| GR00002-A | 39329 | FBgn0015278 | Pi3K68D | DRSC11253 | -0.471183918 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 39329 | FBgn0015278 | Pi3K68D | DRSC11253 | 0.370195398 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00031-A-1 | 39329 | | | HFA11253 | -0.6 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 39329 | | | HFA11253 | -0.2 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 39329 | FBgn0015278 | Pi3K68D | np | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 39329 | FBgn0015278 | Pi3K68D | LD15217 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 39329 | FBgn0015278 | Pi3K68D | LD28067 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 39329 | FBgn0015278 | Pi3K68D | CT18836 | sp | none | | no | library: PHOSPHO | | | | | | | | | | | | | | | | | 39329 | FBgn0015278 | Pi3K68D | CT41611 | sp | none | | no | library: PHOSPHO | | | | | | | | | | | | | | | | GR00051-A-1 | 39329 | CG11621 | Pi3K68D | np | np | Decreased Candida albicans phagocytosis | | yes | validated (34 % cells phagocytosing) | Candida albicans phagocytosis (1) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | C. albicans phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing C. albicans | < | | GR00051-A-2 | 39329 | CG11621 | Pi3K68D | np | 20 | Decreased Escherichia coli phagocytosis | | yes | | Candida albicans phagocytosis (2) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | Escherichia coli phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing E. coli | < | | GR00051-A-3 | 39329 | CG11621 | Pi3K68D | np | 44 | Decreased latex beads phagocytosis | | yes | | Candida albicans phagocytosis (3) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | Latex beads phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing latex beads | < | | GR00070-A-0 | 39329 | FBgn0015278 | Pi3K68D | np | np | none | | no | | Endocytosis | Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation. | Korolchuk | 2007 | 18057029 | Cell line | S2 | mBSA uptake | High content (microscopy) | Custom-made library | | dsRNA | Visual inspection | np | | GR00083-A | 39329 | CG11621 | Pi3k68D | np | 43.09 | Decreased Brucella melitensis infection | | yes | | Brucella melitensis infection | RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication. | Qin et al. | 2008 | 18654626 | Cell line | S2 | Intracellular bacterial GFP protein expression | Fluorescence | Drosophila RNAi Library Release 1.0 | Selected genes | dsRNA | Relative infection | > 2 standard deviations below untreated control | Additional information about secondary screens (gentamicin protection assays) | GR00128-A-2 | 39329 | FBgn0015278 | CG11621 | DRSC32163 | sp | Synthetic lethal with methyl methanesulphonate | | no | | Combinatorial effect with methyl methanesulphonate (2) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Selected genes | dsRNA | rp | rp | | GR00134-A-1 | 39329 | CG11621 | Pi3K68D | 16239 | np | Flightless | | yes | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 39329 | CG11621 | Pi3K68D | 16240 | np | none | | yes | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-4 | 39329 | CG11621 | Pi3K68D | 16239 | np | Missing indirect flight muscles, degenerate myofibrils, no sarcomere | | no | | Muscle morphogenesis and function (4) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | F-actin protein expression | Fluorescence | np | Selected genes | UAS-IR construct | np | np | | GR00135-A-1 | 39329 | CG11621 | Pi3K68D | 16240 | 0.23 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 39329 | CG11621 | | 16240 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 39329 | CG11621 | Pi3K68D | 16240 | -0.793650793650793 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 39329 | CG11621 | | 16239 | 0 | none | | no | partially lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 39329 | CG11621 | | 16240 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00159-A | 39329 | | Pi3K68D | DRSC11253 | np | none | | no | | Muscle assembly and maintenance | RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. | Bai et al. | 2008 | 18359903 | Primary cells | primary embryonic cells | Muscle cell morphology | Fluorescence | np | Selected and random genes | dsRNA | Percentage of muscles with a given phenotype | Severe: > 80 %; medium: ~ 50 % | | GR00189-A-1 | 39329 | FBgn0015278 | Pi3K68D | BKN27399 | np | Upregulation of Notch pathway | | yes | | Notch pathway regulation (1) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Cell line | S2 | Notch pathway reporter | Luminescence | BKN | Genome-wide | dsRNA | Complex, sp | Complex criteria | | GR00189-A-2 | 39329 | FBgn0015278 | Pi3K68D | 11621R-1 | np | none | | yes | C96-GAL4 | Notch pathway regulation (2) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | en-GAL4, C96-GAL4 and vg-GAL4 | Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability | Fluorescence and visual inspection | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00189-A-2 | 39329 | FBgn0015278 | Pi3K68D | 16240 | np | none | | yes | en-GAL4 | Notch pathway regulation (2) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | en-GAL4, C96-GAL4 and vg-GAL4 | Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability | Fluorescence and visual inspection | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00189-A-2 | 39329 | FBgn0015278 | Pi3K68D | 11621R-1 | np | none | | yes | en-GAL4 | Notch pathway regulation (2) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | en-GAL4, C96-GAL4 and vg-GAL4 | Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability | Fluorescence and visual inspection | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00189-A-2 | 39329 | FBgn0015278 | Pi3K68D | 11621R-2 | np | none | | yes | en-GAL4 | Notch pathway regulation (2) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | en-GAL4, C96-GAL4 and vg-GAL4 | Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability | Fluorescence and visual inspection | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00189-A-3 | 39329 | FBgn0015278 | Pi3K68D | np | np | Upregulation of Notch pathway | | no | | Notch pathway regulation (3) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | GMR-GAL4 | Notch pathway reporter | Fluorescence | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Average fluorescent intensity | Complex criteria | | GR00190-A-1 | 39329 | FBgn0015278 | Pi3K68D | 16240 | -0.453 | Decreased triglyceride expression | | yes | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00190-A-2 | 39329 | FBgn0015278 | Pi3K68D | 16240 | 0.181 | none | | no | | Adiposity regulation (2) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Tissue | nsyb-GAL4 | Total fly triglyceride expression | Colorimetrics | np | Selected genes | UAS-IR construct | Triglyceride change | > 0.25 OR <-0.25 (change >25%) | Additional information about the primary screen | GR00190-A-3 | 39329 | FBgn0015278 | Pi3K68D | 16240 | 0.052 | none | | no | | Adiposity regulation (3) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Tissue | C57-GAL4 | Total fly triglyceride expression | Colorimetrics | np | Selected genes | UAS-IR construct | Triglyceride change | > 0.25 OR <-0.25 (change >25%) | Additional information about the primary screen | GR00190-A-4 | 39329 | FBgn0015278 | Pi3K68D | 16240 | -0.153 | none | | no | | Adiposity regulation (4) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Tissue | oe-GAL4 | Total fly triglyceride expression | Colorimetrics | np | Selected genes | UAS-IR construct | Triglyceride change | > 0.25 OR <-0.25 (change >25%) | Additional information about the primary screen | GR00190-A-5 | 39329 | FBgn0015278 | Pi3K68D | 16240 | 0.259 | Increased triglyceride expression | | no | | Adiposity regulation (5) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Tissue | ppl-GAL4 | Total fly triglyceride expression | Colorimetrics | np | Selected genes | UAS-IR construct | Triglyceride change | > 0.25 OR <-0.25 (change >25%) | Additional information about the primary screen | GR00191-A-1 | 39329 | CG11621 | Pi3K68D | np | np | none | | yes | | Blood cell homeostasis regulation (1) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-2 | 39329 | CG11621 | Pi3K68D | np | np | none | | no | | Blood cell homeostasis regulation (2) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00201-A-1 | 39329 | FBgn0015278 | Pi3K68D | CG11621 (UCSF), Pi3K68D (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (1) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | S2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-2 | 39329 | FBgn0015278 | Pi3K68D | CG11621 (UCSF), Pi3K68D (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (2) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | S2R+ | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-3 | 39329 | FBgn0015278 | Pi3K68D | CG11621 (UCSF), Pi3K68D (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (3) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | Kc167 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-4 | 39329 | FBgn0015278 | Pi3K68D | CG11621 (UCSF), Pi3K68D (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (4) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG2-c2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-5 | 39329 | FBgn0015278 | Pi3K68D | CG11621 (UCSF), Pi3K68D (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (5) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG3-c1 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-6 | 39329 | FBgn0015278 | Pi3K68D | CG11621 (UCSF), Pi3K68D (Baum_Lab) | np | none | | no | | Actin organization and cell morphology (6) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG3-c2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00202-A | 39329 | FBgn0015278 | Pi3K68D | np | sp | Decreased number of vaccinia virus infected cells | | yes | validated: non-overlapping dsRNAs used | Vaccinia virus infection | A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. | Moser et al. | 2010 | 20585561 | Cell line | DL1 | Virally encoded beta-galactosidase protein expression | Fluorescence | Custom-made | Kinases, phosphatases and selected genes | dsRNA | Z-score | < -2 in both duplicates | Author-reviewed data | | 39329 | FBgn0015278 | Pi3K68D | np | < -2 | Decreased BiP signal peptide and firefly luciferase fusion protein expression | | yes | | | | | | | | | | | | | | | | | GR00218-S | 39329 | CG11621 | Pi3K68D | AMB34333 | -0.43 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00238-S-1 | 39329 | CG11621 | Pi3K68D | 11621R-1 | sp | none | | no | library: NIG | Muscle development and maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | Mef2-GAL4 | Climbing ability and viability | Negative geotaxis assay and visual inspection | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00239-A-1 | 39329 | CG11621 | | 11621R | sp | Increased GlcNAcb1-4GlcNAc Chp glycosylation | | yes | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00239-A-2 | 39329 | CG11621 | | 11621R | np | Effect on Chp glycosylation | | no | rank 2 | Glycosylation regulation (2) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation | Luminescence | np | Selected genes | UAS-IR construct | p-value | < 0.005 | | GR00245-A | 39329 | FBgn0015278 | PI3K68D | DRSC11253 | np | Increased formation of mutant Huntingtin (Httex1-Qn) protein aggregates | | yes | validated | Huntingtin (Htt) aggregates formation | A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila. | Zhang et al. | 2010 | 20100940 | Cell line | S2 | Mutant Httex1-Qn-eGFP protein expression and DNA content | Fluorescence | Version 1 and np | Genome-wide | dsRNA | Aggregate number, size, and signal intensity | > 2 standard deviations | Additional information about secondary screens (reproducibility and non-overlaping dsRNAs) | GR00282-A | 39329 | CG11621 | Pi3K68D | 16240 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 39329 | CG11621 | Pi3K68D | 16239 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 39329 | CG11621 | Pi3K68D | 16240 | 0.9188177110855684 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 39329 | FBgn0015278 | Pi3K68D | DRSC11253 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 39329 | CG11621 | Pi3K68D | 109582 | np | none | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00335-A | 39329 | FBgn0015278 | Pi3K68D | GL00159 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 39329 | FBgn0015278 | Pi3K68D | JF01193 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 39329 | FBgn0015278 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 39329 | CG11621 | | 116212 | np | none | | | NIG | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00370-A | 39329 | FBgn0015278 | Pi3K68D | | -0.86 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00370-A | 39329 | FBgn0015278 | Pi3K68D | | 0.32 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00370-A | 39329 | FBgn0015278 | Pi3K68D | | -0.22 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00379-A-1 | 39329 | CG11621 | | 16240 | -0.2538706348877246 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00385-A | 39329 | | | 109582 | 0.30201365964439647 | none | | | | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00388-A | 39329 | CG11621 | | BL31252 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 39329 | CG11621 | | GD16240 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 39329 | CG11621 | | GD16239 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |