GR00001-A | 36740 | FBgn0083919 | CG30084 | DRSC07121 | -2.16 | none | | no | low cell number | Cell size regulation | PDGF/VEGF signaling controls cell size in Drosophila. | Sims et al. | 2009 | 19216764 | Cell line | S2R+ | F-actin and microtubule protein expression and DNA content | Fluorescence | rp | Genome-wide | dsRNA | Cell area Z-score | < -2 | | GR00002-A | 36740 | FBgn0034046 | tun | DRSC06269 | 1.047083549 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 36740 | FBgn0034046 | tun | DRSC06269 | 0.007659052 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 36740 | FBgn0034046 | tun | DRSC07121 | 0.495723138 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 36740 | FBgn0034046 | tun | DRSC07121 | -1.632982943 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00021-A-0 | 36740 | FBgn0083919 | | DRSC06269 | weak | ERK activation reduced | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 36740 | | | HFA07121 | -0.2 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-1 | 36740 | | | HFA06269 | 0.2 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 36740 | | | HFA07121 | 0.2 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 36740 | | | HFA06269 | 1.4 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00047-A-1 | 36740 | FBgn0050084 | tun | DRSC06269 | sp | Decreased horseradish peroxidase protein expression | | yes | | Constitutive protein secretion and Golgi organization (1) | Functional genomics reveals genes involved in protein secretion and Golgi organization. | Bard et al. | 2006 | 16452979 | Cell line | S2 | Horseradish peroxidase protein expression | Luminescence | | Genome-wide | dsRNA | Z-score | < -1.5 | | GR00047-A-2 | 36740 | FBgn0050084 | tun | np | np | Fragmented Golgi membranes | | no | | Constitutive protein secretion and Golgi organization (2) | Functional genomics reveals genes involved in protein secretion and Golgi organization. | Bard et al. | 2006 | 16452979 | Cell line | S2 | Mouse Mannosidase II protein expression | Fluorescence | | Selected genes | dsRNA | Visual inspection | Golgi membrane depletion > 50 % of the cells | | | 36740 | FBgn0050084 | tun | np | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 36740 | FBgn0050084 | tun | LP02021 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 36740 | FBgn0050084 | tun | GH15137 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | | 36740 | FBgn0050084, FBgn0002940 | tun, ninaE | GH27957 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | GR00064-A | 36740 | | CG12969 | np | 8.6 | Downregulation of RNAi pathway | | | | RNAi pathway regulation | A genomewide screen for components of the RNAi pathway in Drosophila cultured cells | Dorner et al. | 2006 | 16882716 | Cell line | SR2+ | RNAi pathway reporter | Luminescence | HFA | Selected genes | dsRNA | Z-score | > 4 | See comment for final hits. HFA sequences provided. | GR00071-A-0 | 36740 | FBgn0083919 | CG30084 | np | np | Processes | | no | | Integrin adhesion | Zasp is required for the assembly of functional integrin adhesion sites. | Jani et al. | 2007 | 18166658 | Cell line | S2, S2R+ | Cell morphology (actin) | High content (microscopy) | Custom-made library | | dsRNA | Visual inspection | np | | GR00130-A | 36740 | FBgn0083919 | CG30084 | np | <= -2 | Decreased mutant human huntingtin aggregation | | no | | Mutant human huntingtin aggregation | RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. | Doumanis et al. | 2009 | 19789644 | Cell line | BG2-c2 | Nhtt(62Q)EGFP aggregate number and size | Fluorescence | OpenBiosystems RNAi library | Selected genes | dsRNA | Z-score | Suppressor: < | | GR00134-A-1 | 36740 | CG30084 | Zasp | 36564 | np | none | | yes | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 36740 | CG30084 | Zasp | 36563 | np | Semi-lethal | | yes | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-2 | 36740 | CG30084 | Zasp | np | np | Wing posture / locomotion / ability to fly defect or lethal / semi-lethal | | no | | Muscle morphogenesis and function (2) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | Custom-made | Selected genes | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 36740 | CG30084 | CG30084 | 36563 | 1.13 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 36740 | CG30084 | CG30084 | 36564 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00142-A-1 | 36740 | CG30084 | CG30084 | 36564 | 0.183098591549295 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 36740 | CG30084 | | 36563 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 36740 | CG30084 | | 36564 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00148-A | 36740 | FBgn0050084 | tun | np | 2.58 | Increased P-JNK protein expression with PGN | 15 min PGN induction | no | | PGN-induced dJNK phosphorylation | A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. | Bond and Foley | 2009 | 19893628 | Cell line | S2 | P-JNK protein expression | Fluorescence | Custom-made | Genome-wide | dsRNA | Z-score | Complex criteria | Only hits stored in GenomeRNAi | GR00190-A-1 | 36740 | FBgn0083919 | CG30084 | 36564 | 0.063 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00214-A-1 | 36740 | FBgn0083919 | Zasp52 | DRSC06269 | -1.25 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 36740 | FBgn0083919 | Zasp52 | DRSC06269 | -0.05 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 36740 | FBgn0083919 | Zasp52 | DRSC06269 | 1.12 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 36740 | FBgn0083919 | Zasp52 | DRSC06269 | 1.25 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 36740 | CG30084 | Zasp52 | AMB34618 | 0.96 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00223-A | 36740 | | Zasp | DRSC07121 | <-2 | Decreased intracellular Francisella tularensis infection | | yes | | Intracellular Francisella tularensis infection | Host factors required for modulation of phagosome biogenesis and proliferation of Francisella tularensis within the cytosol. | Akimana et al. | 2010 | 20552012 | Cell line | S2R+ | GFP protein expression | Fluorescence | np | Selected genes | dsRNA | Z-score | Strong: >3 OR <-2; medium: >2 OR <-1.2; weak: <-0.75 | Additional information about the primary genome-wide screen | GR00227-A | 36740 | CG30084 | Zasp | HFA06269 | 2.72 | Upregulation of IMD pathway after E.coli stimulation | | no | | Immune deficiency pathway regulation | Drosophila Ras/MAPK signalling regulates innate immune responses in immune and intestinal stem cells. | Ragab et al. | 2011 | 21297578 | Cell line | SL2 | Immune deficiency pathway reporter | Luminescence | HFA | Genome-wide | dsRNA | Z-score | np | | GR00282-A | 36740 | CG30084 | Zasp52 | 36563 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 36740 | CG30084 | Zasp52 | 36564 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 36740 | CG30084 | Zasp52 | 106177 | 0.4259163806286103 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00308-A | 36740 | CG8242 | | np | 1.67 | Decreased cell aggregation | | | | Cadherin-mediated cell-cell adhesion | A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion | Toret et al. | 2014 | 24446484 | Cell line | S2 | Cell aggregation | Fluorescence | V2 RNAi library (Thermo Fisher Scientific) | Genome-wide | dsRNA | Visual inspection; average of 3 replicates; 0 | > 1.5 | Additional secondary screen available. S2 cells stably express DE-cadherin. | GR00311-A | 36740 | FBgn0083919 | Zasp52 | DRSC07121 | np | Other cellular phenotype | | | decreased cell size | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00311-A | 36740 | FBgn0083919 | Zasp52 | DRSC06269 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00314-A | 36740 | FBgn0083919 | Zasp52 | RNA003773 | 133.15 | Increased MAPK phosphorylation | | | | RAS-related MAPK activation | A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila | Ashton-Beaucage et al. | 2014 | 24643257 | Cell line | S2 | MAPK phosphorylation | Fluorescence | Custom-made | Genome-wide | dsRNA | pMAPK signal (%GFP dsRNA) | np | S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai | GR00315-A-1 | 36740 | CG30084 | Zasp52 | 106177 | np | none | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00326-A | 36740 | FBgn0083919 | Zasp52 | 30084 | np | none | | | | Synapse formation and maintenance | A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance | Valakh et al. | 2012 | 22542760 | Tissue | Elav-Gal4 | Neuromuscular junction morphology | Fluorescence | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | For further phenotype explanations sp. | GR00335-A | 36740 | FBgn0083919 | Zasp52 | JF01133 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 36740 | FBgn0083919 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 36740 | CG30084 | | 36563 | np | lethal or adult morphology phenotype | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 36740 | CG30084 | | 106177 | -0.1835261957678773 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 36740 | CG30084 | | GD36564 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 36740 | CG30084 | | GD36563 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |