| GR00002-A | 36682 | FBgn0033995 | CG8090 | DRSC07075 | -0.251268358 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 36682 | FBgn0033995 | CG8090 | DRSC07075 | 0.313229778 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00012-A-1 | 36682 | CG8090 | dronc | np | np | Increased viability with doxorubicin | | yes | | Caspase activation in apoptosis (1) - combinatorial effect with doxorubicin | A genome-wide RNAi screen reveals multiple regulators of caspase activation. | Yi et al. | 2007 | 17998402 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | HFA | Genome-wide | dsRNA | Z-score | Complex criteria | | GR00012-A-2 | 36682 | CG8090 | dronc | np | np | Decreased caspase-3/7-like activity with doxorubicin | | yes | | Caspase activation in apoptosis (2) - combinatorial effect with doxorubicin | A genome-wide RNAi screen reveals multiple regulators of caspase activation. | Yi et al. | 2007 | 17998402 | Cell line | Kc167 | Caspase 3/7 activity | Luminescence | HFA | Selected genes | dsRNA | p-value | < 0.05 | | GR00012-A-3 | 36682 | CG12284, CG8090 | diap1, dronc | np | np | Increased viability with diap1 RNAi | | no | | Caspase activation in apoptosis (3) - combinatorial effect with diap1 RNAi | A genome-wide RNAi screen reveals multiple regulators of caspase activation. | Yi et al. | 2007 | 17998402 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | HFA | Selected genes | dsRNA | p-value | < 0.05 | | GR00031-A-1 | 36682 | | | HFA07075 | 0 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 36682 | | | HFA07075 | -0.7 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 36682 | FBgn0033995 | CG8090 | LD13915 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | GR00134-A-1 | 36682 | CG8090 | CG8090 | 30339 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 36682 | CG8090 | CG8090 | 30341 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 36682 | CG8090 | CG8090 | 30339 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 36682 | CG8090 | CG8090 | 30341 | -0.31 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 36682 | CG8090 | | 30341 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 36682 | CG8090 | CG8090 | 30341 | 0.170731707317073 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 36682 | CG8090 | | 30339 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 36682 | CG8090 | | 30341 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00190-A-1 | 36682 | FBgn0033995 | CG8090 | 30341 | -0.104 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00191-A-1 | 36682 | CG8090 | CG8090 | np | np | none | | yes | | Blood cell homeostasis regulation (1) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-2 | 36682 | CG8090 | CG8090 | np | np | none | | no | | Blood cell homeostasis regulation (2) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00217-A-1 | 36682 | CG8090 | CG8090 | np | strong | Decreased WgRluc and not changed sFluc protein expression | | yes | | Wg pathway regulation (1) | A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. | Port et al. | 2011 | 21886182 | Cell line | S2R+ | WgRluc and sFluc protein expression | Luminescence | np | Genome-wide | dsRNA | Phenotype strength | Complex criteria | Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4) | GR00217-A-2 | 36682 | CG8090 | CG8090 | 100602 | 1 | Enhanced sev–wg rough-eye phenotype | | yes | | Wg pathway regulation (2) | A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. | Port et al. | 2011 | 21886182 | Tissue | ey-GAL4 GMR–GAL4 | Viability and Wg protein expression | Visual inspection and eye morphology | KK | Selected genes | UAS-IR construct | Phenotype strength | np | Phenotype strength: strong enhancement: 2; strong suppression: -2; unchanged: 0; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4) | GR00217-A-3 | 36682 | CG8090 | CG8090 | 100602 | wildtype | none | | yes | | Wg pathway regulation (3) | A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. | Port et al. | 2011 | 21886182 | Tissue | nub-GAL4 | Viability and wing morphology | Visual inspection | KK | Selected genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4) | GR00217-A-4 | 36682 | CG8090 | CG8090 | 100602 | wildtype | none | | no | | Wg pathway regulation (4) | A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. | Port et al. | 2011 | 21886182 | Tissue | hhGAL4 | Viability and wing morphology | Visual inspection | KK and np | Selected genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4) | GR00218-S | 36682 | CG8090 | | AMB20550 | -0.66 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 36682 | CG8090 | | 8090R | sp | Decreased alpha1,6-fucose Chp glycosylation, decreased (GlcNAc)n Chp glycosylation, decreased GlcNAcb1-4GlcNAc Chp glycosylation | | yes | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00239-A-2 | 36682 | CG8090 | | 8090R | np | none | | no | | Glycosylation regulation (2) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation | Luminescence | np | Selected genes | UAS-IR construct | p-value | < 0.005 | | GR00282-A | 36682 | CG8090 | CG8090 | 30339 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 36682 | CG8090 | CG8090 | 30341 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 36682 | CG8090 | CG8090 | 100602 | 0.6738250022857596 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 36682 | FBgn0033995 | CG8090 | DRSC07075 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 36682 | CG8090 | CG8090 | 100602 | np | Effect on follicular epithelium morphology | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 36682 | CG8090 | CG8090 | 100602 | np | none | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | | 36682 | FBgn0033995 | np | np | sp | none | | | | | | | | | | | | | | | | | | | | 36682 | np | np | HFA07075 | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 36682 | CG8090 | | 100602 | np | none | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 36682 | CG8090 | | 100602 | -0.2001768198646452 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00385-A | 36682 | | | 100602 | 0.23 | Low performer in olfactory memory formation | | | | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00388-A | 36682 | CG8090 | | KK100602 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |