| GR00053-A | 378807 | XM_371237 | CATSPER4 | np | sp | none | | no | | Genome stability | A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability. | Paulsen et al. | 2009 | 19647519 | Cell line | HeLa | gamma-H2AX phosphorylation and DNA content | Fluorescence | siARRAY human genome siRNA library | Genome-wide | siRNA | p-value | Complex criteria | Confidence groupings from 4 to 1 (highest level of confidence in group 4) | GR00054-A | 378807 | XM_371237 | CATSPER4 | np | 0.993 | none | | no | | Combinatorial effect with paclitaxel | Synthetic lethal screen identification of chemosensitizer loci in cancer cells. | Whitehurst et al. | 2007 | 17429401 | Cell line | NCI-H1155 | Viability (synthetic lethal) | ATP level | # G-005000-01 | Genome-wide | siRNA | Paclitaxel/control ratio | Complex criteria | Additional information about 87 high-confidence hits | GR00056-A | 378807 | XM_371237 | CATSPER4 | np | 1.114 | none | | no | | Melanogenesis | Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. | Ganesan et al. | 2008 | 19057677 | Cell line | MNT-1 | Melanin protein expression and viability | Absorbance and luminescence | rp | Genome-wide | siRNA | Normalized absorbance ratio | > 2 standard deviations below mean | Additional information about a secondary screen (retest to determine false-positive rate) | GR00057-A-1 | 378807 | XM_371237 | CATSPER4 | M-027514-00 | -0.31 | none | | no | | Wnt/beta-catenin pathway regulation (1) | A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. | Tang et al. | 2008 | 18621708 | Cell line | HeLa | Wnt pathway reporter | Luminescence | Human siArray siRNA library | Genome-wide | siRNA | Z-score | > 4 | Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). | GR00057-A-2 | 378807 | XM_371237 | CATSPER4 | M-027514-00 | sp | none | | no | | Wnt/beta-catenin pathway regulation (2) | A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. | Tang et al. | 2008 | 18621708 | Cell line | HeLa | Wnt pathway reporter | Luminescence | Human siArray siRNA library | Genome-wide | siRNA | Complex, SP | Complex criteria | Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). | GR00133-A-1 | 378807 | 378807 | CATSPER4 | np | 0.418695 | none | | no | | Salmonella enterica subspecies 1 serovar Typhimurium invasion (1) | RNAi screen of Salmonella invasion shows role of COPI in membrane targeting of cholesterol and Cdc42. | Misselwitz et al. | 2011 | 21407211 | Cell line | HeLa | Gentamycin protection invasion assay | Fluorescence | Druggable genome library V2.0 | Druggable genes | siRNA | log2 median | Complex criteria | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 256714 | 40.29 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 256714 | 59.92 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 256715 | 57.34 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 256715 | 25 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 256716 | -16.88 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 256716 | -43.13 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 290274 | 93 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 378807 | 378807 | CATSPER4 | 290274 | np | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00151-A-1 | 378807 | ENSG00000188782 | CATSPER4 | np | -0.782 | none | | no | | Homologous recombination DNA double-strand break repair (HR-DSBR) (1) | A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. | Słabicki et al. | 2010 | 20613862 | Cell line | HeLa | (HR-DSBR) DR-GFP reporter | Flow cytometry | Custom-made | Genome-wide | esiRNA | Z-score | < -2 OR > 2 | | GR00180-A-1 | 378807 | 378807 | CATSPER4 | PL-50080 | 0.92 | none | | no | | Hepatitis C virus replication (1) | A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. | Tai et al. | 2009 | 19286138 | Cell line | Huh7/Rep-Feo | HCV replicon RNA copy number | Luminescence | siARRAY Human Genome siRNA Library | Genome-wide | siRNA | q-value | Complex criteria | | GR00184-A-1 | 378807 | XM_371237 | CATSPER4 | M-027514-00 | -1.93641473719585 | none | | no | | Self-renewal and pluripotency in human embryonic stem cells (1) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | POU5F1 protein expression | Fluorescence | SMARTpool siRNA library | Genome-wide | siRNA | Z-score | < -2 | | GR00196-A-1 | 378807 | ENSG00000188782 | | np | sp | none | | no | | TP53 interactions (1) | A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. | Krastev et al. | 2011 | 21642980 | Cell line | HCT116 ( wildtype and TP53 knockout) | TP53 protein expression and viability | Fluorescence | rp | Genome-wide | esiRNA | Complex, sp | Complex criteria | | GR00197-A-1 | 378807 | 378807 | CATSPER4 | M-027514-00 | 1.130319299 | none | | no | | Human papillomavirus oncogene expression regulation (1) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Genome-wide | siRNA | Z-score | > | Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5 | GR00236-A-1 | 378807 | 378807 | CATSPER4 | M-027514-00 | 0.447872867976201 | none | | no | | Homologous recombination DNA double-strand break repair (HR-DSBR) (1) | A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. | Adamson et al. | 2012 | 22344029 | Cell line | DR-U2OS | (HR-DSBR) DR-GFP reporter and DNA content | Fluorescence | Human siGENOME siRNA (G-005000-05) | Genome-wide | siRNA | Relative HR ratio | < ~0.4 OR > 1.88 | Cutoff values correspond 2 standard deviations from the screen-wide mean | GR00240-S-1 | 378807 | XM_371237 | CATSPER4 | M-027514-00 | 0.43 | none | | yes | | TRAIL-induced apoptosis (1) | A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. | Kranz and Boutros | 2014 | 24442637 | Cell line | U251MG | Viability | Luminescence | SMART-pool siRNA | Genome-wide | siRNA | Z-score | > 4 | Author-submitted data | GR00240-S-2 | 378807 | XM_371237 | CATSPER4 | M-027514-00 | -0.73 | none | | no | Z-score -0.3295 | TRAIL-induced apoptosis (2) | A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. | Kranz and Boutros | 2014 | 24442637 | Cell line | U251MG | Viability (synthetic lethal) | Luminescence | SMART-pool siRNA | Genome-wide | siRNA | Differential score | > 3.6 AND viability Z-score < 4 | Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen. | GR00242-A-1 | 378807 | XM_371237 | CATSPER4 | np | np | none | | no | | Selective autophagy regulation (1) | Image-based genome-wide siRNA screen identifies selective autophagy factors. | Orvedahl et al. | 2011 | 22020285 | Cell line | HeLa/GFP-LC3 | Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression | Fluorescence | siGenome | Genome-wide | siRNA | Z-score | Complex criteria | | GR00247-A-1 | 378807 | | CATSPER4 | np | sp | none | | | rank: 3153 | Regulation of FOXO1 nuclear localization (1) | Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. | Senapedis et al. | 2011 | 21460183 | Cell line | U2OS | EGFP-FOXO1a protein expression and DNA content | Fluorescence | Human Genome library | Genome-wide | siRNA | Complex, sp | Complex criteria | | GR00249-S | 378807 | 378807 | CATSPER4 | J-027514-09 | -1.21328 | none | | no | number of cells compared to control (%): 84.96 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 378807 | 378807 | CATSPER4 | J-027514-09 | -1.07635 | none | | no | number of cells compared to control (%): 91.26 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 378807 | 378807 | CATSPER4 | M-027514-01 | 0.01225 | Decreased viability | | no | number of cells compared to control (%): 28.47 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 378807 | 378807 | CATSPER4 | s223972 | -2.353 | Decreased vaccinia virus (VACV) infection | | no | number of cells compared to control (%): 65.90 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 378807 | 378807 | CATSPER4 | s51801 | -0.40804 | none | | no | number of cells compared to control (%): 82.29 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 378807 | 378807 | CATSPER4 | s51802 | -0.34772 | none | | no | number of cells compared to control (%): 94.07 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00252-A-1 | 378807 | 378807 | CATSPER4 | np | -2.2352 | Decreased viability after Maraba virus infection | | | | Oncolytic Maraba rhabdovirus infection (1) | Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death. | Mahoney et al. | 2011 | 22014571 | Cell line | OVCAR-8 | Viability | Fluorescence | np | Genome-wide | siRNA | Composite MAD | np | All listed genes are final hits. Final hit if hit in > | GR00252-A-2 | 378807 | 378807 | CATSPER4 | np | -4.76026 | Decreased viability after Maraba virus infection | | | | Oncolytic Maraba rhabdovirus infection (2) | Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death. | Mahoney et al. | 2011 | 22014571 | Cell line | U373 | Viability | Fluorescence | np | Genome-wide | siRNA | Composite MAD | np | All listed genes are final hits. Final hit if hit in > | GR00252-A-3 | 378807 | 378807 | CATSPER4 | np | np | none | | | | Oncolytic Maraba rhabdovirus infection (3) | Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death. | Mahoney et al. | 2011 | 22014571 | Cell line | NCI-H226 | Viability | Fluorescence | np | Genome-wide | siRNA | Composite MAD | np | All listed genes are final hits. Final hit if hit in > | GR00255-A-1 | 378807 | 378807 | CATSPER4 | TRCN0000151820, TRCN0000153677, TRCN0000156924, TRCN0000157205, TRCN0000157305 | 0.69579843 | none | | | | Negative genetic interactions (1) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.0 | HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-2 | 378807 | 378807 | CATSPER4 | TRCN0000151820, TRCN0000153677, TRCN0000156924, TRCN0000157205, TRCN0000157305 | 0.542103658 | none | | | | Negative genetic interactions (2) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.0 | HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-3 | 378807 | 378807 | CATSPER4 | TRCN0000151820, TRCN0000153677, TRCN0000156924, TRCN0000157205, TRCN0000157305 | 0.135151131 | none | | | | Negative genetic interactions (3) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.2 | HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-4 | 378807 | 378807 | CATSPER4 | TRCN0000151820, TRCN0000153677, TRCN0000156924, TRCN0000157205, TRCN0000157305 | -0.290883408 | none | | | | Negative genetic interactions (4) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.2 | HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-5 | 378807 | 378807 | CATSPER4 | TRCN0000151820, TRCN0000153677, TRCN0000156924, TRCN0000157205, TRCN0000157305 | 0.644866466 | none | | | | Negative genetic interactions (5) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -0.8 | HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00293-A | 378807 | | CATSPER4 | np | 0.065 | none | | | | Combinatorial effect with paclitaxel | Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death | Sinnott et al. | 2014 | 24860162 | Cell line | HCC366 | Viability | Luminescence | Thermo-Fisher | Genome-wide | siRNA | Z-score | < -2.5 | Final hits according to the author are indicated in the comment. | GR00300-A | 378807 | | CATSPER4 | TRCN0000157205, TRCN0000151400, TRCN0000151422, TRCN0000153677, TRCN0000151820, TRCN0000156924, TRCN0000157595, TRCN0000154191, TRCN0000157305, TRCN0000151796 | 0 | none | | | | Combinatorial effect with RAF inhibitor PLX4720 | A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition. | Whittaker et al. | 2013 | 23288408 | Cell line | A375 | shRNA abundance | Sequencing | TRC | Genome-wide | shRNA | Number of shRNAs ranked Top1000 | > 2 | The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors. | GR00303-A | 378807 | XM_371237 | CATSPER4 | np | -0.04 | none | | | | Clear cell renal cell carcinoma (ccRCC) survival regulation | Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target | Gerlinger et al. | 2012 | 22362593 | Cell line | VHL-deficient RCC4 | Proliferation and Viability | Fluorescence | np | Genome-wide | siRNA | Z-score | < | In the phenotype data duplicates were in the original document, which have been removed. | GR00310-A-1 | 378807 | 378807 | CATSPER4 | np | 0.63 | none | | | | Sindbis virus (SINV) infection (1) | Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry | Ooi et al. | 2013 | 24367265 | Cell line | U2OS | Sindbis virus (SINV) reporter | Luminescence | Ambion Silencer V3 | Genome-wide | siRNA | Z-score | < -3 OR > 2 | | | 378807 | 378807 | CATSPER4 | np | 35.5 | none | | | 84,8% viability | | | | | | | | | | | | | | | | GR00313-A | 378807 | NM_198137 | CATSPER4 | np | -1.32 | none | | | | TNF-alpha pathway regulation | A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling. | Nickles et al. | 2012 | 22733992 | Cell line | HEK293T | NFkappaB pathway reporter | Luminescence | Qiagen | Genome-wide | siRNA | Z-score | < | Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%. | GR00360-A-1 | 378807 | ENSG00000188782 | CATSPER4 | 232436 | 0.815233333333333 | Mildly decreased CFP-tsO45G cell surface transport | | | | Secretory transport (1) | Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway | Simpson et al. | 2012 | 22660414 | Cell line | HeLa Kyoto | CFP-tsO45G cell surface transport | Fluorescence | Ambion | Genome-wide | siRNA | Deviation score | < - 1 OR > 0.75 (mild: 0.75 - 1.0; strong: > 1.0) | For validation screen see Secretory transport (2). | | 378807 | 378807 | CATSPER4 | | -0.03 | none | | | | | | | | | | | | | | | | | | | GR00376-A-1 | 378807 | 378807 | CATSPER4 | | 0.357387643 | none | | | | Mitigators of SS1P-induced immunotoxicity | Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity | Pasetto et al. | 2015 | 25713356 | Cell line | KB cells | Viability | Luminescence | Ambion Silencer Select Version 4 | Genome-wide | siRNA | RSA P-value | <0.001 | SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID | GR00376-A-2 | 378807 | 378807 | CATSPER4 | | 0.410357572 | none | | | | Sensitizers of SS1P-induced immunotoxicity | Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity | Pasetto et al. | 2015 | 25713356 | Cell line | KB cells | Viability | Luminescence | Ambion Silencer Select Version 4 | Genome-wide | siRNA | RSA P-value | <0.001 | SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID | GR00378-A | 378807 | | CATSPER4 | | 0.264378401 | none | | | | Poliovirus vaccine production | Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game | van der Sanden et al. | 2015 | 26581994 | Cell line | HEp-2C | Infection with Attenuated Poliovirus | ELISA | Dharmacon | Genome-wide | siRNA | Z-score | > | For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol | | 378807 | XM_033704 | CATSPER4 | | 53.931 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373876 | CATSPER4 | | 46.268 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_375713 | CATSPER4 | | 44.067 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_371326 | CATSPER4 | | 29.119 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378921 | CATSPER4 | | 25.522 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378832 | CATSPER4 | | 64.484 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373890 | CATSPER4 | | 38.948 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_371328 | CATSPER4 | | 24.534 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_377579 | CATSPER4 | | 20.252 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_377577 | CATSPER4 | | 33.953 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_371214 | CATSPER4 | | 48.5 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_372816 | CATSPER4 | | 25.518 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378917 | CATSPER4 | | 29.072 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_375845 | CATSPER4 | | 23.925 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_371330 | CATSPER4 | | 12.876 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_378828 | CATSPER4 | | 74.287 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_372775 | CATSPER4 | | 42.012 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_371350 | CATSPER4 | | 26.764 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_372805 | CATSPER4 | | 16.648 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_372807 | CATSPER4 | | 21.252 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_378908 | CATSPER4 | | 21.911 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_372774 | CATSPER4 | | 43.352 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373878 | CATSPER4 | | 47.523 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378852 | CATSPER4 | | 21.56 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_378848 | CATSPER4 | | 58.188 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378925 | CATSPER4 | | 43.962 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373824 | CATSPER4 | | 18.871 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_375849 | CATSPER4 | | 17.143 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_375821 | CATSPER4 | | 18.809 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_371237 | CATSPER4 | | 42.694 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373821 | CATSPER4 | | 30.55 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378914 | CATSPER4 | | 41.414 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_372755 | CATSPER4 | | 58.916 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378912 | CATSPER4 | | 34.058 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_375714 | CATSPER4 | | 47.877 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_375697 | CATSPER4 | | 68.716 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373822 | CATSPER4 | | 16.047 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_373831 | CATSPER4 | | 27.916 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373873 | CATSPER4 | | 17.646 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_371344 | CATSPER4 | | 28.397 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_375698 | CATSPER4 | | 16.736 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_371230 | CATSPER4 | | 36.786 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_377558 | CATSPER4 | | 30.191 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_373893 | CATSPER4 | | 26.145 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_377630 | CATSPER4 | | 22.345 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_371331 | CATSPER4 | | 44.391 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_378923 | CATSPER4 | | 50.23 | none | | | | | | | | | | | | | | | | | | | | 378807 | XM_372803 | CATSPER4 | | 18.896 | Decreased viability | | | | | | | | | | | | | | | | | | | | 378807 | XM_373837 | CATSPER4 | | 43.245 | none | | | | | | | | | | | | | | | | | | | GR00386-A-1 | 378807 | 378807 | CATSPER4 | | 94.3838815072079 | none | | | | NOD2 stimulation by MDP | A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. | Warner et al. | 2014 | 25170077 | Cell line | HEK293 stably expressing NOD2 | Viability | Luminescence | Dharmacon | Genome-wide | siRNA | Percentage growth | Decreased: <70, increased: >120 | Reagent IDs not provided | GR00386-A-2 | 378807 | 378807 | CATSPER4 | | -343.5 | Increased IL-8 secretion | | | | MDP-induced IL-8 secretion | A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. | Warner et al. | 2014 | 25170077 | Cell line | HEK293 stably expressing NOD2 | IL-8 secretion | ELISA | Dharmacon | Genome-wide | siRNA | Percent inhibition of IL-8 secretion | Increased: <-300, Decreased: >60 | Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided |