RNAi

Gene Info

  • Species:Fly (Drosophila melanogaster)
  • GeneID:43275
  • Symbol:Ser
  • Description:Serrate
DataSource: http://genomernai.dkfz.de/v16/genedetails/43275

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Stable_ID Entrez_ID Gene_ID Gene_Symbol Reagent_ID Score Phenotype Conditions Follow_Up Comment Screen_Title Publication_Title Authors Publication_Year Pubmed_ID Biosource Biomodel Assay Method Library Scope Reagent_Type Score_Type Cutoff Notes
GR00002-A 43275 FBgn0004197 Ser DRSC16856 -0.117191893 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00002-A 43275 FBgn0004197 Ser DRSC16856 0.289431137 none no Lipid storage COPI complex is a regulator of lipid homeostasis. Beller et al. 2008 19067489 Cell line Kc167 Nuclear to lipid droplet cross-sectional area Fluorescence np Genome-wide dsRNA B-score > 2 OR < -1.7 Additional information about a secondary screen with non-overlaping dsRNAs
GR00007-A-0 43275 FBgn0004197 DRSC16856 np Defasciculation no Neural outgrowth Identification of neural outgrowth genes using genome-wide RNAi. Sepp et al. 2008 18604272 Cell line Primary neural cells Morphology High content (microscopy) DRSC dsRNA Visual inspection np
GR00031-A-1 43275 HFA16856 0.3 none yes Cell growth and viability (1) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line Kc167 Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
GR00031-A-2 43275 HFA16856 -0.2 none no Cell growth and viability (2) Genome-wide RNAi analysis of growth and viability in Drosophila cells. Boutros et al. 2004 14764878 Cell line S2R+ Cell number and viability Luminescence Custom-made (HFA) Genome-wide dsRNA Z-score >
43275 FBgn0004197 Ser RE42104 sp none no library: DGC2
GR00128-A-2 43275 FBgn0004197 CG6127 DRSC25494 sp none no Combinatorial effect with methyl methanesulphonate (2) A network of conserved damage survival pathways revealed by a genomic RNAi screen. Ravi et al. 2009 19543366 Cell line Kc167 Viability (synthetic lethal) Luminescence Version 1 Selected genes dsRNA rp rp
GR00134-A-1 43275 CG6127 Ser 27172 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00134-A-1 43275 CG6127 Ser 3721 np none no Muscle morphogenesis and function (1) Systematic genetic analysis of muscle morphogenesis and function in Drosophila. Schnorrer et al. 2010 20220848 Tissue Mef2-GAL4 Posture, locomotion, flight and viability Visual inspection np Genome-wide UAS-IR construct rp S19 > 0.5
GR00135-A-1 43275 CG6127 Ser 27172 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 43275 CG6127 Ser 27174 -1.17 none yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00135-A-1 43275 CG6127 Ser 3721 0 Developmentally lethal yes Heat nociception (1) A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. Neely et al. 2010 21074052 Organism elav-GAL4 Noxious heat avoidance and viability Fly count np Genome-wide UAS-IR construct Z-score > 1.65 Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity)
GR00138-A-1 43275 CG6127 27172 1 none yes Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00138-A-1 43275 CG6127 3721 0.75 none yes Heart development and function (1) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Developmental lethality <
GR00138-A-2 43275 CG6127 27172 2.67 none no Heart development and function (2) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Z-score > 3
GR00138-A-2 43275 CG6127 3721 3.63 Decreased viability no Heart development and function (2) A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. Neely et al. 2010 20371351 Tissue TinCΔ4 12a-Gal4 Viability Fly count np Selected genes UAS-IR construct Z-score > 3
GR00139-A 43275 Ser 3721 sp Semi-lethal no Fly viability and adult morphology A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Dietzl et al. 2007 17625558 Organism Act5C-GAL4 Adult morphology and viability Visual inspection Custom-made Selected and random genes UAS-IR construct Phenotype strength np Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00139-A 43275 Ser 27172 sp Lethal no Fly viability and adult morphology A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Dietzl et al. 2007 17625558 Organism Act5C-GAL4 Adult morphology and viability Visual inspection Custom-made Selected and random genes UAS-IR construct Phenotype strength np Additional information about secondary screens (MS1096-GAL4, ey-GAL4, GMR-GAL4 and pnr-GAL4)
GR00142-A-1 43275 CG6127 Ser 27174 -0.688524590163934 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00142-A-1 43275 CG6127 Ser 27174 0.170454545454545 none no Serratia marcescens infection (1) Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. Cronin et al. 2009 19520911 Organism HSP70-GAL4; TubGAL80ts Heat shock and viability Fly count np Random genes UAS-IR construct Days life time (LT50) < -1.5 SD OR > 2 SD
GR00144-A-1 43275 CG6127 Ser 2C7 no phenotype none temperature: 18 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 43275 CG6127 Ser 2C7 no phenotype none temperature: 25 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-1 43275 CG6127 Ser 2C7 lethal Lethal temperature: 29 yes Notch pathway regulation (1) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue sca-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 43275 CG6127 Ser 2C7 lethal Adults die in food, escapers have no phenotype temperature: 25 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 43275 CG6127 Ser 2C7 lethal Adults die in food, pupal lethal temperature: 29 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-2 43275 CG6127 Ser 2C7 no phenotype none temperature: 18 yes Notch pathway regulation (2) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue fz3-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 43275 CG6127 Ser 2C7 no phenotype none temperature: 18 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 43275 CG6127 Ser 2C7 no phenotype none temperature: 25 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-3 43275 CG6127 Ser 2C7 no phenotype none temperature: 29 yes Notch pathway regulation (3) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Selected genes UAS-IR construct Phenotype strength np
GR00144-A-4 43275 CG6127 27172 3 Colour defects no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 43275 CG6127 27174 sp Colour defects, bristle morphology defects no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00144-A-4 43275 CG6127 3721 0 none no not lethal Notch pathway regulation (4) Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. Mummery-Widmer et al. 2009 19363474 Tissue pnr-GAL4 External sensory organ morphology and viability Visual inspection np Genome-wide UAS-IR construct Phenotype strength np
GR00189-A-1 43275 FBgn0004197 Ser BKN28416 np Downregulation of Notch pathway yes Notch pathway regulation (1) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Cell line S2 Notch pathway reporter Luminescence BKN Genome-wide dsRNA Complex, sp Complex criteria
GR00189-A-2 43275 FBgn0004197 Ser 27172 sp Downregulation of Notch pathway; wing disc NRE:EGFP: down yes en-GAL4 Notch pathway regulation (2) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue en-GAL4, C96-GAL4 and vg-GAL4 Notch pathway reporter, wing imaginal discs morphology, adult wing morphology and viability Fluorescence and visual inspection Notch pathway component enriched RNAi library Selected genes UAS-IR construct Phenotype strength np
GR00189-A-3 43275 FBgn0004197 Ser np np none no Notch pathway regulation (3) A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Saj et al. 2010 20493818 Tissue GMR-GAL4 Notch pathway reporter Fluorescence Notch pathway component enriched RNAi library Selected genes UAS-IR construct Average fluorescent intensity Complex criteria
GR00190-A-1 43275 FBgn0004197 Ser 27174 0.696 Decreased triglyceride expression yes Adiposity regulation (1) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Organism Hsp70-GAL4;Tub-GAL80ts Total fly triglyceride expression Colorimetrics np Genome-wide UAS-IR construct Triglyceride change Z-score > 1.65 after 3 screening rounds Additional information about the primary screen
GR00190-A-2 43275 FBgn0004197 Ser 27174 -0.079 none no Adiposity regulation (2) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue nsyb-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-3 43275 FBgn0004197 Ser 27174 -0.404 Decreased triglyceride expression no Adiposity regulation (3) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue C57-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-4 43275 FBgn0004197 Ser 27174 -0.557 Decreased triglyceride expression no Adiposity regulation (4) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue oe-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00190-A-5 43275 FBgn0004197 Ser 27174 0.127 none no Adiposity regulation (5) Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Pospisilik et al. 2010 20074523 Tissue ppl-GAL4 Total fly triglyceride expression Colorimetrics np Selected genes UAS-IR construct Triglyceride change > 0.25 OR <-0.25 (change >25%) Additional information about the primary screen
GR00218-S 43275 CG6127 Ser AMB33370 -1.35 none no Hippo pathway regulation Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Wehr et al. 2013 23263283 Cell line S2R+ Hippo pathway reporter Luminescence np Genome-wide dsRNA Z-score > 3 OR < -3 Author-submitted data. Primary screen
GR00284-A 43275 CG6127 Ser 108348 -0.3931210524796882 none piRNA pathway regulation A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway Czech et al. 2013 23665227 Tissue nos-GAL4 Transposon expression qPCR VDRC Selected genes UAS-IR construct Z-score Weak < -1.5; strong: < -2 KK and GD libraries used (see phenotype data)
GR00311-A 43275 FBgn0004197 Ser DRSC16856 np none Actin and microtubule morphology Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype Rohn et al. 2011 21893601 Cell line S2R+ Alpha-tubulin and F-actin protein expression Fluorescence DRSC Genome-wide dsRNA Visual inspection np Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2.
GR00315-A-1 43275 CG6127 Ser 108348 np none Follicular epithelium development (1) A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche Berns et al. 2014 24762813 Tissue Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 Follicular epithelium morphology Fluorescence VDRC KK Random genes UAS-IR construct Visual inspection np Further classification screen available, see follicular epithelium development (2).
GR00335-A 43275 FBgn0004197 Ser HMS01179 np Embryonic lethal many embryonic lethal Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
GR00335-A 43275 FBgn0004197 Ser JF03140 np none Germline stem cell (GSC) regulation A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal Yan et al. 2014 24576427 Tissue MTD-GAL4 or UAS-dcr2; nanos-GAL4 Spectrin and vasa protein expression Fluorescence TRiP Selected genes UAS-IR construct Visual inspection np More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used.
43275 FBgn0004197 np np sp none
GR00367-S 43275 CG6127 27172 np none VDRC Drosophila airway maturation Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes Hosono et al. 2015 26551273 Tissue btl-GAL4 Liquid clearance defect and viability Visual inspection VDRC, Trip, NIG Genome-wide UAS-IR construct Frequency >30%
GR00379-A-1 43275 CG6127 108348 -0.04928085500753396 none No phenotype in egg laying/hatching or larvae in egg laying or larvae Stem cell maintenance Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation Sanchez et al. 2016 26669894 Organism nanos-GAL4 nos and yTub37c mRNA expression qPCR VDRC Selected genes UAS-IR construct Z-score Low: 1 - 2.5; Medium: 2.5 - 4; High: > Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field.
GR00385-A 43275 108348 0.2373535791218718 Low performer in olfactory memory formation Regulation of olfactory memory formation Identification of genes that promote or inhibit olfactory memory formation in Drosophila. Walkinshaw et al. 2015 25644700 Organism Nsyb-GAL4 T-maze performance Visual inspection VDRC Selected genes UAS-IR construct Performance index, sp High performer in olfactory memory formation: > Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field.
GR00388-A 43275 CG6127 BL28713 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 43275 CG6127 GD27174 Lethal Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.
GR00388-A 43275 CG6127 BL34700 none Regulation of intestinal stem cells (ISC) Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila Zeng et al. 2015 25704823 Tissue act5C-GAL4 Viability Visual inspection VDRC and BDSC Genome-wide UAS-IR construct np np Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section.