| GR00002-A | 44432 | FBgn0024222 | ird5 | DRSC16714 | -0.07505239 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 44432 | FBgn0024222 | ird5 | DRSC16714 | 3.760418376 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00030-A-1 | 44432 | CG4201 | ird5 | ird5 | np | none | | yes | | Cell cycle regulation (1) | Genome-wide survey of protein kinases required for cell cycle progression. | Bettencourt-Dias et al. | 2004 | 15616552 | Cell line | S2 | alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content | Flow cytometry | rp | Kinases | dsRNA | Confidence intervals | Complex criteria | | GR00030-A-2 | 44432 | CG4201 | ird5 | ird5 | np | none | | no | | Cell cycle regulation (2) | Genome-wide survey of protein kinases required for cell cycle progression. | Bettencourt-Dias et al. | 2004 | 15616552 | Cell line | S2 | Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression | Flow cytometry | rp | Kinases and kinase regulators | dsRNA | np | np | | GR00031-A-1 | 44432 | | | HFA16714 | -0.1 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 44432 | | | HFA16714 | -0.3 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00041-A | 44432 | CG4201 | IKK | HFA16714 | -6.5 | Decreased relish reporter expression | | | | Immune deficiency (IMD) pathway regulation | An RNA interference screen identifies Inhibitor of Apoptosis Protein 2 as a regulator of innate immune signalling in Drosophila. | Gesellchen et al. | 2005 | 16170305 | Cell line | SL2 | Relish reporter (mtk) | Luminescence | HFA | Genome-wide | dsRNA | Z-score | < -2.4 OR > 2.4 | For validation screen with different reporter construct sp. Own data - check nones (ES: did not find any information) | | 44432 | FBgn0024222 | ird5 | CT13812 | sp | none | | no | library: PHOSPHO | | | | | | | | | | | | | | | | | 44432 | FBgn0024222 | ird5 | LD21354 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | GR00134-A-1 | 44432 | CG4201 | ird5 | 26427 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 44432 | CG4201 | ird5 | 26427 | 0.12 | none | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 44432 | CG4201 | | 26427 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 44432 | CG4201 | ird5 | 26427 | -0.683333333333333 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 44432 | CG4201 | | 26427 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00148-A | 44432 | FBgn0024222 | ird5 | np | 6.23 | Increased P-JNK protein expression with PGN | 15 min PGN induction | no | | PGN-induced dJNK phosphorylation | A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. | Bond and Foley | 2009 | 19893628 | Cell line | S2 | P-JNK protein expression | Fluorescence | Custom-made | Genome-wide | dsRNA | Z-score | Complex criteria | Only hits stored in GenomeRNAi | GR00148-A | 44432 | FBgn0024222 | ird5 | np | 7.74 | Increased P-JNK protein expression with PGN | 60 min PGN induction | no | | PGN-induced dJNK phosphorylation | A quantitative RNAi screen for JNK modifiers identifies Pvr as a novel regulator of Drosophila immune signaling. | Bond and Foley | 2009 | 19893628 | Cell line | S2 | P-JNK protein expression | Fluorescence | Custom-made | Genome-wide | dsRNA | Z-score | Complex criteria | Only hits stored in GenomeRNAi | GR00168-A | 44432 | | Ird5 | np | np | Decreased Drosomycin::luciferase protein expression after Toll10b and E. coli stimulation | | yes | | NF-kB pathway regulation | Genome-wide RNA interference in Drosophila cells identifies G protein-coupled receptor kinase 2 as a conserved regulator of NF-kappaB signaling. | Valanne et al. | 2010 | 20421637 | Cell line | S2 | Toll and Imd pathway Drosomycin reporter | Luminescence | Custom-made | Genome-wide | dsRNA | Reporter activity | < 50 % | | GR00190-A-1 | 44432 | FBgn0024222 | ird5 | 26427 | 0.08 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00201-A-1 | 44432 | FBgn0024222 | ird5 | ird5 (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (1) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | S2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-2 | 44432 | FBgn0024222 | ird5 | ird5 (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (2) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | S2R+ | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-3 | 44432 | FBgn0024222 | ird5 | ird5 (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (3) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | Kc167 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-4 | 44432 | FBgn0024222 | ird5 | ird5 (Baum_Lab) | np | Altered cell morphology | | yes | | Actin organization and cell morphology (4) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG2-c2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-5 | 44432 | FBgn0024222 | ird5 | ird5 (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (5) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG3-c1 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-6 | 44432 | FBgn0024222 | ird5 | ird5 (Baum_Lab) | np | Altered cell morphology | | no | | Actin organization and cell morphology (6) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG3-c2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00202-A | 44432 | FBgn0024222 | ird5 | np | sp | none | | no | | Vaccinia virus infection | A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. | Moser et al. | 2010 | 20585561 | Cell line | DL1 | Virally encoded beta-galactosidase protein expression | Fluorescence | Custom-made | Kinases, phosphatases and selected genes | dsRNA | Z-score | < -2 in both duplicates | Author-reviewed data | GR00218-S | 44432 | CG4201 | ird5 | AMB20842 | 1.5 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00282-A | 44432 | CG4201 | ird5 | 26427 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 44432 | CG4201 | ird5 | 26427 | 0.21011030848766094 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 44432 | FBgn0024222 | ird5 | DRSC16714 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00335-A | 44432 | FBgn0024222 | ird5 | GL00058 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 44432 | FBgn0024222 | np | np | sp | none | | | | | | | | | | | | | | | | | | | | 44432 | FBgn0024222 | np | np | -2.95 | Increased transposon expression | | | fold change: 7.54 | | | | | | | | | | | | | | | | GR00339-A-2 | 44432 | FBgn0024222 | ird5 | 26427 | 1 | none | | | | piRNA pathway regulation (2) | A Genome-wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary piRNA Biogenesis in Drosophila | Muerdter et al. | 2013 | 23665228 | Tissue | traffic jam GAL4 | Gypsy and ZAM transposon expression | qPCR | VDRC (GD, KK) and TRiP | Selected genes | UAS-IR construct | Fold change | > | Following transposons were used for scoring: Gypsy for KK and ZAM for GD RNAi lines. TRIP reagents als TRIP_11111 dargestellt, Suchbarkeit? Developmental defect: wurden nicht gemessen, passt np als Score? | GR00367-S | 44432 | CG4201 | | 26427 | np | lethal or adult morphology phenotype | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00370-A | 44432 | FBgn0024222 | ird5 | | 0.09 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00370-A | 44432 | FBgn0024222 | ird5 | | -1.11 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00370-A | 44432 | FBgn0024222 | ird5 | | -0.03 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00379-A-1 | 44432 | CG4201 | | 26427 | -0.39666749944104573 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 44432 | CG4201 | | GD26427 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |