| GR00002-A | 41549 | FBgn0038063 | Octbeta2R | DRSC15350 | 0.196318361 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 41549 | FBgn0038063 | Octbeta2R | DRSC15350 | -1.197815469 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 41549 | FBgn0038063 | Octbeta2R | DRSC16165 | 0.461238653 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 41549 | FBgn0038063 | Octbeta2R | DRSC16165 | 0.136525947 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00005-A-0 | 41549 | FBgn0038063 | | DRSC16165 | strong | Centrosome clustering defects | | no | | Multipolar division | Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. | Kwon et al. | 2008 | 18662975 | Cell line | S2 | Centrosome clustering | High content (microscopy) | DRSC | | dsRNA | Visual inspection | np | | GR00031-A-1 | 41549 | | | HFA16165 | 0.7 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-1 | 41549 | | | HFA15350 | 0.5 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 41549 | | | HFA16165 | -0.3 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 41549 | | | HFA15350 | -0.9 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00049-A-0 | 41549 | FBgn0038063 | | DRSC16165 | 2 | Decreased NFAT translocation | | no | | NFAT signaling | A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT. | Gwack et al. | 2006 | 16511445 | Cell line | S2R+ | NFAT translocation | High content (microscopy) | DRSC | | dsRNA | Visual inspection | np | | GR00134-A-1 | 41549 | CG33976 | Octbeta2R | 8486 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 41549 | CG33976 | Octbeta2R | 1765 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 41549 | CG18553 | Octbeta2R | 8486 | -1.17 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00142-A-1 | 41549 | CG18553 | CG18553 | 8486 | 0.520547945205479 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 41549 | CG33976 | | 1765 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 41549 | CG33976 | | 8486 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00190-A-1 | 41549 | FBgn0038063 | Octbeta2R | 8486 | 0.231 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00214-A-1 | 41549 | FBgn0038063 | Octbeta2R | DRSC15350 | -1.57 | Decreased ERK phosphorylation after EGF stimulation | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 41549 | FBgn0038063 | Octbeta2R | DRSC15350 | -1.51 | Decreased ERK phosphorylation after EGF stimulation | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 41549 | FBgn0038063 | Octbeta2R | DRSC15350 | -1.67 | Decreased ERK phosphorylation after sSpitz and EGF stimulation | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 41549 | FBgn0038063 | Octbeta2R | DRSC15350 | -0.52 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 41549 | CG18553 | | AMB21021 | 1.06 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00218-S | 41549 | CG6989 | | AMB21097 | -1.76 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00284-A | 41549 | CG33976 | Octbeta2R | 104050 | 0.10074473249489571 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 41549 | FBgn0038063 | Octbeta2R | DRSC15350 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00311-A | 41549 | FBgn0038063 | Octbeta2R | DRSC16165 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 41549 | CG33976 | Octbeta2R | 104524 | np | none | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00335-A | 41549 | FBgn0038063 | Octbeta2R | HMS01151 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 41549 | FBgn0038063 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 41549 | CG33976 | | 8486 | np | none | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 41549 | CG33976 | | 104050 | -0.31886528094654265 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00385-A | 41549 | | | 104050 | 0.18751544562244904 | Low performer in olfactory memory formation | | | | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00385-A | 41549 | | | 104524 | 0.42 | none | | | | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00385-A | 41549 | | | 106912 | 0.41 | none | | | | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00388-A | 41549 | CG33976 | | KK104524 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 41549 | CG33976 | | BL34673 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |