| GR00002-A | 38605 | FBgn0052423 | CG32423 | DRSC09765 | -0.028759521 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 38605 | FBgn0052423 | CG32423 | DRSC09765 | 0.00367131 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 38605 | FBgn0052423 | CG32423 | DRSC09771 | -0.837400191 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 38605 | FBgn0052423 | CG32423 | DRSC09771 | 0.673441021 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00031-A-1 | 38605 | | | HFA08748 | 1.2 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-1 | 38605 | | | HFA09765 | -0.2 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-1 | 38605 | | | HFA09771 | -0.7 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 38605 | | | HFA09771 | -1.1 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 38605 | | | HFA08748 | 4 | Decreased cell number and viability | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 38605 | | | HFA09765 | -0.3 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00035-A | 38605 | CG32423 | CG32423 | JP028 | sp | none | | no | | Alternative splicing | Identification of alternative splicing regulators by RNA interference in Drosophila. | Park et al. | 2004 | 15492211 | Cell line | S2 | dAdar, para and Dscam mRNA expression | Reverse transcription PCR and autoradiography | Custom-made | RNA-binding protein genes and spliceosomal genes | dsRNA | np | Alternative exon splicing for > | | GR00047-A-1 | 38605 | FBgn0052423 | CG32423 | DRSC08748 | sp | Decreased horseradish peroxidase protein expression | | no | | Constitutive protein secretion and Golgi organization (1) | Functional genomics reveals genes involved in protein secretion and Golgi organization. | Bard et al. | 2006 | 16452979 | Cell line | S2 | Horseradish peroxidase protein expression | Luminescence | | Genome-wide | dsRNA | Z-score | < -1.5 | | | 38605 | FBgn0035596, FBgn0052423 | CG10677, CG32423 | RH63980 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | | 38605 | FBgn0052423 | CG32423 | LD40028 | sp | Decreased G1 cell size | | yes | library: DGC2 | | | | | | | | | | | | | | | | | 38605 | FBgn0052423 | CG32423 | GM08769 | sp | Increased G1 DNA content | | yes | library: DGC1 | | | | | | | | | | | | | | | | | 38605 | FBgn0052423 | CG32423 | np | sp | none | | yes | library: DGC1 | | | | | | | | | | | | | | | | | 38605 | FBgn0052423 | CG32423 | HL01227 | sp | none | | yes | library: DGC1 | | | | | | | | | | | | | | | | GR00048-A-2 | 38605 | FBgn0052423 | CG32423 | np | 1.175 | none | | yes | | Cell size and cell-cycle regulation (2) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Proliferation and viability | Colorimetrics | | Selected genes | dsRNA | MTS metabolic activity | > 2.5 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-2 | 38605 | FBgn0052423 | CG32423 | np | 1.323 | none | | yes | | Cell size and cell-cycle regulation (2) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Proliferation and viability | Colorimetrics | | Selected genes | dsRNA | MTS metabolic activity | > 2.5 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-3 | 38605 | FBgn0052423 | CG32423 | np | 450 | none | | no | | Cell size and cell-cycle regulation (3) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Cell number | Fluorescence | | Selected genes | dsRNA | Average cell number per field | > 2 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-3 | 38605 | FBgn0052423 | CG32423 | np | 468 | none | | no | | Cell size and cell-cycle regulation (3) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Cell number | Fluorescence | | Selected genes | dsRNA | Average cell number per field | > 2 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00128-A-1 | 38605 | FBgn0052423 | CG32423 | DRSC09771 | sp | none | | no | hit according to not so stringent primary analysis | Combinatorial effect with methyl methanesulphonate (1) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Genome-wide | dsRNA | rp | rp | Additional information about the primary screen | GR00128-A-2 | 38605 | FBgn0052423 | CG32423 | DRSC34238 | sp | Synthetic lethal with methyl methanesulphonate | | no | | Combinatorial effect with methyl methanesulphonate (2) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Selected genes | dsRNA | rp | rp | | GR00134-A-1 | 38605 | CG32423 | shep | 37863 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00138-A-1 | 38605 | CG32423 | | 37863 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 38605 | CG32423 | shep | 37863 | 0.271604938271604 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 38605 | CG32423 | | 37863 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00159-A | 38605 | | CG32423 | DRSC09771 | np | none | | no | | Muscle assembly and maintenance | RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. | Bai et al. | 2008 | 18359903 | Primary cells | primary embryonic cells | Muscle cell morphology | Fluorescence | np | Selected and random genes | dsRNA | Percentage of muscles with a given phenotype | Severe: > 80 %; medium: ~ 50 % | | GR00190-A-1 | 38605 | FBgn0052423 | shep | 37863 | -0.08 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00214-A-1 | 38605 | FBgn0052423 | shep | DRSC09765 | 1.69 | Increased ERK phosphorylation after EGF stimulation | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 38605 | FBgn0052423 | shep | DRSC09765 | -0.53 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 38605 | FBgn0052423 | shep | DRSC09765 | -0.04 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 38605 | FBgn0052423 | shep | DRSC09765 | 0.26 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 38605 | CG32423 | shep | AMB34628 | -0.31 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 38605 | CG32423 | | 32423R | sp | none | | no | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00273-A-1 | 38605 | FBgn0052423 | shep | DRSC26847 | 3 | Effect on Polycomb (Pc) foci | | | | Polycomb group (PcG) proteins regulation (1) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Pc-GFP protein localization | Fluorescence | DRSC | Genome-wide and transcription factors | dsRNA | Visual inspection | 1 | Additional phenotypic clustering screen available. | GR00282-A | 38605 | CG32423 | shep | 37863 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 38605 | CG32423 | shep | 37863 | 0.1414058596512404 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 38605 | FBgn0052423 | shep | DRSC09771 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00311-A | 38605 | FBgn0052423 | shep | DRSC09765 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00311-A | 38605 | FBgn0052423 | shep | DRSC08748 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | | 38605 | FBgn0052423 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 38605 | CG32423 | | 37863 | np | none | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 38605 | CG32423 | | 37863 | -0.3788394008407572 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 38605 | CG32423 | | BL33996 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 38605 | CG32423 | | GD37863 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |