RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:38720
  • Symbol:Sf3b6
  • Description:Splicing factor 3b subunit 6
DataSource: http://genomernai.dkfz.de/v16/genedetails/38720

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 38720 FBgn0035692 CG13298 DRSC09972 -0.418874904 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 38720 FBgn0035692 CG13298 DRSC09972 -1.351233931 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00021-A-0 38720 FBgn0035692 DRSC09972 strong ERK activation increased no ERK signaling A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman et al. 2006 17086199 Cell line S2R+ ERK signaling Fluorescence DRSC dsRNA Weak, moderate, strong np
GR00031-A-1 38720 HFA09972 -0.3 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 38720 HFA09972 1.2 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00035-A 38720 CG13298 CG13298 KP007 sp none no Alternative splicing Identification of alternative splicing regulators by RNA interference in Drosophila. Park et al. 2004 15492211 Cell line S2 dAdar, para and Dscam mRNA expression Reverse transcription PCR and autoradiography Custom-made RNA-binding protein genes and spliceosomal genes dsRNA np Alternative exon splicing for >
GR00037-A-1 38720 CG13298 np > 2.5 Upregulation of JAK/STAT pathway no JAK/STAT pathway regulation (1) Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. Baeg et al. 2005 16055650 Cell line S2-NP JAK/STAT pathway reporter Luminescence rp Genome-wide dsRNA Fold change of relative luciferase units Complex criteria
38720 FBgn0035692 CG13298 RE19804 sp none no library: DGC2
GR00131-A-1 38720 CG13298 DRSC26462 50 - 75 Altered mitochondrial Ca2+ and/or H+ levels no Mitochondrial Ca2+/H+ antiporter regulation (1) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Jiang et al. 2009 19797662 Cell line S2 mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) Fluorescence np Genome-wide dsRNA Percentage inhibition Class I: > 75; class II: 50-75; class III: 25–50
GR00134-A-1 38720 CG13298 CG13298 31778 np Flightless yes Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 38720 CG13298 CG13298 31777 np Lethal yes late pupal Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-2 38720 CG13298 CG13298 np np Wing posture / locomotion / ability to fly defect or lethal / semi-lethal yes Muscle morphogenesis and function (2) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection Custom-made Selected genes UAS-IR construct rp S19 > 0.5
GR00134-A-4 38720 CG13298 CG13298 31778 np Degenerate myofibrils, fuzzy (broadened) Z-line no Muscle morphogenesis and function (4) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 F-actin protein expression Fluorescence np Selected genes UAS-IR construct np np
GR00135-A-1 38720 CG13298 CG13298 31777 -0.82 Lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 38720 CG13298 31778 0.6 Adult lethal no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00139-A 38720 CG13298 31777 sp Lethal no Fly viability and adult morphology A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Dietzl et al. 2007 17625558 Organism Act5C-GAL4 Adult morphology and viability Visual inspection Custom-made Selected and random genes UAS-IR construct Phenotype strength np Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1 38720 CG13298 CG13298 31777 -0.0416666666666666 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00142-A-1 38720 CG13298 CG13298 31777 0.59375 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00142-A-1 38720 CG13298 CG13298 31778 -0.0571428571428571 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-4 38720 CG13298 31777 sp Completely lethal (pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 38720 CG13298 31778 9 Notum malformation death no partially lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00170-A-1 38720 CG13298 DRSC09972 -4.1 Decreased HIF dependent transcription with desferrioxamine yes HIF-dependent transcription (1) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 1.0 library Genome-wide dsRNA Z-score <
GR00170-A-2 38720 CG13298 DRSC30848 17.7 none no desferrioxamine inhibition < 50 % HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00170-A-2 38720 CG13298 np np none no desferrioxamine inhibition < 50 % HIF-dependent transcription (2) Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. Dekanty et al. 2010 20585616 Cell line S2 Hypoxia inducible HRE reporter Luminescence DRSC 2.0 library Selected genes dsRNA Percentage Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA
GR00183-A 38720 FBgn0035692 CG13298 31777 sp GFP aggregates number, GFP aggregates size, ganglion mother cell shorter lineages, neuroblast underproliferation, lethal no Self-renewal and differentiation in neural stem cells Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Neumueller et al. 2011 21549331 Tissue insc-GAL4 Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability Fluorescence np Genome-wide UAS-IR construct Phenotype strength > 1 Additional information about a secondary screen (KK library)
GR00189-A-1 38720 FBgn0035692 CG13298 BKN22482 np Upregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-3 38720 FBgn0035692 CG13298 np np Upregulation of Notch pathway no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00190-A-1 38720 FBgn0035692 CG13298 31778 0.063 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00190-A-1 38720 FBgn0035692 CG13298 31777 0.099 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00190-A-1 38720 FBgn0035692 CG13298 31777 0.357 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00214-A-1 38720 FBgn0035692 CG13298 DRSC09972 0.38 none 10 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-1 38720 FBgn0035692 CG13298 DRSC09972 1.06 none 0 minutes EGF stimulation yes RTK-Ras-ERK pathway regulation (1) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line S2R+ ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 38720 FBgn0035692 CG13298 DRSC09972 -1.19 none 10 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00214-A-2 38720 FBgn0035692 CG13298 DRSC09972 -0.71 none 30 minutes sSpitz stimulation no RTK-Ras-ERK pathway regulation (2) Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. Friedman et al. 2011 22028469 Cell line Kc167 ERK phosphorylation Fluorescence np Genome-wide dsRNA Z-score > 1.5 OR < -1.5
GR00218-S 38720 CG13298 AMB25440 0.47 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00282-A 38720 CG13298 CG13298 105704 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 38720 CG13298 CG13298 31777 np none rudimentary ovaries piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 38720 CG13298 CG13298 105704 -0.6479375305322173 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00308-A 38720 CG13298 CG13298 np 2 Decreased cell aggregation Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 38720 FBgn0035692 CG13298 DRSC09972 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00314-A 38720 FBgn0035692 CG13298 RNA006085 62.92 Decreased MAPK phosphorylation hit in validation screens RAS-related MAPK activation A Functional Screen Reveals an Extensive Layer of Transcriptional and Splicing Control Underlying RAS/MAPK Signaling in Drosophila Ashton-Beaucage et al. 2014 24643257 Cell line S2 MAPK phosphorylation Fluorescence Custom-made Genome-wide dsRNA pMAPK signal (%GFP dsRNA) np S2 cells stably expressed pMet-RasV12. See comment for hits in validation screens. Data deposited at http://www.bioinfo.iric.ca/iricrnai
GR00315-A-1 38720 CG13298 CG13298 105704 np Effect on follicular epithelium morphology further phenotype data in classification screen Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00315-A-2 38720 CG13298 CG13298 105704 np Defect in formation and maturation phase Follicular epithelium development (2) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Selected genes UAS-IR construct Visual inspection np For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00335-A 38720 FBgn0035692 CG13298 HM04033 np Agametic Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 38720 FBgn0035692 CG13298 GL00579 np Agametic Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
38720 FBgn0035692 np np sp none
38720 np np HFA09972 -0.45 none fold change: 1.2
GR00379-A-1 38720 CG13298 105704 0.6207218360957243 none Reduced eggs/larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00379-A-2 38720 CG13298 105704 np No germline, 100% Defects in female germline Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 Vasa AND 1B1 expression Immunofluorescence VDRC Selected genes UAS-IR construct np np Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A 38720 105704 0.41 none Regulation of olfactory memory formation Identification of genes that promote or inhibit olfactory memory formation in Drosophila. Walkinshaw et al. 2015 25644700 Organism Nsyb-GAL4 T-maze performance Visual inspection VDRC Selected genes UAS-IR construct Performance index, sp High performer in olfactory memory formation: > Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A 38720 CG13298 GD31778 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.