| GR00002-A | 42239 | FBgn0027841 | CstF-64 | DRSC16616 | -0.013116338 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 42239 | FBgn0027841 | CstF-64 | DRSC16616 | -2.121143308 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00031-A-1 | 42239 | | | HFA16616 | -0.6 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 42239 | | | HFA16616 | -0.6 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00035-A | 42239 | CG7697 | CstF-64 | JP111 | sp | none | | no | | Alternative splicing | Identification of alternative splicing regulators by RNA interference in Drosophila. | Park et al. | 2004 | 15492211 | Cell line | S2 | dAdar, para and Dscam mRNA expression | Reverse transcription PCR and autoradiography | Custom-made | RNA-binding protein genes and spliceosomal genes | dsRNA | np | Alternative exon splicing for > | | | 42239 | FBgn0027841 | CstF-64 | RE27227 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | GR00092-A | 42239 | FBgn0027841 | CG7697 | RDM1190-7301397 | np | none | | no | | Microtubule assembly and organization | Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. | Ducat et al. | 2008 | 18463163 | Cell line | S2 | DNA content, alpha-tubulin and CP309 protein expression | Fluorescence | OpenBiosystems Drosophila RNAi Library 1.0 | Mitotic regulator genes | dsRNA | Complex, sp | Complex criteria | | GR00134-A-1 | 42239 | CG7697 | CstF-64 | 21045 | np | Lethal | | no | late pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 42239 | CG7697 | CstF-64 | 21045 | -0.14 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 42239 | CG7697 | | 21045 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 42239 | CG7697 | CstF-64 | 21045 | 0.14516129032258 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 42239 | CG7697 | | 21045 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00183-A | 42239 | FBgn0027841 | CstF-64 | 21045 | sp | GFP aggregates number, GFP aggregates size, neuroblast less large cells, ganglion mother cell shorter lineages, neuroblast loss, neuroblast loss with unchanged neuroblast cell size, neuroblast underproliferation, lethal | | no | | Self-renewal and differentiation in neural stem cells | Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. | Neumueller et al. | 2011 | 21549331 | Tissue | insc-GAL4 | Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability | Fluorescence | np | Genome-wide | UAS-IR construct | Phenotype strength | > 1 | Additional information about a secondary screen (KK library) | GR00185-A | 42239 | CG7697 | CstF64 | np | 75 | none | | no | 20 min heat shock | Pol II elongation | Spt6 enhances the elongation rate of RNA polymerase II in vivo. | Ardehali et al. | 2009 | 19279664 | Cell line | S2 | Hsp70 mRNA expression | Real-time qPCR | Custom-made | Transcription factors | dsRNA | Percentage | < | | GR00189-A-1 | 42239 | FBgn0027841 | CstF-64 | BKN27450 | np | Upregulation of Notch pathway | | no | | Notch pathway regulation (1) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Cell line | S2 | Notch pathway reporter | Luminescence | BKN | Genome-wide | dsRNA | Complex, sp | Complex criteria | | GR00190-A-1 | 42239 | FBgn0027841 | CstF-64 | 21045 | -0.457 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00214-A-1 | 42239 | FBgn0027841 | CstF-64 | DRSC29486 | 1.08 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 42239 | FBgn0027841 | CstF-64 | DRSC29486 | -0.81 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 42239 | FBgn0027841 | CstF-64 | DRSC29486 | 0.56 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 42239 | FBgn0027841 | CstF-64 | DRSC29486 | 1.89 | Increased ERK phosphorylation after sSpitz and EGF stimulation | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00217-A-1 | 42239 | CG7697 | CstF-64 | np | weak | Decreased WgRluc and not changed sFluc protein expression | | no | | Wg pathway regulation (1) | A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. | Port et al. | 2011 | 21886182 | Cell line | S2R+ | WgRluc and sFluc protein expression | Luminescence | np | Genome-wide | dsRNA | Phenotype strength | Complex criteria | Phenotype strength categories: strong: both duplicates 2 standard deviations (SD) below plate mean; medium: both duplicates 1 SD below plate mean; weak: one duplicate 2 SD below plate mean, the other not significantly changed; additional information about secondary screens (hhGAL4 and eyGAL4 GMR–GAL4) | GR00218-S | 42239 | CG7697 | | AMB18852 | -2.02 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 42239 | CG7697 | | 7697R | sp | Increased mannose Chp glycosylation | | yes | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00239-A-2 | 42239 | CG7697 | | 7697R | np | none | | no | | Glycosylation regulation (2) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression and alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation | Luminescence | np | Selected genes | UAS-IR construct | p-value | < 0.005 | | GR00282-A | 42239 | CG7697 | CstF-64 | 21045 | np | Increased transposon expression | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 42239 | CG7697 | CstF-64 | 21045 | 0.401894931491516 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00308-A | 42239 | CG7697 | CstF-64 | np | 2 | Decreased cell aggregation | | | | Cadherin-mediated cell-cell adhesion | A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion | Toret et al. | 2014 | 24446484 | Cell line | S2 | Cell aggregation | Fluorescence | V2 RNAi library (Thermo Fisher Scientific) | Genome-wide | dsRNA | Visual inspection; average of 3 replicates; 0 | > 1.5 | Additional secondary screen available. S2 cells stably express DE-cadherin. | GR00311-A | 42239 | FBgn0027841 | CstF-64 | DRSC16616 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00330-A | 42239 | | CstF-64 | DRSC16616 | np | Decreased androgen receptor (AR) reporter expression | | | | Androgen receptor (AR) regulation | A genome-wide RNA interference screen identifies new regulators of androgen receptor function in prostate cancer cells | Imberg-Kazdan et al. | 2013 | 23403032 | Cell line | S2R+ | Androgen receptor (AR) reporter | Luminescence | Whole Genome Drosophila Library (DRSC 2.0) | Genome-wide | dsRNA | Complex, sp | Complex criteria | Reporter expression was measured after stimulation with 10 nM of synthetic androgen. Results from the primary screen have been deposited with the Drosophila RNAi Screening Center (DRSC) (http://www.flyrnai. org) under the title ‘‘Factors affecting human androgen receptor transcriptional activation’’. | | 42239 | FBgn0027841 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 42239 | CG7697 | | 21045 | np | liquid clearance defect | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 42239 | CG7697 | | 21045 | -0.2504691766867558 | none | | | Intermediate eggs/larvae level | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00379-A-2 | 42239 | CG7697 | | 21045 | np | none | | | | Defects in female germline | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | Vasa AND 1B1 expression | Immunofluorescence | VDRC | Selected genes | UAS-IR construct | np | np | Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 42239 | CG7697 | | GD21045 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |