RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:38017
  • Symbol:Pdk1
  • Description:Phosphoinositide-dependent kinase 1
DataSource: http://genomernai.dkfz.de/v16/genedetails/38017

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00001-A 38017 FBgn0020386 Pk61C DRSC08682 -1.13 none yes Cell size regulation PDGF/VEGF signaling controls cell size in Drosophila. Sims et al. 2009 19216764 Cell line S2R+ F-actin and microtubule protein expression and DNA content Fluorescence rp Genome-wide dsRNA Cell area Z-score < -2
GR00002-A 38017 FBgn0020386 Pk61C DRSC08682 2.372550174 Increased lipid droplet area yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 38017 FBgn0020386 Pk61C DRSC08682 6.439134592 Increased lipid droplet area yes Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 38017 FBgn0020386 DRSC08682 strong ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00030-A-1 38017 CG1210 Pk61C Pk61C, Pk61C_2 sp Increased G1 DNA content, decreased cell size, decreased mitotic index yes Cell cycle regulation (1) Genome-wide survey of protein kinases required for cell cycle progression. Bettencourt-Dias et al. 2004 15616552 Cell line S2 alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content Flow cytometry rp Kinases dsRNA Confidence intervals Complex criteria
GR00030-A-2 38017 CG1210 Pk61C Pk61C, Pk61C_2 np Increased G1 DNA content, decreased cell size no Cell cycle regulation (2) Genome-wide survey of protein kinases required for cell cycle progression. Bettencourt-Dias et al. 2004 15616552 Cell line S2 Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression Flow cytometry rp Kinases and kinase regulators dsRNA np np
GR00031-A-1 38017 HFA08682 -1.2 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 38017 HFA08682 -0.2 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
38017 FBgn0020386 Pk61C LD22131 sp none yes library: DGC1
38017 FBgn0020386 Pk61C np sp Increased G1 DNA content yes library: PHOSPHO
38017 FBgn0020386 Pk61C CT1275 sp none yes library: PHOSPHO
38017 FBgn0020386 Pk61C CT2222 sp none yes library: PHOSPHO
38017 FBgn0020386 Pk61C CT42507 sp none yes library: PHOSPHO
38017 FBgn0020386 Pk61C CT42509 sp none yes library: PHOSPHO
38017 FBgn0020386 Pk61C CT42511 sp none yes library: PHOSPHO
38017 FBgn0020386 Pk61C CT42513 sp none yes library: PHOSPHO
38017 FBgn0052306, FBgn0020386 CG32306, Pk61C AT07287 sp Increased G1 DNA content yes library: DGC2
38017 FBgn0050182, FBgn0020386 CG30182, Pk61C RH07911 sp none no library: DGC2
GR00048-A-2 38017 FBgn0052306, FBgn0020386 CG32306, Pk61C np 1.193 none yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Bjӧrklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-2 38017 FBgn0020386 Pk61C np 1.321 none yes Cell size and cell-cycle regulation (2) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Bjӧrklund et al. 2006 16496002 Cell line S2 Proliferation and viability Colorimetrics Selected genes dsRNA MTS metabolic activity > 2.5 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 38017 FBgn0020386 Pk61C np 452 none no Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Bjӧrklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00048-A-3 38017 FBgn0052306, FBgn0020386 CG32306, Pk61C np 435 none no Cell size and cell-cycle regulation (3) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Bjӧrklund et al. 2006 16496002 Cell line S2 Cell number Fluorescence Selected genes dsRNA Average cell number per field > 2 standard deviations Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells)
GR00089-A 38017 CG1210 np 0.001 Decreased dFoxO reporter expression FoxO regulation RNAi screening for kinases and phosphatases identifies FoxO regulators Mattila et al. 2008 18815370 Cell line S2 dFoxO reporter expression Fluorescence Custom-made Kinases and phosphatases dsRNA P-value < Genome RNAi mapping in column after comment (FBgn)
GR00090-A-0 38017 FBgn0020386 CG1210 np automated analysis Altered mean signal no Lipid storage Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Guo et al. 2008 18408709 Cell line S2 Lipid droplet staining High content (microscopy) OpenBiosystems dsRNA Visual inspection np
GR00128-A-2 38017 FBgn0020386 CG1210 DRSC30826 sp Synthetic lethal with methyl methanesulphonate no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00134-A-1 38017 CG1210 Pk61C 18736 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 38017 CG1210 Pk61C 18736 -0.14 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 38017 CG1210 18736 1 none yes Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00138-A-2 38017 CG1210 18736 5.45 Decreased viability no Heart development and function (2) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Z-score > 3
GR00142-A-1 38017 CG1210 Pk61C 18736 0.929577464788732 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 38017 CG1210 18736 0.6 Notum malformation migration no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00169-A 38017 FBgn0020386 Pk61C DRSC08682 2.5 none 10 min insulin stimulation no Akt-TOR pathway negative feedback regulation Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. Kockel et al. 2010 20585550 Cell line Kc167 dAkt phosphorylation Fluorescence Custom-made Genome-wide dsRNA Z-score < -2.5 OR > 2.5
GR00169-A 38017 FBgn0020386 Pk61C DRSC08682 4.8 Increased dAkt phosphorylation without insulin stimulation no Akt-TOR pathway negative feedback regulation Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. Kockel et al. 2010 20585550 Cell line Kc167 dAkt phosphorylation Fluorescence Custom-made Genome-wide dsRNA Z-score < -2.5 OR > 2.5
GR00170-A-1 38017 PDK1 DRSC08682 -2.3 Decreased HIF dependent transcription with desferrioxamine yes HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00170-A-2 38017 PDK1 DRSC30826 36.1 Decreased HIF dependent transcription with desferrioxamine no group B HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-2 38017 PDK1 DRSC30825 51 Decreased HIF dependent transcription with desferrioxamine no group B HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-3 38017 dPDK1 DRSC30826 56.2 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00170-A-3 38017 dPDK1 DRSC30825 51.5 Decreased HIF-dependent transcription under hypoxia no HIF-dependent transcription (3) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage >
GR00190-A-1 38017 FBgn0020386 Pk61C 18736 -0.327 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00201-A-1 38017 FBgn0020386 Pk61C CG1210 (UCSF), Pk61C (Baum_Lab) np none yes Actin organization and cell morphology (1) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line S2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-2 38017 FBgn0020386 Pk61C CG1210 (UCSF), Pk61C (Baum_Lab) np none yes Actin organization and cell morphology (2) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line S2R+ alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-3 38017 FBgn0020386 Pk61C CG1210 (UCSF), Pk61C (Baum_Lab) np none yes Actin organization and cell morphology (3) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line Kc167 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-4 38017 FBgn0020386 Pk61C CG1210 (UCSF), Pk61C (Baum_Lab) np none yes Actin organization and cell morphology (4) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG2-c2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-5 38017 FBgn0020386 Pk61C CG1210 (UCSF), Pk61C (Baum_Lab) np none yes Actin organization and cell morphology (5) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG3-c1 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00201-A-6 38017 FBgn0020386 Pk61C CG1210 (UCSF), Pk61C (Baum_Lab) np none no Actin organization and cell morphology (6) Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. Liu et al. 2009 19265526 Cell line BG3-c2 alpha-Tubulin and F-actin protein expression Fluorescence Custom-made Kinases dsRNA Visual inspection np
GR00202-A 38017 FBgn0020386 Pk61C np sp none no Vaccinia virus infection A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. Moser et al. 2010 20585561 Cell line DL1 Virally encoded beta-galactosidase protein expression Fluorescence Custom-made Kinases, phosphatases and selected genes dsRNA Z-score < -2 in both duplicates Author-reviewed data
GR00214-A-1 38017 FBgn0020386 Pk61C DRSC08682 -1.29 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 38017 FBgn0020386 Pk61C DRSC08682 -1.88 Decreased ERK phosphorylation after EGF stimulation 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 38017 FBgn0020386 Pk61C DRSC08682 1.81 Increased ERK phosphorylation after sSpitz and EGF stimulation 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 38017 FBgn0020386 Pk61C DRSC08682 0.23 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00217-A-1 38017 CG1210 Pk61C np medium Decreased WgRluc and not changed sFluc protein expression yes Wg pathway regulation (1) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Cell line S2R+ WgRluc and sFluc protein expression Luminescence np Genome-wide dsRNA Phenotype strength Complex criteria Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-2 38017 CG1210 Pk61C 18736 -1 Suppressed sev–wg rough-eye phenotype yes Wg pathway regulation (2) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue ey-GAL4 GMR–GAL4 Viability and Wg protein expression Visual inspection and eye morphology KK Selected genes UAS-IR construct Phenotype strength np Phenotype strength: strong enhancement: 2; strong suppression: -2; unchanged: 0; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-3 38017 CG1210 Pk61C 18736 wildtype none yes Wg pathway regulation (3) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue nub-GAL4 Viability and wing morphology Visual inspection KK Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00217-A-4 38017 CG1210 Pk61C 18736 weak Wings: P compartment smaller no Wg pathway regulation (4) A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. Port et al. 2011 21886182 Tissue hhGAL4 Viability and wing morphology Visual inspection KK and np Selected genes UAS-IR construct Phenotype strength np Additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4)
GR00218-S 38017 CG1210 Pdk1 AMB22211 1.1 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00220-A 38017 FBgn0020386 Pk61C BKN28363 -5.19 Decreased Srp/Lz-induced transcription yes Srp/Lz-induced transcriptional activation A genome-wide RNA interference screen identifies a differential role of the mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila. Gobert et al. 2010 20368357 Cell line Kc167 Srp/Lz-induced transcription reporter (PO45) Luminescence BKN Genome-wide dsRNA Z-score > 4 OR < -4
GR00282-A 38017 CG1210 Pdk1 18736 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 38017 CG1210 Pdk1 109812 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 38017 CG1210 Pdk1 109812 -1.0326367740973474 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 38017 FBgn0020386 Pk61C DRSC08682 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00326-A 38017 FBgn0020386 Pdk1 1210 np none Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
GR00326-A 38017 FBgn0020386 Pk61C 109812 np Synaptic retractions Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
GR00335-A 38017 FBgn0020386 Pdk1 HMS01250 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 38017 FBgn0020386 Pdk1 GL00489 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 38017 FBgn0020386 Pdk1 JF02807 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
38017 FBgn0020386 np np sp none
GR00367-S 38017 CG1210 18736 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00370-A 38017 FBgn0020386 Pk61C -0.18 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00370-A 38017 FBgn0020386 Pk61C 0.73 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00370-A 38017 FBgn0020386 Pk61C 0.87 none Regulation of Nrf2 homolog CncC Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes Li et al. 2016 26911346 Cell line S2 CncC reporter Luminescence HD2 Kinases dsRNA Z-Score <-1.65 OR >1.65
GR00379-A-1 38017 CG1210 109812 3.448821764660549 Medium decrease in nos and yTub37c expression Reduced eggs/larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2 38017 CG1210 109812 np No germline in 30%; Germarium defects in 70% Defects in female germline Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 Vasa AND 1B1 expression Immunofluorescence VDRC Selected genes UAS-IR construct np np Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 38017 CG1210 BL27725 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 38017 CG1210 BL34936 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.