| GR00002-A | 40444 | FBgn0000045 | Act79B | DRSC11604 | -0.82383536 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40444 | FBgn0000045 | Act79B | DRSC11604 | -2.438852219 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00013-A | 40444 | CG7478 | Act79B | np | np | Decreased Chlamydia caviae infection | | yes | | Chlamydia caviae infection | RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection. | Derre et al. | 2007 | 17967059 | Cell line | SL2 | Chlamydia MOMP and LPS expression and DNA content | Fluorescence | np | Genome-wide | dsRNA | Visual inspection | np | Additional information about secondary screens | GR00021-A-0 | 40444 | FBgn0000045 | | DRSC11604 | weak | ERK activation reduced | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 40444 | | | HFA11604 | 1.2 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 40444 | | | HFA11604 | 1.2 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 40444 | FBgn0000045 | Act79B | DRSC11604 | medium | Binucleate cells with microtubule extensions | | no | | | | | | | | | | | | | | | | | GR00034-A | 40444 | | Actin | np | 12 | Increased Dipt::lacZ protein expression | | | without lipopolysaccharide stimulation, CDRi | Imd pathway regulation | Functional dissection of an innate immune response by a genome-wide RNAi screen. | Foley and O'Farrell | 2004 | 15221030 | Cell line | S2 | Dipt::lacZ protein expression | Colorimetrics | Custom-made | Selected genes | dsRNA | Percentage LacZ-positive cells | Complex criteria | Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish) | GR00034-A | 40444 | | Actin | np | 22 | Increased Dipt::lacZ protein expression after lipopolysaccharide stimulation | | | with lipopolysaccharide stimulation, EDRi | Imd pathway regulation | Functional dissection of an innate immune response by a genome-wide RNAi screen. | Foley and O'Farrell | 2004 | 15221030 | Cell line | S2 | Dipt::lacZ protein expression | Colorimetrics | Custom-made | Selected genes | dsRNA | Percentage LacZ-positive cells | Complex criteria | Phenotype categories: decreased defense by RNAi (DDRi) genes, enhanced defense by RNAi (EDRi) genes, and constitutive defense by RNAi (CDRi) genes. Additional information about the primary genome-wide screen and secondary screens (double knockdown with Imd, Dredd or Relish) | GR00084-A-0 | 40444 | FBgn0000045 | CG7478 | np | np | C. trachomatis infection down | | no | | C. trachomatis infection | RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry. | Elwell et al. | 2008 | 18369471 | Cell line | S2 | C. trachomatis infection | High content (microscopy) | OpenBiosystems | | dsRNA | Visual inspection | np | | GR00130-A | 40444 | FBgn0000045 | Act79B | np | 1.5 - 2 | Increased mutant human huntingtin aggregation | | no | | Mutant human huntingtin aggregation | RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. | Doumanis et al. | 2009 | 19789644 | Cell line | BG2-c2 | Nhtt(62Q)EGFP aggregate number and size | Fluorescence | OpenBiosystems RNAi library | Selected genes | dsRNA | Z-score | Suppressor: < | | GR00134-A-1 | 40444 | CG7478 | Act79B | 9776 | np | Lethal | | yes | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-2 | 40444 | CG7478 | Act79B | np | np | Wing posture / locomotion / ability to fly defect or lethal / semi-lethal | | no | | Muscle morphogenesis and function (2) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | Custom-made | Selected genes | UAS-IR construct | rp | S19 > 0.5 | | GR00142-A-1 | 40444 | CG7478 | Act79B | 9776 | 1.78666666666666 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 40444 | CG7478 | | 9776 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00214-A-1 | 40444 | FBgn0000045 | Act79B | DRSC11604 | -1.52 | Decreased ERK phosphorylation after EGF stimulation | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 40444 | FBgn0000045 | Act79B | DRSC11604 | 0.43 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 40444 | FBgn0000045 | Act79B | DRSC11604 | 1.73 | Increased ERK phosphorylation after sSpitz and EGF stimulation | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 40444 | FBgn0000045 | Act79B | DRSC11604 | 0.94 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00216-A | 40444 | FBgn0000045 | Act79B | BKN32081 | -0.6 | none | | no | | Wnt proteins secretion | p24 proteins are required for secretion of Wnt ligands. | Buechling et al. | 2011 | 22094269 | Cell line | S2R+ | Wg pathway reporter | Luminescence | BKN | Cellular transporting machinery genes | dsRNA | Z-score | > 2.5 OR < -2.5 | Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4) | GR00218-S | 40444 | CG7478 | Act79B | AMB30628 | 0.16 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00238-S-1 | 40444 | CG7478 | Act79B | 7478R-2 | sp | Climbing defect | | yes | library: NIG | Muscle development and maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | Mef2-GAL4 | Climbing ability and viability | Negative geotaxis assay and visual inspection | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00238-S-1 | 40444 | CG7478 | Act79B | 102588 | sp | Embryo lethal | | yes | library: VDRC | Muscle development and maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | Mef2-GAL4 | Climbing ability and viability | Negative geotaxis assay and visual inspection | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00238-S-2 | 40444 | CG7478 | Act79B | 7478R-2 | sp | Climbing defect | | no | library: NIG | Muscle maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | TARGET Mef2-GAL4 | Climbing ability | Negative geotaxis assay | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00238-S-2 | 40444 | CG7478 | Act79B | 102588 | sp | Climbing defect | | no | library: VDRC | Muscle maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | TARGET Mef2-GAL4 | Climbing ability | Negative geotaxis assay | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00239-A-1 | 40444 | CG7478 | | 7478R | np | Decreased Chp protein expression | | no | not detectable Chp level | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00273-A-1 | 40444 | FBgn0000045 | Act79B | DRSC11604 | 3 | Effect on Polycomb (Pc) foci | | | Weakly expressed | Polycomb group (PcG) proteins regulation (1) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Pc-GFP protein localization | Fluorescence | DRSC | Genome-wide and transcription factors | dsRNA | Visual inspection | 1 | Additional phenotypic clustering screen available. | GR00273-A-2 | 40444 | FBgn0000045 | Act79B | DRSC11604 | np | Decreased Polycomb (Pc) foci intensity | | | Weakly expressed | Polycomb group (PcG) proteins regulation (2) | Identification of Regulators of the Three-Dimensional Polycomb Organization by a Microscopy-Based Genome-wide RNAi Screen | Gonzalez et al. | 2014 | 24703951 | Cell line | S2 | Polycomb-GFP protein localization | Fluorescence | DRSC | Selected genes | dsRNA | Visual inspection | np | | GR00284-A | 40444 | CG7478 | Act79B | 102588 | 0.7222796077759358 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 40444 | FBgn0000045 | Act79B | DRSC11604 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00335-A | 40444 | FBgn0000045 | Act79B | HMS00303 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 40444 | FBgn0000045 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 40444 | CG7478 | | 102588 | np | liquid clearance defect | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 40444 | CG7478 | | 102588 | 0.00994049614608318 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 40444 | CG7478 | | KK102588 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 40444 | CG7478 | | GD9776 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |