RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:39610
  • Symbol:mop
  • Description:myopic
DataSource: http://genomernai.dkfz.de/v16/genedetails/39610

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 39610 FBgn0036448 CG9311 DRSC11065 -2.133152429 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 39610 FBgn0036448 CG9311 DRSC11065 -0.235387894 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 39610 FBgn0036448 CG9311 DRSC11065 -0.212788558 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00031-A-1 39610 HFA11065 1.3 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 39610 HFA11065 0.5 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00038-A-0 39610 FBgn0036448 mop np **** Escape pff LLO- strain no L. monocytogenes infection Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. Cheng et al. 2005 16157870 Cell line S2 L. monocytogenes infection High content (microscopy) Custom-made dsRNA Visual inspection np
39610 FBgn0036448 CG9311 SD03094 sp none no library: DGC1
39610 FBgn0036448 CG9311 CT26513 sp none no library: PHOSPHO
GR00134-A-1 39610 CG9311 CG9311 14174 np Lethal yes adult/pharate Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-2 39610 CG9311 CG9311 np np Wing posture / locomotion / ability to fly defect or lethal / semi-lethal no Muscle morphogenesis and function (2) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection Custom-made Selected genes UAS-IR construct rp S19 > 0.5
GR00135-A-1 39610 CG9311 CG9311 14173 -1.17 Developmentally lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 39610 CG9311 CG9311 14174 7.86 Increased noxious heat avoidance yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 39610 CG9311 14173 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 39610 CG9311 CG9311 14173 -2.07792207792207 Decreased viability after Serratia marcescens infection yes Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00142-A-2 39610 CG9311 CG9311 np sp none yes Serratia marcescens infection (2) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Tissue HML-GAL4 Viability Fly count np Selected genes UAS-IR construct Complex, sp Significantly reduced survival (p < 0.05)
GR00142-A-4 39610 CG9311 CG9311 np sp Decreased viability after Serratia marcescens infection no Serratia marcescens infection (4) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Tissue NP1-GAL4 Viability Fly count np Selected genes UAS-IR construct Complex, sp Significantly reduced survival (p < 0.05)
GR00144-A-4 39610 CG9311 14173 sp Completely lethal (pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 39610 CG9311 14174 sp Completely lethal (pupal) no Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00159-A 39610 CG9311 DRSC11065 np none no Muscle assembly and maintenance RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Bai et al. 2008 18359903 Primary cells primary embryonic cells Muscle cell morphology Fluorescence np Selected and random genes dsRNA Percentage of muscles with a given phenotype Severe: > 80 %; medium: ~ 50 %
GR00189-A-1 39610 FBgn0036448 CG9311 BKN20331 np Upregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 39610 FBgn0036448 mop 14174 sp Downregulation of Notch pathway; adult wing margin: notches yes C96-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 39610 FBgn0036448 mop 9311R-3 sp Downregulation of Notch pathway; wing disc NRE:EGFP: down; lethal yes en-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 39610 FBgn0036448 mop 14174 sp Downregulation of Notch pathway; wing disc NRE:EGFP: down; wing disc myrRFP: accumulation; lethal yes en-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 39610 FBgn0036448 mop 9311R-3 sp Upregulation of Notch pathway; wing disc NRE:EGFP: up; lethal yes en-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-2 39610 FBgn0036448 mop 9311R-3 sp Wing disc NRE:EGFP: up, broader; lethal yes en-GAL4; final gene scoring: up- and downregulation of Notch pathway Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 39610 FBgn0036448 mop np np Upregulation of Notch pathway no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00191-A-1 39610 CG9311 mop np np Increased melanotic mass formation yes Blood cell homeostasis regulation (1) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-2 39610 CG9311 mop np np none yes Blood cell homeostasis regulation (2) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct np np
GR00191-A-3 39610 CG9311 mop np 1.7 none yes Blood cell homeostasis regulation (3) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue srp-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-4 39610 CG9311 mop np 0 none yes Blood cell homeostasis regulation (4) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue hml∆-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00191-A-5 39610 CG9311 mop np 2 none no Blood cell homeostasis regulation (5) An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. Avet-Rochex et al. 2010 20540764 Tissue cg-GAL4 Melanotic mass formation Microscopy np Selected genes UAS-IR construct Tumor index >
GR00202-A 39610 FBgn0036448 CG9311 np sp Decreased number of vaccinia virus infected cells yes validated: non-overlapping dsRNAs used Vaccinia virus infection A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. Moser et al. 2010 20585561 Cell line DL1 Virally encoded beta-galactosidase protein expression Fluorescence Custom-made Kinases, phosphatases and selected genes dsRNA Z-score < -2 in both duplicates Author-reviewed data
39610 FBgn0036448 CG9311 np < -2 Decreased BiP signal peptide and firefly luciferase fusion protein expression yes
GR00218-S 39610 CG9311 AMB22920 0.34 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 39610 CG9311 9311R np Decreased Chp protein expression no not detectable Chp level Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 39610 CG9311 mop 14174 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 39610 CG9311 mop 14173 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 39610 CG9311 mop 104860 0.2744687097907574 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00308-A 39610 CG9311 CG9311 np 1.67 Decreased cell aggregation validated in secondary screen Cadherin-mediated cell-cell adhesion A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion Toret et al. 2014 24446484 Cell line S2 Cell aggregation Fluorescence V2 RNAi library (Thermo Fisher Scientific) Genome-wide dsRNA Visual inspection; average of 3 replicates; 0 > 1.5 Additional secondary screen available. S2 cells stably express DE-cadherin.
GR00311-A 39610 FBgn0036448 mop DRSC11065 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 39610 CG9311 mop 104860 np Effect on follicular epithelium morphology further phenotype data in classification screen Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00315-A-2 39610 CG9311 mop 104860 np Defect in formation phase Follicular epithelium development (2) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Selected genes UAS-IR construct Visual inspection np For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available.
GR00326-A 39610 FBgn0036448 mop 104860 np none Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
GR00335-A 39610 FBgn0036448 mop HM05008 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 39610 FBgn0036448 mop HMS00706 np Embryonic lethal many embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
39610 FBgn0036448 np np sp none
GR00367-S 39610 CG9311 14174 np lethal or adult morphology phenotype VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 39610 CG9311 104860 -0.36534711116668317 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00388-A 39610 CG9311 BL28522 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 39610 CG9311 BL32916 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 39610 CG9311 KK104860 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 39610 CG9311 GD14174 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 39610 CG9311 BL34085 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.