved in Pseudomonas aeruginosa internalization. Pielage et al. 2008 18369477 Cell line S2 P. aeruginosa infection Invasion assay Custom-made library dsRNA Percent decreased invasion 33%
GR00148-A 36497 FBgn0004638 drk np 2.44 Increased P-JNK protein expression with PGN 60 min PGN induction no PGN-induced dJNK phosphorylation A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. Bond and Foley 2009 19893628 Cell line S2 P-JNK protein expression Fluorescence Custom-made Genome-wide dsRNA Z-score Complex criteria Only hits stored in GenomeRNAi
GR00169-A 36497 FBgn0004638 drk DRSC07606 -2.8 Decreased dAkt phosphorylation after insulin stimulation 10 min insulin stimulation no Akt-TOR pathway negative feedback regulation Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. Kockel et al. 2010 20585550 Cell line Kc167 dAkt phosphorylation Fluorescence Custom-made Genome-wide dsRNA Z-score < -2.5 OR > 2.5
GR00189-A-1 36497 FBgn0004638 drk BKN29233 np Upregulation of Notch pathway no Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00214-A-1 36497 FBgn0004638 drk DRSC07606 -2.64 Decreased ERK phosphorylation after EGF stimulation 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 36497 FBgn0004638 drk DRSC07606 -0.42 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 36497 FBgn0004638 drk DRSC07606 -3.52 Decreased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation yes RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 36497 FBgn0004638 drk DRSC07606 -1.1 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 36497 CG6033 drk AMB33368 2.12 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 36497 CG6033 6033R sp Decreased mannose Chp glycosylation yes Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00239-A-2 36497 CG6033 6033R np none no Glycosylation regulation (2) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation Luminescence np Selected genes UAS-IR construct p-value < 0.005
GR00273-A-1 36497 FBgn0004638 drk DRSC27911 3 Effect on Polycomb (Pc) foci Polycomb group (PcG) proteins regulation (1) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Pc-GFP protein localization Fluorescence DRSC Genome-wide and transcription factors dsRNA Visual inspection 1 Additional phenotypic clustering screen available.
GR00273-A-2 36497 FBgn0004638 drk DRSC27911 np Decreased Polycomb (Pc) foci intensity Polycomb group (PcG) proteins regulation (2) Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen Gonzalez et al. 2014 24703951 Cell line S2 Polycomb-GFP protein localization Fluorescence DRSC Selected genes dsRNA Visual inspection np
GR00282-A 36497 CG6033 drk 105498 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 36497 CG6033 drk 105498 0.07217317747818389 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 36497 FBgn0004638 drk DRSC07606 np Other cellular phenotype decreased cell size Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 36497 CG6033 drk 105498 np Effect on follicular epithelium morphology further phenotype data in classification screen Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00315-A-2 36497 CG6033 drk 105498 np Defect in formation phase Follicular epithelium development (2) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Selected genes UAS-IR construct Visual inspection np For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00326-A 36497 FBgn0004638 drk 6033 np none Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
GR00335-A 36497 FBgn0004638 drk JF02717 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 36497 FBgn0004638 drk JF01377 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
36497 FBgn0004638 np np sp none
GR00367-S 36497 CG6033 105498 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 36497 CG6033 105498 -0.2672914001247154 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A 36497 105498 0.36 none Wing deformity Regulation of olfactory memory formation Identification of genes that promote or inhibit olfactory memory formation in Drosophila. Walkinshaw et al. 2015 25644700 Organism Nsyb-GAL4 T-maze performance Visual inspection VDRC Selected genes UAS-IR construct Performance index, sp High performer in olfactory memory formation: > Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A 36497 CG6033 BL27563 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 36497 CG6033 KK105498 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.