| GR00053-A | 58504 | NM_021226 | ARHGAP22 | np | sp | none | | no | | Genome stability | A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability. | Paulsen et al. | 2009 | 19647519 | Cell line | HeLa | gamma-H2AX phosphorylation and DNA content | Fluorescence | siARRAY human genome siRNA library | Genome-wide | siRNA | p-value | Complex criteria | Confidence groupings from 4 to 1 (highest level of confidence in group 4) | GR00054-A | 58504 | NM_021226 | ARHGAP22 | np | 0.945 | none | | no | | Combinatorial effect with paclitaxel | Synthetic lethal screen identification of chemosensitizer loci in cancer cells. | Whitehurst et al. | 2007 | 17429401 | Cell line | NCI-H1155 | Viability (synthetic lethal) | ATP level | # G-005000-01 | Genome-wide | siRNA | Paclitaxel/control ratio | Complex criteria | Additional information about 87 high-confidence hits | GR00056-A | 58504 | NM_021226 | ARHGAP22 | np | 1.119 | none | | no | | Melanogenesis | Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. | Ganesan et al. | 2008 | 19057677 | Cell line | MNT-1 | Melanin protein expression and viability | Absorbance and luminescence | rp | Genome-wide | siRNA | Normalized absorbance ratio | > 2 standard deviations below mean | Additional information about a secondary screen (retest to determine false-positive rate) | GR00057-A-1 | 58504 | NM_021226 | ARHGAP22 | M-019080-00 | 2.59 | none | | no | | Wnt/beta-catenin pathway regulation (1) | A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. | Tang et al. | 2008 | 18621708 | Cell line | HeLa | Wnt pathway reporter | Luminescence | Human siArray siRNA library | Genome-wide | siRNA | Z-score | > 4 | Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). | GR00057-A-2 | 58504 | NM_021226 | ARHGAP22 | M-019080-00 | sp | none | | no | | Wnt/beta-catenin pathway regulation (2) | A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. | Tang et al. | 2008 | 18621708 | Cell line | HeLa | Wnt pathway reporter | Luminescence | Human siArray siRNA library | Genome-wide | siRNA | Complex, SP | Complex criteria | Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). | GR00098-A-1 | 58504 | ENSG00000128805 | ARHGAP22 | ENSG00000128805 | sp | none | | no | | Cell division (1) | Genome-scale RNAi profiling of cell division in human tissue culture cells. | Kittler et al. | 2007 | 17994010 | Cell line | HeLa | Cell number and DNA content | Laser scanning cytometry | rp | Genome-wide | esiRNA | Complex, sp | Complex criteria | | GR00151-A-1 | 58504 | ENSG00000128805 | ARHGAP22 | np | -0.919 | none | | no | | Homologous recombination DNA double-strand break repair (HR-DSBR) (1) | A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. | Słabicki et al. | 2010 | 20613862 | Cell line | HeLa | (HR-DSBR) DR-GFP reporter | Flow cytometry | Custom-made | Genome-wide | esiRNA | Z-score | < -2 OR > 2 | | GR00180-A-1 | 58504 | 58504 | ARHGAP22 | PL-50063 | 0.925 | none | | no | | Hepatitis C virus replication (1) | A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. | Tai et al. | 2009 | 19286138 | Cell line | Huh7/Rep-Feo | HCV replicon RNA copy number | Luminescence | siARRAY Human Genome siRNA Library | Genome-wide | siRNA | q-value | Complex criteria | | GR00184-A-1 | 58504 | NM_021226 | ARHGAP22 | M-019080-00 | -1.87615142247699 | none | | no | | Self-renewal and pluripotency in human embryonic stem cells (1) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | POU5F1 protein expression | Fluorescence | SMARTpool siRNA library | Genome-wide | siRNA | Z-score | < -2 | | GR00196-A-1 | 58504 | ENSG00000128805 | | np | sp | none | | no | | TP53 interactions (1) | A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. | Krastev et al. | 2011 | 21642980 | Cell line | HCT116 ( wildtype and TP53 knockout) | TP53 protein expression and viability | Fluorescence | rp | Genome-wide | esiRNA | Complex, sp | Complex criteria | | GR00197-A-1 | 58504 | 58504 | ARHGAP22 | M-019080-00 | 2.792248028 | Increased HPV18 LCR reporter activity | | yes | deconvoluted validated siRNAs: 2 | Human papillomavirus oncogene expression regulation (1) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Genome-wide | siRNA | Z-score | > | Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5 | GR00197-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-02 | -1.919 | none | | yes | | Human papillomavirus oncogene expression regulation (3) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-03 | 0.216 | none | | yes | | Human papillomavirus oncogene expression regulation (3) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-01 | 3.031 | Increased HPV18 LCR reporter activity | | yes | | Human papillomavirus oncogene expression regulation (3) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-04 | 4.062 | Increased HPV18 LCR reporter activity | | yes | | Human papillomavirus oncogene expression regulation (3) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-4 | 58504 | 58504 | ARHGAP22 | D-019080-03 | 2.601 | Increased HPV18 LCR reporter activity | | yes | | Human papillomavirus oncogene expression regulation (4) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/16E2/18LCR clone 1 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-4 | 58504 | 58504 | ARHGAP22 | D-019080-02 | 2.873 | Increased HPV18 LCR reporter activity | | yes | | Human papillomavirus oncogene expression regulation (4) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/16E2/18LCR clone 1 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-4 | 58504 | 58504 | ARHGAP22 | D-019080-04 | 0.639 | none | | yes | | Human papillomavirus oncogene expression regulation (4) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/16E2/18LCR clone 1 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-4 | 58504 | 58504 | ARHGAP22 | D-019080-01 | 0.705 | none | | yes | | Human papillomavirus oncogene expression regulation (4) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/16E2/18LCR clone 1 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | np | Author-reviewed data | GR00197-A-5 | 58504 | 58504 | ARHGAP22 | D-019080-03 | 1.154 | none | | no | | Human papillomavirus oncogene expression regulation (5) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | BPV1 E2 protein expression | Fluorescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | < | Author-reviewed data | GR00197-A-5 | 58504 | 58504 | ARHGAP22 | D-019080-04 | 1.51 | none | | no | | Human papillomavirus oncogene expression regulation (5) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | BPV1 E2 protein expression | Fluorescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | < | Author-reviewed data | GR00197-A-5 | 58504 | 58504 | ARHGAP22 | D-019080-01 | 0.05 | none | | no | | Human papillomavirus oncogene expression regulation (5) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | BPV1 E2 protein expression | Fluorescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | < | Author-reviewed data | GR00197-A-5 | 58504 | 58504 | ARHGAP22 | D-019080-02 | 0.06 | none | | no | | Human papillomavirus oncogene expression regulation (5) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | BPV1 E2 protein expression | Fluorescence | Human siGENOME SMARTpool library | Selected genes | siRNA | Z-score | < | Author-reviewed data | GR00233-A-1 | 58504 | 58504 | ARHGAP22 | np | np | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection | | yes | siRNA pool validated | Hepatitis C virus (HCV) infection (1) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after virus infection | Fluorescence | Human Genome siARRAY siRNA G-005000–05 | Genome-wide | siRNA | Percentage of infected cells | < | | GR00233-A-2 | 58504 | 58504 | ARHGAP22 | D-019080-02 | 0.41 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection | | no | | Hepatitis C virus (HCV) infection (2) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after direct virus infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00233-A-2 | 58504 | 58504 | ARHGAP22 | D-019080-03 | 0.41 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection | | no | | Hepatitis C virus (HCV) infection (2) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after direct virus infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00233-A-2 | 58504 | 58504 | ARHGAP22 | D-019080-01 | 0.14 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection | | no | | Hepatitis C virus (HCV) infection (2) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after direct virus infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00233-A-2 | 58504 | 58504 | ARHGAP22 | D-019080-04 | 0.35 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after direct virus infection | | no | normalized cell number < 0.5 | Hepatitis C virus (HCV) infection (2) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after direct virus infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00233-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-02 | 0.36 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection | | no | | Hepatitis C virus (HCV) infection (3) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after viral supernatant infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00233-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-03 | 0.29 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection | | no | | Hepatitis C virus (HCV) infection (3) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after viral supernatant infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00233-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-01 | 0.24 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection | | no | | Hepatitis C virus (HCV) infection (3) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after viral supernatant infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00233-A-3 | 58504 | 58504 | ARHGAP22 | D-019080-04 | 0.22 | Decreased JFH-1 genotype 2a Hepatitis C virus (HCV) infection after viral supernatant infection | | no | normalized cell number in screen part one < 0.5 | Hepatitis C virus (HCV) infection (3) | A genome-wide genetic screen for host factors required for hepatitis C virus propagation. | Li et al. | 2009 | 19717417 | Cell line | Huh7.5.1 | HCV core protein expression and DNA content after viral supernatant infection | Fluorescence | siARRAY siRNA | Selected genes | siRNA | Normalized percentage of infected cells | < | | GR00236-A-1 | 58504 | 58504 | ARHGAP22 | M-019080-00 | 1.08330560314757 | none | | no | | Homologous recombination DNA double-strand break repair (HR-DSBR) (1) | A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. | Adamson et al. | 2012 | 22344029 | Cell line | DR-U2OS | (HR-DSBR) DR-GFP reporter and DNA content | Fluorescence | Human siGENOME siRNA (G-005000-05) | Genome-wide | siRNA | Relative HR ratio | < ~0.4 OR > 1.88 | Cutoff values correspond 2 standard deviations from the screen-wide mean | GR00240-S-1 | 58504 | NM_021226 | ARHGAP22 | M-019080-00 | 0.76 | none | | yes | | TRAIL-induced apoptosis (1) | A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. | Kranz and Boutros | 2014 | 24442637 | Cell line | U251MG | Viability | Luminescence | SMART-pool siRNA | Genome-wide | siRNA | Z-score | > 4 | Author-submitted data | GR00240-S-2 | 58504 | NM_021226 | ARHGAP22 | M-019080-00 | -0.07 | none | | no | Z-score 0.8585 | TRAIL-induced apoptosis (2) | A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. | Kranz and Boutros | 2014 | 24442637 | Cell line | U251MG | Viability (synthetic lethal) | Luminescence | SMART-pool siRNA | Genome-wide | siRNA | Differential score | > 3.6 AND viability Z-score < 4 | Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen. | GR00242-A-1 | 58504 | NM_021226 | ARHGAP22 | np | sp | none | | no | | Selective autophagy regulation (1) | Image-based genome-wide siRNA screen identifies selective autophagy factors. | Orvedahl et al. | 2011 | 22020285 | Cell line | HeLa/GFP-LC3 | Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression | Fluorescence | siGenome | Genome-wide | siRNA | Z-score | Complex criteria | | GR00247-A-1 | 58504 | | ARHGAP22 | np | sp | none | | | rank: 4637 | Regulation of FOXO1 nuclear localization (1) | Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. | Senapedis et al. | 2011 | 21460183 | Cell line | U2OS | EGFP-FOXO1a protein expression and DNA content | Fluorescence | Human Genome library | Genome-wide | siRNA | Complex, sp | Complex criteria | | GR00249-S | 58504 | 58504 | ARHGAP22 | J-019080-09 | 1.82092 | Increased vaccinia virus (VACV) infection | | no | number of cells compared to control (%): 72.17 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 58504 | 58504 | ARHGAP22 | M-019080-01 | 0.48239 | none | | no | number of cells compared to control (%): 50.81 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 58504 | 58504 | ARHGAP22 | s226939 | 0.00911 | none | | no | number of cells compared to control (%): 57.93 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 58504 | 58504 | ARHGAP22 | s33879 | 1.07985 | Increased vaccinia virus (VACV) infection | | no | number of cells compared to control (%): 92.99 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 58504 | 58504 | ARHGAP22 | s33880 | 0.23244 | none | | no | number of cells compared to control (%): 53.56 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00253-A | 58504 | NM_021226 | ARHGAP22 | np | -0.009 | none | | | | hepcidin regulation | Unbiased RNAi screen for hepcidin regulators links hepcidin suppression to proliferative Ras/RAF and nutrient-dependent mTOR signaling. | Mleczko-Sanecka et al. | 2014 | 24385536 | Cell line | Huh7 | hepcidin::fluc mRNA expression | Luminescence | siGenome siARRAY SMARTpool | Genome-wide | siRNA | Z-score | > | Cutoff < | GR00255-A-1 | 58504 | 58504 | ARHGAP22 | TRCN0000047203, TRCN0000047204, TRCN0000047205, TRCN0000047206 | 0.209528741 | none | | | | Negative genetic interactions (1) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.0 | HCT116 BLM-/- and HCT116 BLM+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-2 | 58504 | 58504 | ARHGAP22 | TRCN0000047203, TRCN0000047204, TRCN0000047205, TRCN0000047206 | -0.856120842 | none | | | | Negative genetic interactions (2) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.0 | HCT116 MUS81-/- and HCT116 MUS81+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-3 | 58504 | 58504 | ARHGAP22 | TRCN0000047203, TRCN0000047204, TRCN0000047205, TRCN0000047206 | 0.267066973 | none | | | | Negative genetic interactions (3) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.2 | HCT116 PTEN-/- and HCT116 PTEN+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-4 | 58504 | 58504 | ARHGAP22 | TRCN0000047203, TRCN0000047204, TRCN0000047205, TRCN0000047206 | -0.169032157 | none | | | | Negative genetic interactions (4) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -1.2 | HCT116 PTTG1-/- and HCT116 PTTG1+/+ cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00255-A-5 | 58504 | 58504 | ARHGAP22 | TRCN0000047203, TRCN0000047204, TRCN0000047205, TRCN0000047206 | 0.363327785 | none | | | | Negative genetic interactions (5) | A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities. | Vizeacoumar et al. | 2013 | 24104479 | Cell line | HCT116 | shRNA abundance | Microarray | np | Genome-wide | shRNA | differential Gene Activity Ranking Profile (dGARP) | < -0.8 | HCT116 KRASG13D/- and HCT116 KRAS+/- cells used. Cutoff corresponds to p-value < 0.05. Additional information about a secondary screen (genetic interactions with Cetuximab/Erbitux in LIM1215 cells) | GR00293-A | 58504 | | ARHGAP22 | np | 0.595 | none | | | | Combinatorial effect with paclitaxel | Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death | Sinnott et al. | 2014 | 24860162 | Cell line | HCC366 | Viability | Luminescence | Thermo-Fisher | Genome-wide | siRNA | Z-score | < -2.5 | Final hits according to the author are indicated in the comment. | GR00300-A | 58504 | | ARHGAP22 | TRCN0000047206, TRCN0000047204, TRCN0000047205, TRCN0000047203 | 0 | none | | | | Combinatorial effect with RAF inhibitor PLX4720 | A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition. | Whittaker et al. | 2013 | 23288408 | Cell line | A375 | shRNA abundance | Sequencing | TRC | Genome-wide | shRNA | Number of shRNAs ranked Top1000 | > 2 | The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors. | GR00303-A | 58504 | NM_021226 | ARHGAP22 | np | 0.73 | none | | | | Clear cell renal cell carcinoma (ccRCC) survival regulation | Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target | Gerlinger et al. | 2012 | 22362593 | Cell line | VHL-deficient RCC4 | Proliferation and Viability | Fluorescence | np | Genome-wide | siRNA | Z-score | < | In the phenotype data duplicates were in the original document, which have been removed. | GR00310-A-1 | 58504 | 58504 | ARHGAP22 | np | -0.16 | none | | | | Sindbis virus (SINV) infection (1) | Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry | Ooi et al. | 2013 | 24367265 | Cell line | U2OS | Sindbis virus (SINV) reporter | Luminescence | Ambion Silencer V3 | Genome-wide | siRNA | Z-score | < -3 OR > 2 | | | 58504 | 58504 | ARHGAP22 | np | 27.7 | none | | | 54,1% viability | | | | | | | | | | | | | | | | GR00313-A | 58504 | NM_021226 | ARHGAP22 | np | 0.38 | none | | | | TNF-alpha pathway regulation | A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling. | Nickles et al. | 2012 | 22733992 | Cell line | HEK293T | NFkappaB pathway reporter | Luminescence | Qiagen | Genome-wide | siRNA | Z-score | < | Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%. | GR00343-S | 58504 | 58504 | ARHGAP22 | TRCN0000047206 | 0.15250000000000163 | none | | | | Lentiviral shRNA "Achilles Heel" screen | Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia | Cole et al. | 2015 | 26058080 | Cell line | K562 | shRNA abundance | Fluorescence | Lentiviral shRNA library | Genome-wide | shRNA | Log2 ratio | < | Comparison of day 21 vs. day 0 abundance data | GR00343-S | 58504 | 58504 | ARHGAP22 | TRCN0000047203 | -1.3225000000000016 | shRNA abundance <= 50% | | | | Lentiviral shRNA "Achilles Heel" screen | Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia | Cole et al. | 2015 | 26058080 | Cell line | K562 | shRNA abundance | Fluorescence | Lentiviral shRNA library | Genome-wide | shRNA | Log2 ratio | < | Comparison of day 21 vs. day 0 abundance data | GR00343-S | 58504 | 58504 | ARHGAP22 | TRCN0000047204 | 0.5325000000000006 | none | | | | Lentiviral shRNA "Achilles Heel" screen | Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia | Cole et al. | 2015 | 26058080 | Cell line | K562 | shRNA abundance | Fluorescence | Lentiviral shRNA library | Genome-wide | shRNA | Log2 ratio | < | Comparison of day 21 vs. day 0 abundance data | GR00343-S | 58504 | 58504 | ARHGAP22 | TRCN0000047205 | 0.2774999999999981 | none | | | | Lentiviral shRNA "Achilles Heel" screen | Inhibition of the mitochondrial protease, ClpP, as a therapeutic strategy for human acute myeloid leukemia | Cole et al. | 2015 | 26058080 | Cell line | K562 | shRNA abundance | Fluorescence | Lentiviral shRNA library | Genome-wide | shRNA | Log2 ratio | < | Comparison of day 21 vs. day 0 abundance data | GR00355-A | 58504 | 58504 | ARHGAP22 | np | np | none | | | | PIP3-mediated epidermal growth factor (EGFR) endocytosis | PIP3 induces the recycling of receptor tyrosine kinases | Laketa et al. | 2014 | 24425787 | Cell line | HeLa Kyoto | Epidermal growth factor (EGFR) surface abundance | Fluorescence | Qiagen | Selected genes | siRNA | Deviation score | < -1 OR > 1 | HeLa Kyoto cells were stably transfected with EGFR-EYFP. Reagent Sequences (but no reagent ID) available in Table S4. | | 58504 | 58504 | ARHGAP22 | | -0.11000000000000001 | none | | | | | | | | | | | | | | | | | | | GR00376-A-1 | 58504 | 58504 | ARHGAP22 | | 0.667666068 | none | | | | Mitigators of SS1P-induced immunotoxicity | Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity | Pasetto et al. | 2015 | 25713356 | Cell line | KB cells | Viability | Luminescence | Ambion Silencer Select Version 4 | Genome-wide | siRNA | RSA P-value | <0.001 | SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID | GR00376-A-2 | 58504 | 58504 | ARHGAP22 | | 0.503676617 | none | | | | Sensitizers of SS1P-induced immunotoxicity | Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity | Pasetto et al. | 2015 | 25713356 | Cell line | KB cells | Viability | Luminescence | Ambion Silencer Select Version 4 | Genome-wide | siRNA | RSA P-value | <0.001 | SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID | GR00378-A | 58504 | | ARHGAP22 | | 2.743880012 | none | | | | Poliovirus vaccine production | Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game | van der Sanden et al. | 2015 | 26581994 | Cell line | HEp-2C | Infection with Attenuated Poliovirus | ELISA | Dharmacon | Genome-wide | siRNA | Z-score | > | For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol | | 58504 | NM_021226 | ARHGAP22 | | 32.536 | none | | | | | | | | | | | | | | | | | | | GR00386-A-1 | 58504 | 58504 | ARHGAP22 | | 56.8205693296602 | Decreased viability | | | | NOD2 stimulation by MDP | A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. | Warner et al. | 2014 | 25170077 | Cell line | HEK293 stably expressing NOD2 | Viability | Luminescence | Dharmacon | Genome-wide | siRNA | Percentage growth | Decreased: <70, increased: >120 | Reagent IDs not provided | GR00386-A-2 | 58504 | 58504 | ARHGAP22 | | -595.9 | Increased IL-8 secretion | | | | MDP-induced IL-8 secretion | A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. | Warner et al. | 2014 | 25170077 | Cell line | HEK293 stably expressing NOD2 | IL-8 secretion | ELISA | Dharmacon | Genome-wide | siRNA | Percent inhibition of IL-8 secretion | Increased: <-300, Decreased: >60 | Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided |