| GR00002-A | 39744 | FBgn0000212 | brm | DRSC11330 | 6.853479214 | Increased lipid droplet area | | yes | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 39744 | FBgn0000212 | brm | DRSC11330 | 8.695161858 | Increased lipid droplet area | | yes | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00003-A-0 | 39744 | FBgn0000212 | | DRSC11330 | 3 (0.0027) | Inhibition of dengue virus propagation | | no | | Insect dengue virus infection | Discovery of insect and human dengue virus host factors. | Sessions et al. | 2009 | 19396146 | Cell line | D.Mel-2 (S2) | Dengue virus propagation | High content (microscopy) | DRSC | | dsRNA | Fold inhibition (p-value) | 1.5 | | GR00004-A-0 | 39744 | | brm | DRSC11330 | 0.55 | siRNA reporter downregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00004-A-0 | 39744 | | brm | DRSC11330 | 0.69 | miRNA reporter downregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00004-A-0 | 39744 | | brm | DRSC11330 | 0.43 | Endo-siRNA reporter downregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00021-A-0 | 39744 | FBgn0000212 | | DRSC11330 | strong | ERK activation reduced | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 39744 | | | HFA11330 | 2.2 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 39744 | | | HFA11330 | 0.7 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00037-A-1 | 39744 | | brm | np | > 1.5 | Upregulation of JAK/STAT pathway | | no | | JAK/STAT pathway regulation (1) | Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. | Baeg et al. | 2005 | 16055650 | Cell line | S2-NP | JAK/STAT pathway reporter | Luminescence | rp | Genome-wide | dsRNA | Fold change of relative luciferase units | Complex criteria | | | 39744 | FBgn0000212 | brm | np | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 39744 | FBgn0000212 | brm | LD36356 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | GR00051-A-1 | 39744 | CG5942 | brm | np | np | Decreased Candida albicans phagocytosis | | yes | validated (26 % cells phagocytosing) | Candida albicans phagocytosis (1) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | C. albicans phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing C. albicans | < | | GR00051-A-2 | 39744 | CG5942 | brm | np | 42 | Decreased Escherichia coli phagocytosis | | yes | | Candida albicans phagocytosis (2) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | Escherichia coli phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing E. coli | < | | GR00051-A-3 | 39744 | CG5942 | brm | np | 33 | Decreased latex beads phagocytosis | | yes | | Candida albicans phagocytosis (3) | Identification of Drosophila gene products required for phagocytosis of Candida albicans. | Stroschein-Stevenson et al. | 2006 | 16336044 | Cell line | S2 | Latex beads phagocytosis, actin protein expression and DNA content | Fluorescence | rp | Selected genes | dsRNA | Percentage cells phagocytosing latex beads | < | | GR00052-A | 39744 | | brm | DRSC11330 | np | Decreased Ca2+ uptake after thapsigargin stimulation | | no | | Store-operated calcium influx regulation | Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity. | Zhang et al. | 2006 | 16751269 | Cell line | S2 | Ca2+ uptake | Fluorescence | np | Genome-wide | dsRNA | Z-score | < -3 | | GR00062-A | 39744 | FBgn0000212 | brm | np | 4 | Decreased Ca2+ influx | | | final hit | Ca2+ channel regulation | CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. | Vig et al. | 2006 | 16645049 | Cell line | S2R+ | Ca2+ influx | Fluorescence | np | Genome-wide | dsRNA | Complex, sp | > | | GR00065-A | 39744 | CG5942 | brm | brm | np | Misrouting | | yes | | Dendrite pattern formation | Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. | Parrish et al. | 2006 | 16547170 | Tissue | GAL4221 | mCD8 protein expression | Fluorescence | Custom-made | Transcription factors | UAS-IR construct | np | Phenotypes in multiple blind tests | Additional information about secondary screens | | 39744 | FBgn0000212 | brm | np | 0.638 | Decreased mitochondrial citrate synthase activity | | yes | | | | | | | | | | | | | | | | | GR00130-A | 39744 | FBgn0000212 | brm | np | >= 2 | Increased mutant human huntingtin aggregation | | no | | Mutant human huntingtin aggregation | RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. | Doumanis et al. | 2009 | 19789644 | Cell line | BG2-c2 | Nhtt(62Q)EGFP aggregate number and size | Fluorescence | OpenBiosystems RNAi library | Selected genes | dsRNA | Z-score | Suppressor: < | | GR00131-A-1 | 39744 | | brm | DRSC11330 | 50 - 75 | Altered mitochondrial Ca2+ and/or H+ levels | | yes | | Mitochondrial Ca2+/H+ antiporter regulation (1) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Genome-wide | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00131-A-1 | 39744 | | brm | DRSC26226 | 50 - 75 | Altered mitochondrial Ca2+ and/or H+ levels | | yes | | Mitochondrial Ca2+/H+ antiporter regulation (1) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Genome-wide | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00131-A-2 | 39744 | | brm | DRSC11330, DRSC30901 | 50 - 75 | Altered mitochondrial Ca2+ and/or H+ levels | | no | | Mitochondrial Ca2+/H+ antiporter regulation (2) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Selected genes | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00134-A-1 | 39744 | CG5942 | brm | 37720 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 39744 | CG5942 | brm | 37721 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 39744 | CG5942 | brm | 37720 | 0.02 | Developmentally lethal | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 39744 | CG5942 | brm | 37721 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 39744 | CG5942 | | 37721 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 39744 | CG5942 | brm | 37721 | 0.723076923076923 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 39744 | CG5942 | | 37720 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 39744 | CG5942 | | 37721 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00170-A-1 | 39744 | | brahma | DRSC11330 | -8.5 | Decreased HIF dependent transcription with desferrioxamine | | yes | | HIF-dependent transcription (1) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 1.0 library | Genome-wide | dsRNA | Z-score | < | | GR00170-A-2 | 39744 | | brahma | DRSC30902 | 89 | Decreased HIF dependent transcription with desferrioxamine | | yes | group A | HIF-dependent transcription (2) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 2.0 library | Selected genes | dsRNA | Percentage | Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA | | GR00170-A-2 | 39744 | | brahma | DRSC30901 | 85 | Decreased HIF dependent transcription with desferrioxamine | | yes | group A | HIF-dependent transcription (2) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 2.0 library | Selected genes | dsRNA | Percentage | Group A: > 75 %; group B: 50 – 75 % by at least one dsRNA | | GR00170-A-3 | 39744 | | Brahma | DRSC30902 | 83.9 | Decreased HIF-dependent transcription under hypoxia | | no | | HIF-dependent transcription (3) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 2.0 library | Selected genes | dsRNA | Percentage | > | | GR00170-A-3 | 39744 | | Brahma | DRSC30901 | 84.8 | Decreased HIF-dependent transcription under hypoxia | | no | | HIF-dependent transcription (3) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 2.0 library | Selected genes | dsRNA | Percentage | > | | GR00176-A | 39744 | brm | CG5942 | HFA11330 | 4.87 | Upregulation of Toll pathway after hEGF stimulation | | no | | Toll-dependent innate immune pathway regulation | A large-scale RNAi screen identifies Deaf1 as a regulator of innate immune responses in Drosophila. | Kuttenkeuler et al. | 2010 | 20375635 | Cell line | SL2 | Toll pathway reporter | Luminescence | Custom-made | Transcription factors | dsRNA | Z-score | < -2.5 OR > 2.5 | | GR00183-A | 39744 | FBgn0000212 | brm | 37721 | sp | Neuroblast more large cells, ganglion mother cell shorter lineages, neuroblast overproliferation, neuroblast underproliferation, lethal | | yes | | Self-renewal and differentiation in neural stem cells | Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. | Neumueller et al. | 2011 | 21549331 | Tissue | insc-GAL4 | Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability | Fluorescence | np | Genome-wide | UAS-IR construct | Phenotype strength | > 1 | Additional information about a secondary screen (KK library) | GR00183-A | 39744 | FBgn0000212 | brm | 37720 | sp | GFP aggregates size, neuroblast more large cells, ganglion mother cell shorter lineages, neuroblast overproliferation, neuroblast underproliferation, lethal | | yes | | Self-renewal and differentiation in neural stem cells | Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. | Neumueller et al. | 2011 | 21549331 | Tissue | insc-GAL4 | Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability | Fluorescence | np | Genome-wide | UAS-IR construct | Phenotype strength | > 1 | Additional information about a secondary screen (KK library) | GR00185-A | 39744 | CG5942 | Brm | np | 91 | none | | no | 20 min heat shock | Pol II elongation | Spt6 enhances the elongation rate of RNA polymerase II in vivo. | Ardehali et al. | 2009 | 19279664 | Cell line | S2 | Hsp70 mRNA expression | Real-time qPCR | Custom-made | Transcription factors | dsRNA | Percentage | < | | GR00190-A-1 | 39744 | FBgn0000212 | brm | 37721 | -0.022 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00214-A-1 | 39744 | FBgn0000212 | brm | DRSC11330 | 0.36 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 39744 | FBgn0000212 | brm | DRSC11330 | -2.09 | Decreased ERK phosphorylation after EGF stimulation | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 39744 | FBgn0000212 | brm | DRSC11330 | 2.42 | Increased ERK phosphorylation after sSpitz and EGF stimulation | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 39744 | FBgn0000212 | brm | DRSC11330 | 0.61 | none | 10 minutes sSpitz stimulation | yes | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 39744 | CG5942 | brm | AMB31683 | 0.02 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-4 | 39744 | CG5942 | | 37721 | sp | none | | no | | Glycosylation regulation (4) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00245-A | 39744 | FBgn0000212 | brm | DRSC11330 | np | Decreased formation of mutant Huntingtin (Httex1-Qn) protein aggregates | | yes | validated | Huntingtin (Htt) aggregates formation | A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila. | Zhang et al. | 2010 | 20100940 | Cell line | S2 | Mutant Httex1-Qn-eGFP protein expression and DNA content | Fluorescence | Version 1 and np | Genome-wide | dsRNA | Aggregate number, size, and signal intensity | > 2 standard deviations | Additional information about secondary screens (reproducibility and non-overlaping dsRNAs) | GR00273-A-1 | 39744 | FBgn0000212 | brm | DRSC26226 | 3 | Effect on Polycomb (Pc) foci | | | | Polycomb group (PcG) proteins regulation (1) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Pc-GFP protein localization | Fluorescence | DRSC | Genome-wide and transcription factors | dsRNA | Visual inspection | 1 | Additional phenotypic clustering screen available. | GR00273-A-1 | 39744 | FBgn0000212 | brm | DRSC11330 | 3 | Effect on Polycomb (Pc) foci | | | | Polycomb group (PcG) proteins regulation (1) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Pc-GFP protein localization | Fluorescence | DRSC | Genome-wide and transcription factors | dsRNA | Visual inspection | 1 | Additional phenotypic clustering screen available. | GR00273-A-1 | 39744 | FBgn0000212 | brm | DRSC11330 | 3 | Effect on Polycomb (Pc) foci | | | | Polycomb group (PcG) proteins regulation (1) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Pc-GFP protein localization | Fluorescence | DRSC | Genome-wide and transcription factors | dsRNA | Visual inspection | 1 | Additional phenotypic clustering screen available. | GR00273-A-2 | 39744 | FBgn0000212 | brm | DRSC11330 | np | Increased Polycomb (Pc) foci intensity | | | | Polycomb group (PcG) proteins regulation (2) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Polycomb-GFP protein localization | Fluorescence | DRSC | Selected genes | dsRNA | Visual inspection | np | | GR00282-A | 39744 | CG5942 | brm | 37720 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 39744 | CG5942 | brm | 37721 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 39744 | CG5942 | brm | 37721 | 0.27307042398507725 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 39744 | FBgn0000212 | brm | DRSC11330 | np | Actin defect, microtubule defect, other cellular phenotype | | | cell shape processes or spiky or stretchy, increased number of actin stress fibres, increased peripheral actin, microtubule processes | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00335-A | 39744 | FBgn0000212 | brm | HMS00050 | np | F1 lethal | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 39744 | FBgn0000212 | brm | HM04019 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 39744 | FBgn0000212 | brm | GL00090 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 39744 | FBgn0000212 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 39744 | CG5942 | | 37720 | np | lethal or adult morphology phenotype | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 39744 | CG5942 | | 37721 | -0.31432311615991704 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 39744 | CG5942 | | GD37721 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 39744 | CG5942 | | BL31712 | | none | | | excess of ISC or EB | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 39744 | CG5942 | | BL34520 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |