| GR00002-A | 36571 | FBgn0000289 | cg | DRSC07650 | -0.56248406 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 36571 | FBgn0000289 | cg | DRSC07650 | 0.06699923 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 36571 | FBgn0000289 | cg | DRSC23142 | -1.353155576 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 36571 | FBgn0000289 | cg | DRSC23142 | -0.606383673 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00028-A-0 | 36571 | FBgn0000289 | CG8367 | np | np | Decreased Hedgehog reporter activity | | no | | Hedgehog signaling | Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells. | Lum et al. | 2003 | 12663920 | Cell line | Clone 8 | Hedgehog signaling | Dual luciferase | Custom-made | | dsRNA | Hedgehog reporter activity | np | | GR00031-A-1 | 36571 | | mig | HFA07650 | 6 | Decreased cell number and viability | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 36571 | | mig | HFA07650 | 5.1 | Decreased cell number and viability | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00045-A-0 | 36571 | FBgn0000289 | | DRSC07650 | -4.51 | Decreased Hedgehog reporter activity | | no | | Hedgehog signaling | A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. | Nybakken et al. | 2005 | 16311596 | Cell line | Clone 8 | Hedgehog signaling | Dual luciferase | DRSC | | dsRNA | Z-score | > | | GR00047-A-1 | 36571 | FBgn0000289 | cg | DRSC07650 | sp | Decreased horseradish peroxidase protein expression | | no | | Constitutive protein secretion and Golgi organization (1) | Functional genomics reveals genes involved in protein secretion and Golgi organization. | Bard et al. | 2006 | 16452979 | Cell line | S2 | Horseradish peroxidase protein expression | Luminescence | | Genome-wide | dsRNA | Z-score | < -1.5 | | | 36571 | FBgn0000289 | cg | LD21089 | sp | Decreased G1 DNA content, decreased G2 DNA content, increased cytokinesis | | yes | library: DGC1 | | | | | | | | | | | | | | | | | 36571 | FBgn0000289 | cg | np | sp | none | | yes | library: DGC1 | | | | | | | | | | | | | | | | | 36571 | FBgn0000289 | cg | GH24215 | sp | none | | yes | library: DGC1 | | | | | | | | | | | | | | | | GR00048-A-2 | 36571 | FBgn0000289 | cg | np | 1.075 | none | | yes | | Cell size and cell-cycle regulation (2) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Proliferation and viability | Colorimetrics | | Selected genes | dsRNA | MTS metabolic activity | > 2.5 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-3 | 36571 | FBgn0000289 | cg | np | 483 | none | | yes | | Cell size and cell-cycle regulation (3) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Cell number | Fluorescence | | Selected genes | dsRNA | Average cell number per field | > 2 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-4 | 36571 | FBgn0000289 | cg | np | below threshold | none | | no | low cell number | Cell size and cell-cycle regulation (4) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Number of mitotic cells | Fluorescence | | Selected genes | dsRNA | Percentage | Visual inspection | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | | 36571 | CG8367 | cg | np | np | Heart patterning defects | | no | | | | | | | | | | | | | | | | | GR00065-A | 36571 | CG8367 | cg | cg | np | none | | no | | Dendrite pattern formation | Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. | Parrish et al. | 2006 | 16547170 | Tissue | GAL4221 | mCD8 protein expression | Fluorescence | Custom-made | Transcription factors | UAS-IR construct | np | Phenotypes in multiple blind tests | Additional information about secondary screens | GR00128-A-1 | 36571 | FBgn0000289 | CG8367 | DRSC07650 | sp | none | | no | non-significantly decreased viability,hit according to not so stringent primary analysis | Combinatorial effect with methyl methanesulphonate (1) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Genome-wide | dsRNA | rp | rp | Additional information about the primary screen | GR00128-A-2 | 36571 | FBgn0000289 | CG8367 | DRSC32302 | sp | none | | no | | Combinatorial effect with methyl methanesulphonate (2) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Selected genes | dsRNA | rp | rp | | GR00135-A-1 | 36571 | CG8367 | cg | 2965 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 36571 | CG8367 | cg | 2966 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00142-A-1 | 36571 | CG8367 | cg | 2965 | -2.61818181818181 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 36571 | CG8367 | | 2965 | sp | Completely lethal (before pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 36571 | CG8367 | | 2966 | sp | Completely lethal (before pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00170-A-1 | 36571 | | combgap | DRSC07650 | -6.1 | none | | no | decreased cell viability or Sanger collection sequence | HIF-dependent transcription (1) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 1.0 library | Genome-wide | dsRNA | Z-score | < | | GR00175-A | 36571 | CG8367 | cg | DRSC32302 | -0.723731972894895 | Downregulation of Hh pathway | | no | | Hh pathway regulation | A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. | DasGupta et al. | 2007 | 17903264 | Cell line | rp | rp | Luminescence | DRSC-v | Selected genes | dsRNA | Average fractional change | Complex criteria | | GR00175-A | 36571 | CG8367 | cg | DRSC32301 | -0.750864878239531 | Downregulation of Hh pathway | | no | | Hh pathway regulation | A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. | DasGupta et al. | 2007 | 17903264 | Cell line | rp | rp | Luminescence | DRSC-v | Selected genes | dsRNA | Average fractional change | Complex criteria | | GR00218-S | 36571 | CG8367 | | AMB20617 | 0.39 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00282-A | 36571 | CG8367 | cg | 2965 | np | none | | | rudimentary ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 36571 | CG8367 | cg | 2966 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 36571 | CG8367 | cg | 2966 | 0.16437029701462574 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00291-A | 36571 | | cg | DRSC07650 | sp | none | | | eliminated based on control score | Hedgehog (hh) secretion regulation | A Genome-Wide RNAi Screen Identifies Regulators of Cholesterol-Modified Hedgehog Secretion in Drosophila | Aikin et al. | 2012 | 22432040 | Cell line | S2 | Hedgehog protein expression | Luminescence | DRSC | Genome-wide | dsRNA | Z-score | < -2 OR > 2 by at least 1/3 Z-scores | 296 hits were eliminated by the authors for several criteria, sp. Reagents targeting unknown genes (HDCs) are not displayed. Additional secondary screen available. | GR00311-A | 36571 | np | np | DRSC07650 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 36571 | CG8367 | cg | 102054 | np | none | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00335-A | 36571 | FBgn0000289 | cg | JF02523 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 36571 | FBgn0000289 | cg | HMS01145 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 36571 | np | np | HFA07650 | 0.8 | none | | | fold change: 0.53 | | | | | | | | | | | | | | | | | 36571 | FBgn0000289 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00379-A-1 | 36571 | CG8367 | | 2966 | -0.44925547839368996 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 36571 | CG8367 | | BL34668 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 36571 | CG8367 | | BL29359 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 36571 | CG8367 | | GD2966 | | Lethal | | | ISC death | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |