| GR00002-A | 53428 | FBgn0040066 | wds | DRSC18852 | -0.048267546 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00002-A | 53428 | FBgn0040066 | wds | DRSC18852 | -0.703275661 | none | | no | | Lipid storage | COPI complex is a regulator of lipid homeostasis. | Beller et al. | 2008 | 19067489 | Cell line | Kc167 | Nuclear to lipid droplet cross-sectional area | Fluorescence | np | Genome-wide | dsRNA | B-score | > 2 OR < -1.7 | Additional information about a secondary screen with non-overlaping dsRNAs | GR00004-A-0 | 53428 | | wds | DRSC18852 | 1.39 | siRNA reporter upregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00004-A-0 | 53428 | | wds | DRSC18852 | 1.62 | miRNA reporter upregulated | | no | | Drosophila small RNA pathways | Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. | Zhou et al. | 2008 | 19026789 | Cell line | S2 | miRNA and siRNA pathway activity | Dual luciferase | DRSC | | dsRNA | Fold change endo-siRNA reporter | 1.5/0.6 | | GR00031-A-1 | 53428 | | | HFA18852 | -0.1 | none | | yes | | Cell growth and viability (1) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | Kc167 | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | GR00031-A-2 | 53428 | | | HFA18852 | -0.3 | none | | no | | Cell growth and viability (2) | Genome-wide RNAi analysis of growth and viability in Drosophila cells. | Boutros et al. | 2004 | 14764878 | Cell line | S2R+ | Cell number and viability | Luminescence | Custom-made (HFA) | Genome-wide | dsRNA | Z-score | > | | | 53428 | FBgn0040066 | wds | RE31658 | sp | Decreased G1 DNA content | | yes | library: DGC2 | | | | | | | | | | | | | | | | | 53428 | FBgn0040066 | wds | LD10938 | sp | none | | yes | library: DGC2 | | | | | | | | | | | | | | | | GR00048-A-2 | 53428 | FBgn0040066 | wds | np | 1.203 | none | | yes | | Cell size and cell-cycle regulation (2) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Proliferation and viability | Colorimetrics | | Selected genes | dsRNA | MTS metabolic activity | > 2.5 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00048-A-3 | 53428 | FBgn0040066 | wds | np | 455 | none | | no | | Cell size and cell-cycle regulation (3) | Identification of pathways regulating cell size and cell-cycle progression by RNAi. | Bjӧrklund et al. | 2006 | 16496002 | Cell line | S2 | Cell number | Fluorescence | | Selected genes | dsRNA | Average cell number per field | > 2 standard deviations | Additional information about the primary screen (pooled library) and a secondary screen (number of binucleate cells) | GR00062-A | 53428 | FBgn0040066 | wds | np | 4 | Decreased Ca2+ influx | | | | Ca2+ channel regulation | CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. | Vig et al. | 2006 | 16645049 | Cell line | S2R+ | Ca2+ influx | Fluorescence | np | Genome-wide | dsRNA | Complex, sp | > | | GR00134-A-1 | 53428 | CG17437 | wds | 38926 | np | Lethal | | yes | early pupal | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-1 | 53428 | CG17437 | wds | 38925 | np | none | | yes | | Muscle morphogenesis and function (1) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | np | Genome-wide | UAS-IR construct | rp | S19 > 0.5 | | GR00134-A-2 | 53428 | CG17437 | wds | np | np | Wing posture / locomotion / ability to fly defect or lethal / semi-lethal | | no | | Muscle morphogenesis and function (2) | Systematic genetic analysis of muscle morphogenesis and function in Drosophila. | Schnorrer et al. | 2010 | 20220848 | Tissue | Mef2-GAL4 | Posture, locomotion, flight and viability | Visual inspection | Custom-made | Selected genes | UAS-IR construct | rp | S19 > 0.5 | | GR00135-A-1 | 53428 | CG17437 | wds | 38926 | -1.17 | Developmentally lethal | | yes | | Heat nociception (1) | A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene. | Neely et al. | 2010 | 21074052 | Organism | elav-GAL4 | Noxious heat avoidance and viability | Fly count | np | Genome-wide | UAS-IR construct | Z-score | > 1.65 | Additional information about secondary screens (geotactic, phototaxis, and temperature sensitivity) | GR00138-A-1 | 53428 | CG17437 | | 38925 | 0.75 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00138-A-1 | 53428 | CG17437 | | 38926 | 1 | none | | no | | Heart development and function (1) | A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function. | Neely et al. | 2010 | 20371351 | Tissue | TinCΔ4 12a-Gal4 | Viability | Fly count | np | Selected genes | UAS-IR construct | Developmental lethality | < | | GR00142-A-1 | 53428 | CG17437 | wds | 38926 | 0.519480519480519 | none | | no | | Serratia marcescens infection (1) | Genome-wide RNAi screen identifies genes involved in intestinal pathogenic bacterial infection. | Cronin et al. | 2009 | 19520911 | Organism | HSP70-GAL4; TubGAL80ts | Heat shock and viability | Fly count | np | Random genes | UAS-IR construct | Days life time (LT50) | < -1.5 SD OR > 2 SD | | GR00144-A-4 | 53428 | CG17437 | | 38925 | 0 | none | | no | not lethal | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00144-A-4 | 53428 | CG17437 | | 38926 | sp | Completely lethal (pupal) | | no | | Notch pathway regulation (4) | Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi. | Mummery-Widmer et al. | 2009 | 19363474 | Tissue | pnr-GAL4 | External sensory organ morphology and viability | Visual inspection | np | Genome-wide | UAS-IR construct | Phenotype strength | np | | GR00172-A-1 | 53428 | CG17437, CG6754 | wds, nbs | DRSC18852, custom (nbs) | np | Increased histone H3 phosphorylation with doxorubicin | | yes | | G2-M DNA damage checkpoint regulation (1) | A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint. | Kondo and Perrimon | 2011 | 21205937 | Cell line | S2R+ | Histone H3 phosphorylation | Fluorescence | DRSC 2.0 and custom-made | Genome-wide | dsRNA | Mitotic cell number per well | > 20 | | GR00172-A-2 | 53428 | CG17437 | wds | DRSC18852 | 18.5 | Increased histone H3 phosphorylation with bleocin | | yes | with bleocin | G2-M DNA damage checkpoint regulation (2) | A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint. | Kondo and Perrimon | 2011 | 21205937 | Cell line | S2R+ | Histone H3 phosphorylation | Fluorescence | DRSC 2.0 and custom-made | Selected genes | dsRNA | Relative mitotic index | Complex criteria | Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field. | GR00172-A-2 | 53428 | CG17437 | wds | DRSC18852 | 43.58 | Increased histone H3 phosphorylation with doxorubicin | | yes | with doxorubicin | G2-M DNA damage checkpoint regulation (2) | A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint. | Kondo and Perrimon | 2011 | 21205937 | Cell line | S2R+ | Histone H3 phosphorylation | Fluorescence | DRSC 2.0 and custom-made | Selected genes | dsRNA | Relative mitotic index | Complex criteria | Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field. | GR00172-A-2 | 53428 | CG17437 | wds | DRSC18852 | 33.1 | Increased histone H3 phosphorylation with etoposide | | yes | with etoposide | G2-M DNA damage checkpoint regulation (2) | A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint. | Kondo and Perrimon | 2011 | 21205937 | Cell line | S2R+ | Histone H3 phosphorylation | Fluorescence | DRSC 2.0 and custom-made | Selected genes | dsRNA | Relative mitotic index | Complex criteria | Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field. | GR00172-A-2 | 53428 | CG17437 | wds | DRSC18852 | 46.64 | Increased histone H3 phosphorylation after X-ray exposure | | yes | X-ray exposure | G2-M DNA damage checkpoint regulation (2) | A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint. | Kondo and Perrimon | 2011 | 21205937 | Cell line | S2R+ | Histone H3 phosphorylation | Fluorescence | DRSC 2.0 and custom-made | Selected genes | dsRNA | Relative mitotic index | Complex criteria | Several genes were considered potential false positives because they were likely to increase the mitotic index by delaying mitotic exit irrespective of the DNA damage checkpoint - see comment field. | GR00183-A | 53428 | FBgn0040066 | wds | 38926 | sp | GFP aggregates number, GFP aggregates size, ganglion mother cell shorter lineages, neuroblast underproliferation, lethal | | no | | Self-renewal and differentiation in neural stem cells | Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. | Neumueller et al. | 2011 | 21549331 | Tissue | insc-GAL4 | Number and size of neuroblasts, ganglion mother cells, intracellular GFP aggregates and viability | Fluorescence | np | Genome-wide | UAS-IR construct | Phenotype strength | > 1 | Additional information about a secondary screen (KK library) | GR00190-A-1 | 53428 | FBgn0040066 | wds | 38926 | -0.574 | none | | no | | Adiposity regulation (1) | Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. | Pospisilik et al. | 2010 | 20074523 | Organism | Hsp70-GAL4;Tub-GAL80ts | Total fly triglyceride expression | Colorimetrics | np | Genome-wide | UAS-IR construct | Triglyceride change | Z-score > 1.65 after 3 screening rounds | Additional information about the primary screen | GR00191-A-1 | 53428 | CG17437 | wds | np | np | Increased melanotic mass formation | | yes | | Blood cell homeostasis regulation (1) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-2 | 53428 | CG17437 | wds | np | np | none | | yes | | Blood cell homeostasis regulation (2) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | np | np | | GR00191-A-3 | 53428 | CG17437 | wds | np | 0 | none | | yes | | Blood cell homeostasis regulation (3) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00191-A-4 | 53428 | CG17437 | wds | np | 0 | none | | yes | | Blood cell homeostasis regulation (4) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00191-A-5 | 53428 | CG17437 | wds | np | 52.4 | Increased melanotic mass formation | | yes | | Blood cell homeostasis regulation (5) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | cg-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00191-A-6 | 53428 | CG17437 | wds | np | 4 | none | | yes | confirmed melanotic suppressor gene, non-overlapping dsRNA | Blood cell homeostasis regulation (6) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | srp-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00191-A-7 | 53428 | CG17437 | wds | np | 0 | none | | yes | confirmed melanotic suppressor gene, non-overlapping dsRNA | Blood cell homeostasis regulation (7) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | hml∆-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00191-A-8 | 53428 | CG17437 | wds | np | 43.4 | Increased melanotic mass formation | | no | confirmed melanotic suppressor gene, non-overlapping dsRNA | Blood cell homeostasis regulation (8) | An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. | Avet-Rochex et al. | 2010 | 20540764 | Tissue | cg-GAL4 | Melanotic mass formation | Microscopy | np | Selected genes | UAS-IR construct | Tumor index | > | | GR00214-A-1 | 53428 | FBgn0040066 | wds | DRSC18852 | 0.01 | none | 0 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-1 | 53428 | FBgn0040066 | wds | DRSC18852 | -3.01 | Decreased ERK phosphorylation after EGF stimulation | 10 minutes EGF stimulation | yes | | RTK-Ras-ERK pathway regulation (1) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | S2R+ | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 53428 | FBgn0040066 | wds | DRSC18852 | -0.44 | none | 10 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00214-A-2 | 53428 | FBgn0040066 | wds | DRSC18852 | 0.14 | none | 30 minutes sSpitz stimulation | no | | RTK-Ras-ERK pathway regulation (2) | Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. | Friedman et al. | 2011 | 22028469 | Cell line | Kc167 | ERK phosphorylation | Fluorescence | np | Genome-wide | dsRNA | Z-score | > 1.5 OR < -1.5 | | GR00218-S | 53428 | CG17437 | | AMB20177 | 1.2 | none | | no | | Hippo pathway regulation | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila | Wehr et al. | 2013 | 23263283 | Cell line | S2R+ | Hippo pathway reporter | Luminescence | np | Genome-wide | dsRNA | Z-score | > 3 OR < -3 | Author-submitted data. Primary screen | GR00239-A-1 | 53428 | CG17437 | | 17437R | sp | none | | no | | Glycosylation regulation (1) | Identification of genes required for neural-specific glycosylation using functional genomics. | Yamamoto-Hino et al. | 2010 | 21203496 | Tissue | GMR-GAL4 | Chaoptin (Chp) protein expression, alpha1,3-fucose, alpha1,6-fucose, (GlcNAc)n, GlcNAcb1-4GlcNAc and mannose Chp glycosylation and viability | Luminescence | np | Selected genes | UAS-IR construct | Z-score | > 3 | | GR00282-A | 53428 | CG17437 | wds | 38925 | np | none | | | | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00282-A | 53428 | CG17437 | wds | 38926 | np | none | | | rudimentary ovaries | piRNA pathway regulation | The Genetic Makeup of the Drosophila piRNA Pathway | Handler | 2013 | 23665231 | Tissue | tj-GAL4 | Gypsy transposon expression | Microscopy | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | See comment for ovarian morphology data. Gene expression data shown in the comment derive from an ovarian stem cell line. VDRC library: KK and GD used, see phenotype data | GR00284-A | 53428 | CG17437 | wds | 105371 | -0.7305743215215261 | none | | | | piRNA pathway regulation | A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway | Czech et al. | 2013 | 23665227 | Tissue | nos-GAL4 | Transposon expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Weak < -1.5; strong: < -2 | KK and GD libraries used (see phenotype data) | GR00308-A | 53428 | CG17437 | | np | 2.33 | Decreased cell aggregation | | | | Cadherin-mediated cell-cell adhesion | A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion | Toret et al. | 2014 | 24446484 | Cell line | S2 | Cell aggregation | Fluorescence | V2 RNAi library (Thermo Fisher Scientific) | Genome-wide | dsRNA | Visual inspection; average of 3 replicates; 0 | > 1.5 | Additional secondary screen available. S2 cells stably express DE-cadherin. | GR00311-A | 53428 | FBgn0040066 | wds | DRSC18852 | np | none | | | | Actin and microtubule morphology | Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype | Rohn et al. | 2011 | 21893601 | Cell line | S2R+ | Alpha-tubulin and F-actin protein expression | Fluorescence | DRSC | Genome-wide | dsRNA | Visual inspection | np | Exact phenotypes are shown in the comment section. Several genes were indicated as hits but no phenotype was provided. The screen data plus images is available at http://jcb-dataviewer.rupress.org/jcb/browse/4609/S2. | GR00315-A-1 | 53428 | CG17437 | wds | 105371 | np | Effect on follicular epithelium morphology | | | further phenotype data in classification screen | Follicular epithelium development (1) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | Escargot-GAL4 and GR1-GAL4, GR1-GAL4, traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Random genes | UAS-IR construct | Visual inspection | np | Further classification screen available, see follicular epithelium development (2). | GR00315-A-2 | 53428 | CG17437 | wds | 105371 | np | No ovaries | | | | Follicular epithelium development (2) | A genome-scale in vivo RNAi analysis of epithelial development in Drosophila identifies new proliferation domains outside of the stem cell niche | Berns et al. | 2014 | 24762813 | Tissue | traffic jam-GAL4 | Follicular epithelium morphology | Fluorescence | VDRC KK | Selected genes | UAS-IR construct | Visual inspection | np | For more detailed phenotypes sp. Cell morphology was analized by aPKC and DLG protein expression. Additional validation screens available. | GR00326-A | 53428 | FBgn0040066 | wds | 17437 | np | none | | | | Synapse formation and maintenance | A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance | Valakh et al. | 2012 | 22542760 | Tissue | Elav-Gal4 | Neuromuscular junction morphology | Fluorescence | VDRC | Selected genes | UAS-IR construct | Visual inspection | np | For further phenotype explanations sp. | GR00335-A | 53428 | FBgn0040066 | wds | HMS00746 | np | Agametic | | | | Germline stem cell (GSC) regulation | A Regulatory Network of Drosophila Germline Stem Cell Self-Renewal | Yan et al. | 2014 | 24576427 | Tissue | MTD-GAL4 or UAS-dcr2; nanos-GAL4 | Spectrin and vasa protein expression | Fluorescence | TRiP | Selected genes | UAS-IR construct | Visual inspection | np | More detailed phenotypes for some genes are shown in the comment. Various VALIUM vectors were used. | | 53428 | FBgn0040066 | np | np | sp | none | | | | | | | | | | | | | | | | | | | | 53428 | FBgn0040066 | np | np | -2.03 | Increased transposon expression | | | fold change: 3.53 | | | | | | | | | | | | | | | | GR00339-A-2 | 53428 | FBgn0040066 | wds | 105371 | na | Developmental defect | | | | piRNA pathway regulation (2) | A Genome-wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary piRNA Biogenesis in Drosophila | Muerdter et al. | 2013 | 23665228 | Tissue | traffic jam GAL4 | Gypsy and ZAM transposon expression | qPCR | VDRC (GD, KK) and TRiP | Selected genes | UAS-IR construct | Fold change | > | Following transposons were used for scoring: Gypsy for KK and ZAM for GD RNAi lines. TRIP reagents als TRIP_11111 dargestellt, Suchbarkeit? Developmental defect: wurden nicht gemessen, passt np als Score? | GR00339-A-2 | 53428 | FBgn0040066 | wds | 38925 | 1 | none | | | | piRNA pathway regulation (2) | A Genome-wide RNAi Screen Draws a Genetic Framework for Transposon Control and Primary piRNA Biogenesis in Drosophila | Muerdter et al. | 2013 | 23665228 | Tissue | traffic jam GAL4 | Gypsy and ZAM transposon expression | qPCR | VDRC (GD, KK) and TRiP | Selected genes | UAS-IR construct | Fold change | > | Following transposons were used for scoring: Gypsy for KK and ZAM for GD RNAi lines. TRIP reagents als TRIP_11111 dargestellt, Suchbarkeit? Developmental defect: wurden nicht gemessen, passt np als Score? | GR00367-S | 53428 | CG17437 | | 38926 | np | liquid clearance defect | | | VDRC | Drosophila airway maturation | Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes | Hosono et al. | 2015 | 26551273 | Tissue | btl-GAL4 | Liquid clearance defect and viability | Visual inspection | VDRC, Trip, NIG | Genome-wide | UAS-IR construct | Frequency | >30% | | GR00379-A-1 | 53428 | CG17437 | | 105371 | 6.2204311106737045 | High decrease in nos and yTub37c expression | | | No eggs/larvae | Stem cell maintenance | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | nos and yTub37c mRNA expression | qPCR | VDRC | Selected genes | UAS-IR construct | Z-score | Low: 1 - 2.5; Medium: 2.5 - 4; High: > | Selected genes are: transcriptome-wide in female Drosophila. Additional phenotypes (egg laying/hatching) noted in the comment section. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00379-A-2 | 53428 | CG17437 | | 105371 | np | Germarium defects in 30%; No phenotype in 70% | | | | Defects in female germline | Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation | Sanchez et al. | 2016 | 26669894 | Organism | nanos-GAL4 | Vasa AND 1B1 expression | Immunofluorescence | VDRC | Selected genes | UAS-IR construct | np | np | Selected genes are: transcriptome-wide in female Drosophila. Female F1 scoring high in a previous screen were considered. Phenotypes were assesed visually in 864 candidates after dissection of >100 ovarioles for each candidate. Subset libraries from VDRC (KK or GD) can be found in a column next to the comment field. | GR00385-A | 53428 | | | 105371 | 0.18 | Low performer in olfactory memory formation | | | Final hit | Regulation of olfactory memory formation | Identification of genes that promote or inhibit olfactory memory formation in Drosophila. | Walkinshaw et al. | 2015 | 25644700 | Organism | Nsyb-GAL4 | T-maze performance | Visual inspection | VDRC | Selected genes | UAS-IR construct | Performance index, sp | High performer in olfactory memory formation: > | Genes were preselected for relevance to CNS development and function using bioinformatic criteria. Additionally, defects in wing formation are considered in the comments column. We could not confirm the exact number of primary hits; however, final hits are noted in the comments field. | GR00388-A | 53428 | CG17437 | | BL32952 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. | GR00388-A | 53428 | CG17437 | | GD38926 | | Lethal | | | | Regulation of intestinal stem cells (ISC) | Genome-wide RNAi Screen Identifies Networks Involved in Intestinal Stem Cell Regulation in Drosophila | Zeng et al. | 2015 | 25704823 | Tissue | act5C-GAL4 | Viability | Visual inspection | VDRC and BDSC | Genome-wide | UAS-IR construct | np | np | Lethal transgenic lines identified from the primary screen were crossed with temperature-sensitive esg-GAL4 flies. Phenotypes of ISCs from the dissected midguts are shown in the "Comment" section. |