| GR00002-A | 38516 | FBgn0041171 | ago | DRSC08463 | 1.100183445 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 38516 | FBgn0041171 | ago | DRSC08463 | 0.876590555 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00004-A-0 | 38516 | | ago | DRSC08463 | 1.04 | siRNA reporter downregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00004-A-0 | 38516 | | ago | DRSC08463 | 2.92 | miRNA reporter upregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00021-A-0 | 38516 | FBgn0041171 | | DRSC08463 | moderate | ERK activation reduced | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 38516 | | | HFA08463 | -2.1 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 38516 | | | HFA08463 | 0 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 38516 | FBgn0041171 | ago | LD30271 | sp | Decreased G1 cell size | | yes | library: DGC1 | | | | | | | | | | | | | | | | | 38516 | FBgn0041171 | ago | LD21322 | sp | none | | yes | library: DGC1 | | | | | | | | | | | | | | | | | 38516 | FBgn0041171 | ago | np | sp | none | | yes | library: DGC1 | | | | | | | | | | | | | | | | GR00048-A-2 | 38516 | FBgn0041171 | ago | np | 0.972 | none | | yes | | Cell size and cell-cycle regulation (2) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | BjÓ§rklund et al. | 2006 | 16496002 | Cell line | S2 | Proliferation and viability | Colorimetrics | | Selected genes | dsRNA | MTS metabolic activity | > 2.5 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-3 | 38516 | FBgn0041171 | ago | np | 456 | none | | no | | Cell size and cell-cycle regulation (3) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | BjÓ§rklund et al. | 2006 | 16496002 | Cell line | S2 | Cell number | Fluorescence | | Selected genes | dsRNA | Average cell number per field | > 2 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00066-A | 38516 | CG15010 | archipelago | np | np | Lethal | | no | lethal > 90 %, hatching rate: 7 % | Cellularisation regulation | Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation. | Pilot et al. | 2006 | 16421189 | Organism | OreR and yw | Cellularisation, early gastrulation and viability | Microscopy | Heidelberg GenomeRNAi Drosophila resources | Selected genes | UAS-IR construct | Percentage penetrance | np | | GR00128-A-2 | 38516 | FBgn0041171 | CG15010 | DRSC30818 | sp | none | | no | | Combinatorial effect with methyl methanesulphonate (2) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Selected genes | dsRNA | rp | rp | | GR00134-A-1 | 38516 | CG15010 | ago | 4281 | np | Flightless | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 38516 | CG15010 | ago | 4282 | np | Flightless | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00159-A | 38516 | | ago | DRSC08463 | np | none | | no | | Muscle assembly and maintenance | RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. | Bai et al. | 2008 | 18359903 | Primary cells | primary embryonic cells | Muscle cell morphology | Fluorescence | np | Selected and random genes | dsRNA | Percentage of muscles with a given phenotype | Severe: > 80 %; medium: ~ 50 % | | GR00214-A-1 | 38516 | FBgn0041171 | ago | DRSC08463 | 1.47 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 38516 | FBgn0041171 | ago | DRSC08463 | 1.47 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 38516 | FBgn0041171 | ago | DRSC08463 | 0.13 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 38516 | FBgn0041171 | ago | DRSC08463 | -0.53 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 38516 | CG15010 | | AMB26104 | 0.62 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 38516 | CG15010 | | 15010R | sp | Decreased (GlcNAc)n Chp glycosylation | | yes | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00239-A-2 | 38516 | CG15010 | | 15010R | np | none | | no | | Glycosylation regulation (2) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation | Luminescence | np | Selected genes | UAS-IR construct | p-value | < 0.005 | | GR00311-A | 38516 | FBgn0041171 | ago | DRSC08463 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00335-A | 38516 | FBgn0041171 | ago | HM04005 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 38516 | FBgn0041171 | ago | HMS00111 | np | Embryonic lethal | | | many embryonic lethal | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 38516 | FBgn0041171 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00388-A | 38516 | CG15010 | | BL34802 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |