| GR00002-A | 42807 | FBgn0039111 | Plip | DRSC14235 | -0.184457054 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 42807 | FBgn0039111 | Plip | DRSC14235 | 0.530425435 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00005-A-0 | 42807 | FBgn0039111 | | DRSC14235 | weak | Centrosome clustering defects | | no | | Multipolar division | Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes. | Kwon et al. | 2008 | 18662975 | Cell line | S2 | Centrosome clustering | High content (microscopy) | DRSC | | dsRNA | Visual inspection | np | | GR00031-A-1 | 42807 | | | HFA14235 | 0.4 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 42807 | | | HFA14235 | 0.1 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 42807 | FBgn0039111 | CG10371 | RE13143 | sp | none | | no | library: DGC2 | | | | | | | | | | | | | | | | | 42807 | FBgn0039111 | Plip | CT29124 | sp | none | | no | library: PHOSPHO | | | | | | | | | | | | | | | | GR00049-A-0 | 42807 | FBgn0039111 | | DRSC14235 | 3 | Decreased NFAT translocation | | no | | NFAT signaling | A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT. | Gwack et al. | 2006 | 16511445 | Cell line | S2R+ | NFAT translocation | High content (microscopy) | DRSC | | dsRNA | Visual inspection | np | | GR00134-A-1 | 42807 | CG10371 | Plip | 47624 | np | Weak flyer | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 42807 | CG10371 | Plip | 47623 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 42807 | CG10371 | Plip | 47623 | 0.18 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 42807 | CG10371 | | 47623 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 42807 | CG10371 | Plip | 30698 | 0.028169014084507 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00142-A-1 | 42807 | CG10371 | Plip | 47624 | 0.171875 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 42807 | CG10371 | | 30698 | sp | Bristle morphology defects, planar polarity defects, notum malformation death | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00183-A | 42807 | FBgn0039111 | Plip | 47624 | sp | GFP aggregates number, GFP aggregates size, neuroblast larger cell size, neuroblast less large cells, neuroblast cell shape, ganglion mother cell shorter lineages, ganglion mother cell shape, neuroblast loss, neuroblast loss with larger neuroblast cell si | | no | | Self-renewal and differentiation in neural stem cells | Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. | Neumueller et al. | 2011 | 21549331 | Tissue | insc-GAL4 | Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability | Fluorescence | np | Genome-wide | UAS-IR construct | Phenotype strength | > 1 | Additional information about a secondary screen (KK library) | GR00190-A-1 | 42807 | FBgn0039111 | Plip | 30698 | -0.658 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00190-A-1 | 42807 | FBgn0039111 | Plip | 47624 | 1.196 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00191-A-1 | 42807 | CG10371 | Plip | np | np | none | | yes | | Blood cell homeostasis regulation (1) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-2 | 42807 | CG10371 | Plip | np | np | none | | no | | Blood cell homeostasis regulation (2) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00202-A | 42807 | FBgn0039111 | Plip | np | sp | none | | no | | Vaccinia virus infection | A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. | Moser et al. | 2010 | 20585561 | Cell line | DL1 | Virally encoded beta-galactosidase protein expression | Fluorescence | Custom-made | Kinases, phosphatases and selected genes | dsRNA | Z-score | < -2 in both duplicates | Author-reviewed data | GR00218-S | 42807 | CG10371 | | AMB32509 | 0.81 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 42807 | CG10371 | | 10371R | sp | none | | no | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00282-A | 42807 | CG10371 | Plip | 47623 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 42807 | CG10371 | Plip | 30698 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 42807 | CG10371 | Plip | 104774 | 0.2099439018621304 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00308-A | 42807 | CG10371 | CG10371 | np | 2 | Decreased cell aggregation | | | validated in secondary screen | Cadherin-mediated cell-cell adhesion | A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion | Toret et al. | 2014 | 24446484 | Cell line | S2 | Cell aggregation | Fluorescence | V2 RNAi library (Thermo Fisher Scientific) | Genome-wide | dsRNA | Visual inspection; average of 3 replicates; 0 | > 1.5 | Additional secondary screen available. S2 cells stably express DE-cadherin. | GR00311-A | 42807 | FBgn0039111 | Plip | DRSC14235 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 42807 | CG10371 | Plip | 104774 | np | none | | | | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00326-A | 42807 | FBgn0039111 | Plip | 104774 | np | none | | | | Synapse formation and maintenance | A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance | Valakh et al. | 2012 | 22542760 | Tissue | Elav-Gal4 | Neuromuscular junction morphology | Fluorescence | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | For further phenotype explanations sp. | GR00335-A | 42807 | FBgn0039111 | Plip | HMS02161 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 42807 | FBgn0039111 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 42807 | CG10371 | | 47623 | np | liquid clearance defect | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 42807 | CG10371 | | 104774 | -0.16939793628141198 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 42807 | CG10371 | | GD30698 | | none | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |