| GR00002-A | 42313 | FBgn0000463 | Dl | DRSC16648 | 0.131680767 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 42313 | FBgn0000463 | Dl | DRSC16648 | 0.006979434 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00021-A-0 | 42313 | FBgn0000463 | | DRSC16648 | weak | ERK activation increased | | no | | ERK signaling | A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. | Friedman et al. | 2006 | 17086199 | Cell line | S2R+ | ERK signaling | Fluorescence | DRSC | | dsRNA | Weak, moderate, strong | np | | GR00031-A-1 | 42313 | | | HFA16648 | -0.4 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 42313 | | | HFA16648 | 0.1 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 42313 | FBgn0000463 | Dl | LD19584 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 42313 | CG3619 | Dl | np | np | Defects in nervous system development | | no | “Known” phenotype | | | | | | | | | | | | | | | | | 42313 | CG3619 | Dl | np | np | Heart patterning defects | | no | | | | | | | | | | | | | | | | | GR00083-A | 42313 | CG3619 | Dl | np | 66.61 | none | | no | | Brucella melitensis infection | RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication. | Qin et al. | 2008 | 18654626 | Cell line | S2 | Intracellular bacterial GFP protein expression | Fluorescence | Drosophila RNAi Library Release 1.0 | Selected genes | dsRNA | Relative infection | > 2 standard deviations below untreated control | Additional information about secondary screens (gentamicin protection assays) | GR00128-A-2 | 42313 | FBgn0000463 | CG3619 | DRSC38075 | sp | none | | no | | Combinatorial effect with methyl methanesulphonate (2) | A network of conserved damage survival pathways revealed by a genomic RNAi screen. | Ravi et al. | 2009 | 19543366 | Cell line | Kc167 | Viability (synthetic lethal) | Luminescence | Version 1 | Selected genes | dsRNA | rp | rp | | GR00134-A-1 | 42313 | CG3619 | Dl | 37288 | np | Weak flyer | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 42313 | CG3619 | Dl | 3720 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 42313 | CG3619 | Dl | 37287 | np | Lethal | | no | late pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 42313 | CG3619 | Dl | 27187 | np | Lethal | | no | late pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 42313 | CG3619 | Dl | 3720 | 1.39 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 42313 | CG3619 | Dl | 27187 | 2.97 | none | | yes | potential off-target effects | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 42313 | CG3619 | Dl | 37287 | 8.18 | Increased noxious heat avoidance | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-1 | 42313 | CG3619 | Dl | 37288 | 6.85 | Increased noxious heat avoidance | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-2 | 42313 | CG3619 | Dl | 37287 | 31.33333333 | none | | no | | Heat nociception (2) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Temperature-induced paralysis | Exposure to 46 oC | np | Selected genes | UAS-IR construct | Mean kinetics | Significant increase | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00135-A-2 | 42313 | CG3619 | Dl | 37288 | 32.33333333 | none | | no | | Heat nociception (2) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Temperature-induced paralysis | Exposure to 46 oC | np | Selected genes | UAS-IR construct | Mean kinetics | Significant increase | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 42313 | CG3619 | | 3720 | 1 | none | | yes | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00138-A-1 | 42313 | CG3619 | | 37288 | 0.88 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00138-A-1 | 42313 | CG3619 | | 27187 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00138-A-2 | 42313 | CG3619 | | 3720 | 2.73 | none | | no | | Heart development and function (2) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00139-A | 42313 | | Dl | 3720 | sp | Lethal | | no | | Fly viability and adult morphology | A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. | Dietzl et al. | 2007 | 17625558 | Organism | Act5C-GAL4 | Adult morphology and viability | Visual inspection | Custom-made | Selected and random genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) | GR00139-A | 42313 | | Dl | 37287 | sp | Lethal | | no | | Fly viability and adult morphology | A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. | Dietzl et al. | 2007 | 17625558 | Organism | Act5C-GAL4 | Adult morphology and viability | Visual inspection | Custom-made | Selected and random genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) | GR00139-A | 42313 | | Dl | 37288 | sp | Lethal | | no | | Fly viability and adult morphology | A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. | Dietzl et al. | 2007 | 17625558 | Organism | Act5C-GAL4 | Adult morphology and viability | Visual inspection | Custom-made | Selected and random genes | UAS-IR construct | Phenotype strength | np | Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4) | GR00142-A-1 | 42313 | CG3619 | Dl | 37287 | 1.59615384615384 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T1 | no phenotype | none | temperature: 18 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T1 | lethal | Adults die in food, escapers have no phenotype | temperature: 25 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T1 | lethal | Lethal, pharate adults with gain of bristles | temperature: 25 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T1 | rare, weak | Weak gain of bristles, lethal | temperature: 29 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T1 | rare, weak | Weak gain of bristles, lethal | temperature: 29 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T2 | no phenotype | none | temperature: 18 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T2 | no phenotype | none | temperature: 25 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-1 | 42313 | CG3619 | Dl | 2B7 T2 | lethal | Lethal | temperature: 29 | yes | | Notch pathway regulation (1) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | sca-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T2 | lethal | Adults die in food | temperature: 29 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T1 | lethal | Adults die in food, gain of bristles | temperature: 29 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T2 | lethal | Adults die in food, gain of bristles | temperature: 29 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T1 | lethal | Adults die in food, gain of bristles | temperature: 25 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T2 | medium to strong | Gain of bristles | temperature: 25 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Gain of bristles, head and notum | temperature: 25 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Gain of bristles, m only | temperature: 18 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T2 | medium to strong | Gain of bristles, m only | temperature: 18 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T2 | rare, weak | Gain of bristles, m only | temperature: 18 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T1 | lethal | Lethal | temperature: 29 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-2 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Strong gain of bristles, m only | temperature: 18 | yes | | Notch pathway regulation (2) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | fz3-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Gain of bristles, m only | temperature: 18 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Strong gain of bristles, m only | temperature: 18 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Gain of bristles, m only | temperature: 25 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Strong gain of bristles | temperature: 25 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T1 | medium to strong | Strong gain of bristles | temperature: 29 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T2 | medium to strong | Gain of bristles, m only | temperature: 18 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T2 | medium to strong | Gain of bristles | temperature: 25 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T2 | medium to strong | Strong gain of bristles | temperature: 29 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-3 | 42313 | CG3619 | Dl | 2B7 T2 | np | Bad cross, phenotype not checked | temperature: 18 | yes | | Notch pathway regulation (3) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 42313 | CG3619 | | 37287 | sp | Gain of bristles, loss of bristles, lateral inhibition | | yes | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 42313 | CG3619 | | 37288 | sp | Gain of bristles, loss of bristles, notum malformation death, lateral inhibition | | yes | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 42313 | CG3619 | | 27187 | sp | Gain of bristles, loss of bristles, lateral inhibition | | yes | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 42313 | CG3619 | | 3720 | sp | Gain of bristles, loss of bristles, lateral inhibition | | yes | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-5 | 42313 | CG3619 | | 3720 | np | Wing margin notching, broad delta-like veins | | yes | | Notch pathway regulation (5) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | MS1096-GAL4 | Wing morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Visual inspection | np | | GR00144-A-5 | 42313 | CG3619 | | 27187 | np | Wing margin notching, broad delta-like veins, thin wings | | yes | | Notch pathway regulation (5) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | MS1096-GAL4 | Wing morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Visual inspection | np | | GR00144-A-5 | 42313 | CG3619 | | 37288 | np | Wing margin notching, broad delta-like veins, wrinkled, blistered wings | | yes | | Notch pathway regulation (5) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | MS1096-GAL4 | Wing morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Visual inspection | np | | GR00144-A-5 | 42313 | CG3619 | | 37287 | np | Wing margin notching, broad delta-like veins, wrinkled, blistered wings | | yes | | Notch pathway regulation (5) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | MS1096-GAL4 | Wing morphology and viability | Visual inspection | np | Selected genes | UAS-IR construct | Visual inspection | np | | GR00144-A-6 | 42313 | CG3619 | | 27187 | np | none | | yes | | Notch pathway regulation (6) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | ptc-GAL4 | Su(H) protein expression | X-gal | np | Selected genes | UAS-IR construct | Visual inspection | np | | GR00144-A-6 | 42313 | CG3619 | | 37288 | np | none | | yes | | Notch pathway regulation (6) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | ptc-GAL4 | Su(H) protein expression | X-gal | np | Selected genes | UAS-IR construct | Visual inspection | np | | GR00144-A-6 | 42313 | CG3619 | | 3720 | np | none | | yes | | Notch pathway regulation (6) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | ptc-GAL4 | Su(H) protein expression | X-gal | np | Selected genes | UAS-IR construct | Visual inspection | np | | GR00144-A-7 | 42313 | CG3619 | | 27187 | np | Too many sensory organ precursor cells | | no | | Notch pathway regulation (7) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | Number and size of sensory organ precursor cells | Fluorescence | np | Selected genes | UAS-IR construct | Cell number and size | np | | GR00144-A-7 | 42313 | CG3619 | | 37287 | np | Too many sensory organ precursor cells | | no | | Notch pathway regulation (7) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | Number and size of sensory organ precursor cells | Fluorescence | np | Selected genes | UAS-IR construct | Cell number and size | np | | GR00144-A-7 | 42313 | CG3619 | | 37288 | np | Too many sensory organ precursor cells | | no | | Notch pathway regulation (7) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | Number and size of sensory organ precursor cells | Fluorescence | np | Selected genes | UAS-IR construct | Cell number and size | np | | GR00148-A | 42313 | FBgn0000463 | Dl | np | 2.84 | Increased P-JNK protein expression with PGN | 15 min PGN induction | no | | PGN-induced dJNK phosphorylation | A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. | Bond and Foley | 2009 | 19893628 | Cell line | S2 | P-JNK protein expression | Fluorescence | Custom-made | Genome-wide | dsRNA | Z-score | Complex criteria | Only hits stored in GenomeRNAi | GR00148-A | 42313 | FBgn0000463 | Dl | np | 3.4 | Increased P-JNK protein expression with PGN | 60 min PGN induction | no | | PGN-induced dJNK phosphorylation | A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. | Bond and Foley | 2009 | 19893628 | Cell line | S2 | P-JNK protein expression | Fluorescence | Custom-made | Genome-wide | dsRNA | Z-score | Complex criteria | Only hits stored in GenomeRNAi | GR00189-A-3 | 42313 | FBgn0000463 | Dl | np | np | Only viable with Notch | | no | | Notch pathway regulation (3) | A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. | Saj et al. | 2010 | 20493818 | Tissue | GMR-GAL4 | Notch pathway reporter | Fluorescence | Notch pathway component enriched RNAi library | Selected genes | UAS-IR construct | Average fluorescent intensity | Complex criteria | | GR00190-A-1 | 42313 | FBgn0000463 | Dl | 37287 | 0.461 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00214-A-1 | 42313 | FBgn0000463 | Dl | DRSC16648 | 0.28 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 42313 | FBgn0000463 | Dl | DRSC16648 | 0.47 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 42313 | FBgn0000463 | Dl | DRSC16648 | 0.41 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 42313 | FBgn0000463 | Dl | DRSC16648 | -0.17 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00282-A | 42313 | CG3619 | Dl | 27187 | np | none | | | not expressed | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 42313 | CG3619 | Dl | 3720 | np | none | | | not expressed | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 42313 | CG3619 | Dl | 109491 | -1.2550375226376946 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 42313 | FBgn0000463 | Dl | DRSC16648 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 42313 | CG3619 | Dl | 109491 | np | none | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00323-A-1 | 42313 | FBgn0000463 | Dl | 3720 | np | none | | | | Drosophila eye development | The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development | Marinho et al. | 2013 | 23416177 | Tissue | eyeless-GAL4 | Retina size | Microscopy | VDRC, NIG and TRiP | Selected genes | UAS-IR construct | Visual inspection | np | Single RNAi screen; For double RNAi screen see "Viriato (Vito) interactions during eye development | GR00323-A-2 | 42313 | FBgn0000463 | Dl | 3720 | np | Medium vito interaction | | | | Viriato (Vito) interactions during eye development | The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development | Marinho et al. | 2013 | 23416177 | Tissue | eyeless-Gal4 | Retina size | Microscopy | VDRC, NIG and TRiP | Selected genes | UAS-IR construct | Genetic interaction score | np | Double RNAi screen; vito knockdown was archieved by expression of UAS-vitoRNAiKK in the female flies. For single RNAi screen see "Drosophila eye development | GR00335-A | 42313 | FBgn0000463 | Dl | GL00520 | np | Oogenesis defect | | | late oogenesis defect | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 42313 | FBgn0000463 | Dl | JF02867 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 42313 | FBgn0000463 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 42313 | CG3619 | | 3720 | np | lethal or adult morphology phenotype | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 42313 | CG3619 | | 109491 | -0.10465353929884305 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00385-A | 42313 | | | 109491 | 0.338671329760962 | none | | | | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00388-A | 42313 | CG3619 | | BL28032 | | Lethal | | | excess of ISC or EB | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 42313 | CG3619 | | GD37287 | | Lethal | | | excess of ISC or EB | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 42313 | CG3619 | | GD27187 | | none | | | excess of ISC or EB | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |