RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:41085
  • Symbol:neur
  • Description:neuralized
DataSource: http://genomernai.dkfz.de/v16/genedetails/41085

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 41085 FBgn0002932 neur DRSC17013 -0.094222078 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 41085 FBgn0002932 neur DRSC17013 -0.817651832 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00012-A-1 41085 CG11988 neur np np Increased viability with doxorubicin yes Caspase activation in apoptosis (1) - combinatorial effect with doxorubicin A genome-wide RNAi screen reveals multiple regulators of caspase activation. Yi et al. 2007 17998402 Cell line Kc167 Viability (synthetic lethal) Luminescence HFA Genome-wide dsRNA Z-score Complex criteria
GR00012-A-2 41085 CG11988 neur np np none yes Caspase activation in apoptosis (2) - combinatorial effect with doxorubicin A genome-wide RNAi screen reveals multiple regulators of caspase activation. Yi et al. 2007 17998402 Cell line Kc167 Caspase 3/7 activity Luminescence HFA Selected genes dsRNA p-value < 0.05
GR00012-A-3 41085 CG12284, CG11988 diap1, neur np np Increased viability with diap1 RNAi no Caspase activation in apoptosis (3) - combinatorial effect with diap1 RNAi A genome-wide RNAi screen reveals multiple regulators of caspase activation. Yi et al. 2007 17998402 Cell line Kc167 Viability (synthetic lethal) Luminescence HFA Selected genes dsRNA p-value < 0.05
GR00031-A-1 41085 HFA17013 0.7 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 41085 HFA17013 0.1 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
41085 FBgn0002932 neur LD45505 sp none no library: DGC1
41085 CG11988 neur np np Heart tube assembly defects, heart patterning defects no
GR00134-A-1 41085 CG11988 neur 10662 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 41085 CG11988 neur 10662 -0.34 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 41085 CG11988 10662 1 none no Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00142-A-1 41085 CG11988 neur 10662 -0.661971830985915 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-1 41085 CG11988 neur 47B8 no phenotype none temperature: 25 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 41085 CG11988 neur 47B8 no phenotype none temperature: 29 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 41085 CG11988 neur 47B8 T3 no phenotype none temperature: 18 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 41085 CG11988 neur 47B8 T3 no phenotype none temperature: 25 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 41085 CG11988 neur 47B8 T3 no phenotype none temperature: 29 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 41085 CG11988 neur 47B8 T4 no phenotype none temperature: 18 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 41085 CG11988 neur 47B8 T4 no phenotype none temperature: 25 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 41085 CG11988 neur 47B8 T4 no phenotype none temperature: 29 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 T3 no phenotype none temperature: 18 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 T4 no phenotype none temperature: 18 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 medium to strong Gain of bristles, duplic ated hairs, both head and notum temperature: 29 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 T3 rare, weak Mild gain of bristles on head temperature: 29 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 T4 rare, weak Mild gain of bristles on head, M only temperature: 29 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 T3 no phenotype none temperature: 25 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 T4 no phenotype none temperature: 25 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 41085 CG11988 neur 47B8 no phenotype none temperature: 25 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 T3 no phenotype none temperature: 18 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 T3 no phenotype none temperature: 25 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 T3 medium to strong Gain of bristles, duplicated hairs temperature: 29 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 T4 no phenotype none temperature: 18 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 T4 no phenotype none temperature: 25 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 T4 rare, weak Mild gain of bristles on head and notum temperature: 29 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 no phenotype none temperature: 25 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 41085 CG11988 neur 47B8 medium to strong Gain of bristles, duplicated hairs temperature: 29 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-4 41085 CG11988 10662 sp Gain of bristles, overproliferation, lateral inhibition yes not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-5 41085 CG11988 10662 np none no Notch pathway regulation (5) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue MS1096-GAL4 Wing morphology and viability Visual inspection np Selected genes UAS-IR construct Visual inspection np
GR00190-A-1 41085 FBgn0002932 neur 10662 -0.09 none no Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00218-S 41085 CG11988 neur AMB19775 -0.98 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00239-A-1 41085 CG11988 11988R sp none no Glycosylation regulation (1) Identification of genes required for neural-specific glycosylation using functional genomics. Yamamoto-Hino et al. 2010 21203496 Tissue GMR-GAL4 Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability Luminescence np Selected genes UAS-IR construct Z-score > 3
GR00282-A 41085 CG11988 neur 108239 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00282-A 41085 CG11988 neur 10662 np none piRNA pathway regulation The Genetic Makeup of the Drosophila piRNA Pathway Handler 2013 23665231 Tissue tj-GAL4 Gypsy transposon expression Microscopy VDRC Selected genes UAS-IR construct Visual inspection np See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data
GR00284-A 41085 CG11988 neur 108239 -0.2255549566282894 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 41085 FBgn0002932 neur DRSC17013 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 41085 CG11988 neur 108239 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00326-A 41085 FBgn0002932 neur 11988 np none Synapse formation and maintenance A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance Valakh et al. 2012 22542760 Tissue Elav-Gal4 Neuromuscular junction morphology Fluorescence VDRC Selected genes UAS-IR construct Visual inspection np For further phenotype explanations sp.
GR00335-A 41085 FBgn0002932 neur GL00334 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 41085 FBgn0002932 neur JF02048 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
41085 FBgn0002932 np np sp none
GR00367-S 41085 CG11988 10662 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 41085 CG11988 108239 -0.11688868452262698 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A 41085 108239 0.35400688409710457 none Regulation of olfactory memory formation Identification of genes that promote or inhibit olfactory memory formation in Drosophila. Walkinshaw et al. 2015 25644700 Organism Nsyb-GAL4 T-maze performance Visual inspection VDRC Selected genes UAS-IR construct Performance index, sp High performer in olfactory memory formation: > Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A 41085 CG11988, CG31429 BL26023 Lethal excess of ISC or EB Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 41085 CG11988 GD10662 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.