GR00002-A | 49701 | FBgn0011253 | gish | DRSC16154 | 0.147621152 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 49701 | FBgn0011253 | gish | DRSC16154 | 4.439734272 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00030-A-1 | 49701 | CG6963 | gish | gish, gish_2 | sp | Increased S DNA content, aneuploid cells, increased G2/M DNA content, increased mitotic index, spindle defects | | yes | | Cell cycle regulation (1) | Genome-wide survey of protein kinases required for cell cycle progression. | Bettencourt-Dias et al. | 2004 | 15616552 | Cell line | S2 | alpha-tubulin and gamma-tubulin protein expression, histone H3 phosphorylation and DNA content | Flow cytometry | rp | Kinases | dsRNA | Confidence intervals | Complex criteria | | GR00030-A-2 | 49701 | CG6963 | gish | gish, gish_2 | np | Increased S DNA content or increased number of aneuploid cells, increased G2 DNA content | | no | weak phenotype | Cell cycle regulation (2) | Genome-wide survey of protein kinases required for cell cycle progression. | Bettencourt-Dias et al. | 2004 | 15616552 | Cell line | S2 | Histone H3 phosphorylation, Cyclin A and Cyclin B protein expression | Flow cytometry | rp | Kinases and kinase regulators | dsRNA | np | np | | GR00031-A-1 | 49701 | | | HFA16154 | 0.6 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 49701 | | | HFA16154 | 0.7 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 49701 | FBgn0011253 | gish | CT21414 | sp | none | | no | library: PHOSPHO | | | | | | | | | | | | | | | | | 49701 | FBgn0011253 | gish | CT39940 | sp | none | | no | library: PHOSPHO | | | | | | | | | | | | | | | | | 49701 | FBgn0011253 | gish | np | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 49701 | FBgn0011253 | gish | LD28216 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 49701 | FBgn0011253, FBgn0053188 | gish, CG33188 | np | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | | 49701 | FBgn0011253, FBgn0053188 | gish, CG33188 | SD09393 | sp | none | | no | library: DGC1 | | | | | | | | | | | | | | | | GR00089-A | 49701 | | CG6963 | np | 8.0E-4 | Decreased dFoxO reporter expression | | | | FoxO regulation | RNAi screening for kinases and phosphatases identifies FoxO regulators | Mattila et al. | 2008 | 18815370 | Cell line | S2 | dFoxO reporter expression | Fluorescence | Custom-made | Kinases and phosphatases | dsRNA | P-value | < | Genome RNAi mapping in column after comment (FBgn) | GR00134-A-1 | 49701 | CG6963 | gish | 26003 | np | none | | no | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 49701 | CG6963 | gish | 26003 | 3.5 | Increased noxious heat avoidance | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 49701 | CG6963 | | 26003 | 0.92 | none | | yes | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00138-A-2 | 49701 | CG6963 | | 26003 | 3.97 | Decreased viability | | no | | Heart development and function (2) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00142-A-1 | 49701 | CG6963 | CG6963 | 26003 | 0.10126582278481 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 49701 | CG6963 | | 26003 | sp | Loss of bristles, bristle morphology defects, notum malformation migration | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00190-A-1 | 49701 | FBgn0250823 | gish | 26003 | -0.178 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00191-A-1 | 49701 | CG6963 | gish | np | np | none | | yes | | Blood cell homeostasis regulation (1) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-2 | 49701 | CG6963 | gish | np | np | none | | no | | Blood cell homeostasis regulation (2) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00201-A-1 | 49701 | FBgn0011253 | gish | gish (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (1) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | S2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-2 | 49701 | FBgn0011253 | gish | gish (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (2) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | S2R+ | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-3 | 49701 | FBgn0011253 | gish | gish (Baum_Lab) | np | none | | yes | | Actin organization and cell morphology (3) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | Kc167 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-4 | 49701 | FBgn0011253 | gish | gish (Baum_Lab) | np | Altered cell morphology | | yes | | Actin organization and cell morphology (4) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG2-c2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-5 | 49701 | FBgn0011253 | gish | gish (Baum_Lab) | np | Altered cell morphology | | yes | | Actin organization and cell morphology (5) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG3-c1 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00201-A-6 | 49701 | FBgn0011253 | gish | gish (Baum_Lab) | np | Altered cell morphology | | no | | Actin organization and cell morphology (6) | Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Liu et al. | 2009 | 19265526 | Cell line | BG3-c2 | alpha-Tubulin and F-actin protein expression | Fluorescence | Custom-made | Kinases | dsRNA | Visual inspection | np | | GR00202-A | 49701 | FBgn0011253 | gish | np | sp | none | | no | | Vaccinia virus infection | A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. | Moser et al. | 2010 | 20585561 | Cell line | DL1 | Virally encoded beta-galactosidase protein expression | Fluorescence | Custom-made | Kinases, phosphatases and selected genes | dsRNA | Z-score | < -2 in both duplicates | Author-reviewed data | GR00214-A-1 | 49701 | FBgn0250823 | gish | DRSC16154 | 1.35 | none | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 49701 | FBgn0250823 | gish | DRSC16154 | 0.77 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 49701 | FBgn0250823 | gish | DRSC16154 | 0.28 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 49701 | FBgn0250823 | gish | DRSC16154 | 7.74 | Increased ERK phosphorylation after sSpitz and EGF stimulation | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00216-A | 49701 | FBgn0250823 | gish | BKN28680 | -0.3 | none | | no | | Wnt proteins secretion | p24 proteins are required for secretion of Wnt ligands. | Buechling et al. | 2011 | 22094269 | Cell line | S2R+ | Wg pathway reporter | Luminescence | BKN | Cellular transporting machinery genes | dsRNA | Z-score | > 2.5 OR < -2.5 | Author-reviewed data. Cutoff criteria. Additional information about secondary in vivo screens (act-GAL4, vg-GAL4, nub-GAL4 and wg-GAL4) | GR00218-S | 49701 | CG6963 | gish | AMB21891 | 1.06 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 49701 | CG6963 | | 6963R | sp | none | | no | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00282-A | 49701 | CG6963 | gish | 106826 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 49701 | CG6963 | gish | 26003 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 49701 | CG6963 | gish | 106826 | 0.8347294216715153 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00311-A | 49701 | FBgn0250823 | gish | DRSC16154 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 49701 | CG6963 | gish | 106826 | np | Effect on follicular epithelium morphology | | | further phenotype data in classification screen | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 49701 | CG6963 | gish | 106826 | np | Defect in formation phase | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | GR00326-A | 49701 | FBgn0250823 | gish | 106826 | np | none | | | | Synapse formation and maintenance | A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance | Valakh et al. | 2012 | 22542760 | Tissue | Elav-Gal4 | Neuromuscular junction morphology | Fluorescence | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | For further phenotype explanations sp. | GR00335-A | 49701 | FBgn0250823 | gish | JF02903 | np | none | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 49701 | FBgn0250823 | gish | GL00005 | np | Embryonic lethal | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | GR00335-A | 49701 | FBgn0250823 | gish | HMS01609 | np | Embryonic lethal | | | many embryonic lethal | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 49701 | FBgn0250823 | np | np | sp | none | | | | | | | | | | | | | | | | | | | GR00367-S | 49701 | CG6963 | | 106826 | np | none | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00370-A | 49701 | FBgn0250823 | gish | | -0.15 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00370-A | 49701 | FBgn0250823 | gish | | 0.68 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00370-A | 49701 | FBgn0250823 | gish | | 0.6 | none | | | | Regulation of Nrf2 homolog CncC | Cdk12 Is A Gene-Selective RNA Polymerase II Kinase That Regulates a Subset of the Transcriptome, Including Nrf2 Target Genes | Li et al. | 2016 | 26911346 | Cell line | S2 | CncC reporter | Luminescence | HD2 | Kinases | dsRNA | Z-Score | <-1.65 OR >1.65 | | GR00379-A-1 | 49701 | CG6963 | | 106826 | -0.18962673934059782 | none | | | No phenotype in egg laying/hatching or larvae in egg laying or larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00388-A | 49701 | CG6963 | | GD26003 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 49701 | CG6963 | | BL28066 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |