| GR00002-A | 40620 | FBgn0000340 | cno | DRSC12374 | 2.181716658 | none | | yes | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40620 | FBgn0051537 | CG31537 | DRSC12054 | -1.373737891 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40620 | FBgn0051537 | CG31537 | DRSC12054 | 1.288647117 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40620 | FBgn0000340 | cno | DRSC22980 | 2.12127873 | Increased lipid droplet area | | yes | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 40620 | FBgn0000340 | cno | DRSC22980 | 2.856155405 | Increased lipid droplet area | | yes | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00031-A-1 | 40620 | | | HFA12054 | 0.3 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-1 | 40620 | | cno | HFA12374 | 3 | Decreased cell number and viability | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 40620 | | | HFA12054 | -0.7 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 40620 | | cno | HFA12374 | 4.5 | Decreased cell number and viability | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00047-A-1 | 40620 | FBgn0000340 | cno | DRSC12374 | sp | Decreased horseradish peroxidase protein expression | | no | | Constitutive protein secretion and Golgi organization (1) | Functional genomics reveals genes involved in protein secretion and Golgi organization. | Bard et al. | 2006 | 16452979 | Cell line | S2 | Horseradish peroxidase protein expression | Luminescence | | Genome-wide | dsRNA | Z-score | < -1.5 | | | 40620 | FBgn0000340 | cno | LD24616 | sp | Increased G2 DNA content | | yes | library: DGC1 | | | | | | | | | | | | | | | | | 40620 | FBgn0000340 | cno | np | sp | none | | yes | library: DGC1 | | | | | | | | | | | | | | | | GR00048-A-2 | 40620 | FBgn0000340 | cno | np | 1.392 | none | | yes | | Cell size and cell-cycle regulation (2) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Proliferation and viability | Colorimetrics | | Selected genes | dsRNA | MTS metabolic activity | > 2.5 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-3 | 40620 | FBgn0000340 | cno | np | 437 | none | | yes | | Cell size and cell-cycle regulation (3) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Cell number | Fluorescence | | Selected genes | dsRNA | Average cell number per field | > 2 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-4 | 40620 | FBgn0000340 | cno | np | below threshold | none | | no | | Cell size and cell-cycle regulation (4) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Number of mitotic cells | Fluorescence | | Selected genes | dsRNA | Percentage | Visual inspection | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00062-A | 40620 | FBgn0000340 | cno | np | 2 | Decreased Ca2+ influx | | | multiple potential off-targets | Ca2+ channel regulation | CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. | Vig et al. | 2006 | 16645049 | Cell line | S2R+ | Ca2+ influx | Fluorescence | np | Genome-wide | dsRNA | Complex, sp | > | | GR00130-A | 40620 | FBgn0000340 | cNo | np | <= -2 | Decreased mutant human huntingtin aggregation | | no | | Mutant human huntingtin aggregation | RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. | Doumanis et al. | 2009 | 19789644 | Cell line | BG2-c2 | Nhtt(62Q)EGFP aggregate number and size | Fluorescence | OpenBiosystems RNAi library | Selected genes | dsRNA | Z-score | Suppressor: < | | GR00134-A-1 | 40620 | CG2534 | cno | 7769 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 40620 | CG2534 | cno | 7769 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 40620 | CG2534 | | 7769 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00139-A | 40620 | | cno | 7769 | sp | Lethal | | no | | Fly viability and adult morphology | A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. | Dietzl et al. | 2007 | 17625558 | Organism | Act5C-GAL4 | Adult morphology and viability | Visual inspection | Custom-made | Selected and random genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) | GR00142-A-1 | 40620 | CG2534 | cno | 7769 | 0.172839506172839 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 40620 | CG2534 | | 7769 | 2 | Notum malformation death | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00148-A | 40620 | FBgn0051537 | CG31537 | np | -2.09 | Decreased P-JNK protein expression with PGN | 15 min PGN induction | no | | PGN-induced dJNK phosphorylation | A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. | Bond and Foley | 2009 | 19893628 | Cell line | S2 | P-JNK protein expression | Fluorescence | Custom-made | Genome-wide | dsRNA | Z-score | Complex criteria | Only hits stored in GenomeRNAi | GR00170-A-1 | 40620 | | canoe | DRSC12374 | -3.2 | none | | no | decreased cell viability or Sanger collection sequence | HIF-dependent transcription (1) | Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia. | Dekanty et al. | 2010 | 20585616 | Cell line | S2 | Hypoxia inducible HRE reporter | Luminescence | DRSC 1.0 library | Genome-wide | dsRNA | Z-score | < | | GR00190-A-1 | 40620 | FBgn0000340 | cno | 7769 | -0.182 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00217-A-1 | 40620 | CG42312 | cno | np | medium | Decreased WgRluc and not changed sFluc protein expression | | no | | Wg pathway regulation (1) | A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. | Port et al. | 2011 | 21886182 | Cell line | S2R+ | WgRluc and sFluc protein expression | Luminescence | np | Genome-wide | dsRNA | Phenotype strength | Complex criteria | Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4) | GR00218-S | 40620 | CG31537 | cno | AMB29627 | 0.36 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00218-S | 40620 | CG2534 | cno | AMB25082 | 0.15 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 40620 | CG2534 | | 2534R | sp | Increased alpha1,6-fucose Chp glycosylation, increased mannose Chp glycosylation | | yes | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00239-A-2 | 40620 | CG2534 | | 2534R | np | none | | no | | Glycosylation regulation (2) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation | Luminescence | np | Selected genes | UAS-IR construct | p-value | < 0.005 | | GR00282-A | 40620 | CG42312 | cno | 102686 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 40620 | CG42312 | cno | 7769 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 40620 | CG42312 | cno | 102686 | 0.36802960545732866 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00308-A | 40620 | CG2534 | cno | np | 1.67 | Decreased cell aggregation | | | validated in secondary screen | Cadherin-mediated cell-cell adhesion | A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion | Toret et al. | 2014 | 24446484 | Cell line | S2 | Cell aggregation | Fluorescence | V2 RNAi library (Thermo Fisher Scientific) | Genome-wide | dsRNA | Visual inspection; average of 3 replicates; 0 | > 1.5 | Additional secondary screen available. S2 cells stably express DE-cadherin. | GR00311-A | 40620 | FBgn0259212 | cno | DRSC12054 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00311-A | 40620 | FBgn0024308 | Smr | DRSC12374 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 40620 | CG31537 | cno | 102686 | np | Effect on follicular epithelium morphology | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 40620 | CG31537 | cno | 102686 | np | none | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | GR00335-A | 40620 | FBgn0259212 | cno | HMS00239 | np | Embryonic lethal | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 40620 | FBgn0259212 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 40620 | CG42312 | | 7769 | np | lethal or adult morphology phenotype | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 40620 | CG31537 | | 102686 | -0.29665041613686854 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00385-A | 40620 | | | 102686 | 0.18 | Low performer in olfactory memory formation | | | | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00388-A | 40620 | CG42312 | | BL33367 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 40620 | CG2534 | | GD7769 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 40620 | CG31537 | | KK102686 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |