| GR00002-A | 40170 | FBgn0013591 | Mi-2 | DRSC09640 | 0.451614716 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40170 | FBgn0013591 | Mi-2 | DRSC09640 | 0.150110981 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40170 | FBgn0013591 | Mi-2 | DRSC11222 | -0.121767848 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40170 | FBgn0013591 | Mi-2 | DRSC11222 | 2.7328563 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00021-A-0 | 40170 | FBgn0013591 | | DRSC11222 | strong | ERK activation reduced | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 40170 | | | HFA11222 | -1.7 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 40170 | | | HFA11222 | 1.5 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00035-A | 40170 | CG8103 | Mi-2 | KP075 | sp | none | | no | | Alternative splicing | Identification of alternative splicing regulators by RNA interference in Drosophila. | Park et al. | 2004 | 15492211 | Cell line | S2 | dAdar, para and Dscam mRNA expression | Reverse transcription PCR and autoradiography | Custom-made | RNA-binding protein genes and spliceosomal genes | dsRNA | np | Alternative exon splicing for > | | GR00036-A | 40170 | CG8103 | Mi-2 | DRSC11222 | strong | Decreased GFP protein expression | | yes | decreased intracellular infection | Intracellular Listeria monocytogenes infection | Genome-wide RNAi screen for host factors required for intracellular bacterial infection. | Agaisse et al. | 2005 | 16020693 | Cell line | SL2 | GFP protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | | GR00040-A-0 | 40170 | FBgn0013591 | | DRSC11222 | 5.9 | Increased Wnt reporter activity | | no | | Wnt signaling | Functional genomic analysis of the Wnt-wingless signaling pathway. | DasGupta et al. | 2005 | 15817814 | Cell line | Clone 8 | Wnt signaling | Dual luciferase | DRSC | | dsRNA | Z-score | > | | | 40170 | FBgn0013591 | Mi-2 | LD32039 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | GR00065-A | 40170 | CG8103 | Mi-2 | Mi-2 | np | Decreased arborization | | yes | | Dendrite pattern formation | Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. | Parrish et al. | 2006 | 16547170 | Tissue | GAL4221 | mCD8 protein expression | Fluorescence | Custom-made | Transcription factors | UAS-IR construct | np | Phenotypes in multiple blind tests | Additional information about secondary screens | GR00082-A | 40170 | | Mi-2 | HFA11222 | np | Increased SUMO-dependent transcriptional repression | | yes | | SUMO-dependent transcriptional repression | Identification of SUMO-dependent chromatin-associated transcriptional repression components by a genome-wide RNAi screen. | Stielow et al. | 2008 | 18374648 | Cell line | Kc167 | SUMOylation pathway reporter | Luminescence | rp | Genome-wide | dsRNA | FLuc/Rluc ratio | Complex criteria | Additional information about secondary screens | GR00131-A-1 | 40170 | | Mi-2 | DRSC11222 | 25 - 50 | Altered mitochondrial Ca2+ and/or H+ levels | | no | | Mitochondrial Ca2+/H+ antiporter regulation (1) | Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. | Jiang et al. | 2009 | 19797662 | Cell line | S2 | mt-pericam protein expression (reporter of mitochondrial Ca2+ and H+ levels) | Fluorescence | np | Genome-wide | dsRNA | Percentage inhibition | Class I: > 75; class II: 50-75; class III: 25–50 | | GR00134-A-1 | 40170 | CG8103 | Mi-2 | 10766 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 40170 | CG8103 | Mi-2 | 10766 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 40170 | CG8103 | | 10766 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 40170 | CG8103 | Mi-2 | 10766 | 0.309859154929577 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 40170 | CG8103 | | 10766 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00176-A | 40170 | Mi-2 | CG8103 | HFA11222 | -5.33 | Downregulation of Toll pathway after hEGF stimulation | | no | | Toll-dependent innate immune pathway regulation | A large-scale RNAi screen identifies Deaf1 as a regulator of innate immune responses in Drosophila. | Kuttenkeuler et al. | 2010 | 20375635 | Cell line | SL2 | Toll pathway reporter | Luminescence | Custom-made | Transcription factors | dsRNA | Z-score | < -2.5 OR > 2.5 | | GR00185-A | 40170 | CG8103 | Mi2 | np | 163 | none | | no | 20 min heat shock | Pol II elongation | Spt6 enhances the elongation rate of RNA polymerase II in vivo. | Ardehali et al. | 2009 | 19279664 | Cell line | S2 | Hsp70 mRNA expression | Real-time qPCR | Custom-made | Transcription factors | dsRNA | Percentage | < | | GR00189-A-1 | 40170 | FBgn0013591 | Mi-2 | BKN20613 | np | Upregulation of Notch pathway | | yes | | Notch pathway regulation (1) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Cell line | S2 | Notch pathway reporter | Luminescence | BKN | Genome-wide | dsRNA | Complex, sp | Complex criteria | | GR00189-A-2 | 40170 | FBgn0013591 | Mi-2 | 10766 | np | none | | yes | en-GAL4 | Notch pathway regulation (2) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | en-GAL4, C96-GAL4 and vg-GAL4 | Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability | Fluorescence and visual inspection | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00189-A-3 | 40170 | FBgn0013591 | Mi-2 | np | np | none | | no | | Notch pathway regulation (3) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | GMR-GAL4 | Notch pathway reporter | Fluorescence | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Average fluorescent intensity | Complex criteria | | GR00190-A-1 | 40170 | FBgn0013591 | Mi-2 | 10766 | -0.407 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00214-A-1 | 40170 | FBgn0262519 | Mi-2 | DRSC11222 | -1.86 | Decreased ERK phosphorylation after EGF stimulation | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 40170 | FBgn0262519 | Mi-2 | DRSC11222 | -0.13 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 40170 | FBgn0262519 | Mi-2 | DRSC11222 | 4.6 | Increased ERK phosphorylation after sSpitz and EGF stimulation | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 40170 | FBgn0262519 | Mi-2 | DRSC11222 | 2.17 | Increased ERK phosphorylation after sSpitz and EGF stimulation | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 40170 | CG8103 | Mi-2 | AMB21600 | 0.7 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00282-A | 40170 | CG8103 | Mi-2 | 10766 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 40170 | CG8103 | Mi-2 | 107204 | np | none | | | rudimentary ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 40170 | CG8103 | Mi-2 | 107204 | -0.8237356401268332 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 40170 | FBgn0013591 | Mi-2 | DRSC11222 | np | Actin defect | | | increased level of actin | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 40170 | CG8103 | Mi-2 | 107204 | np | Effect on follicular epithelium morphology | | | further phenotype data in classification screen | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 40170 | CG8103 | Mi-2 | 107204 | np | No ovaries | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | GR00335-A | 40170 | FBgn0262519 | Mi-2 | HMS00301 | np | F1 lethal | | | F1 larval lethal | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 40170 | FBgn0262519 | Mi-2 | GL00318 | np | Agametic | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 40170 | FBgn0262519 | Mi-2 | HMC03329 | np | F1 lethal | | | F1 larval lethal | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 40170 | FBgn0262519 | np | np | sp | none | | | | | | | | | | | | | | | | | | | | 40170 | FBgn0262519 | np | np | -2.87 | Increased transposon expression | | | fold change: 3.96 | | | | | | | | | | | | | | | | GR00339-A-2 | 40170 | FBgn0262519 | Mi-2 | 10766 | 2 | Increased transposon expression | | | | piRNA pathway regulation (2) | A Genome-wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary piRNA Biogenesis in Drosophila | Muerdter et al. | 2013 | 23665228 | Tissue | traffic jam GAL4 | Gypsy and ZAM transposon expression | qPCR | VDRC (GD, KK) and TRiP | Selected genes | UAS-IR construct | Fold change | > | Following transposons were used for scoring: Gypsy for KK and ZAM for GD RNAi lines. TRIP reagents als TRIP_11111 dargestellt, Suchbarkeit? Developmental defect: wurden nicht gemessen, passt np als Score? | GR00367-S | 40170 | CG8103 | | 10766 | np | none | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 40170 | CG8103 | | 107204 | 0.28678228606758777 | none | | | Intermediate eggs/larvae level | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00379-A-2 | 40170 | CG8103 | | 107204 | np | Germarium defects in 40%; Egg chamber defects in 30%; No phenotype in 30% | | | | Defects in female germline | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | Vasa AND 1B1 expression | Immunofluorescence | VDRC | Selected genes | UAS-IR construct | np | np | Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00385-A | 40170 | | | 107204 | 0.2 | Low performer in olfactory memory formation | | | Final hit | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00388-A | 40170 | CG8103 | | BL33419 | | Lethal | | | ISC to EC differentiation | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 40170 | CG8103 | | GD10766 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |