GR00002-A | 42537 | FBgn0004862 | bap | DRSC16908 | -0.408707498 | none | | yes | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 42537 | FBgn0004862 | bap | DRSC16908 | -0.618940411 | none | | yes | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00031-A-1 | 42537 | | | HFA16908 | -0.3 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 42537 | | | HFA16908 | 0 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00065-A | 42537 | CG7902 | bap | bap | np | none | | no | | Dendrite pattern formation | Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites. | Parrish et al. | 2006 | 16547170 | Tissue | GAL4221 | mCD8 protein expression | Fluorescence | Custom-made | Transcription factors | UAS-IR construct | np | Phenotypes in multiple blind tests | Additional information about secondary screens | GR00238-S-1 | 42537 | CG7902 | bagpipe | 101354 | sp | Pupal lethal | | yes | library: VDRC | Muscle development and maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | Mef2-GAL4 | Climbing ability and viability | Negative geotaxis assay and visual inspection | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00238-S-2 | 42537 | CG7902 | bagpipe | 101354 | sp | none | | no | library: VDRC | Muscle maintenance | The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance. | Perkins et al. | 2014 | 25977760 | Tissue | TARGET Mef2-GAL4 | Climbing ability | Negative geotaxis assay | np | Selected genes | UAS-IR construct | Complex, sp | Complex criteria | Author-submitted data. Published in Scientific Data 1, Article number: 140002. | GR00296-A | 42537 | | Bap | np | -11.14 | Decreased Sindbis virus (SINV) infection | | | prim. screen rep. B: -7.02; sec. val. screen: -1.97 | Sindbis virus (SINV) infection | Genome-wide RNAi Screen Identifies SEC61A and VCP as Conserved Regulators of Sindbis Virus Entry | Panda et al. | 2013 | 24332855 | Cell line | DL1 | Sindbis virus (SINV) GFP protein expression | Fluorescence | Ambion | Genome-wide | dsRNA | Z-score | < -2 OR > 2 | Primary screen performed in duplicate. Z-scores from replicate B and from secondary validation screen are shown in the comment field. Recombinant SINV (HRsp) expressing GFP was used for infection. | GR00311-A | 42537 | FBgn0004862 | bap | DRSC16908 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 42537 | CG7902 | bap | 101354 | np | none | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00335-A | 42537 | FBgn0004862 | bap | JF02404 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 42537 | FBgn0004862 | np | np | sp | none | | | | | | | | | | | | | | | | | | | | 42537 | np | np | HFA16908 | 1.42 | none | | | fold change: 0.51 | | | | | | | | | | | | | | | | GR00367-S | 42537 | CG7902 | | 101354 | np | none | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00388-A | 42537 | CG7902 | | KK101354 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 42537 | CG7902 | | BL27061 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |