| GR00053-A | 9931 | XM_375485 | HELZ | np | sp | none | | no | | Genome stability | A genome-wide siRNA screen reveals diverse cellular processes and pathways that mediate genome stability. | Paulsen et al. | 2009 | 19647519 | Cell line | HeLa | gamma-H2AX phosphorylation and DNA content | Fluorescence | siARRAY human genome siRNA library | Genome-wide | siRNA | p-value | Complex criteria | Confidence groupings from 4 to 1 (highest level of confidence in group 4) | GR00054-A | 9931 | XM_375485 | HELZ | np | 0.85 | none | | no | | Combinatorial effect with paclitaxel | Synthetic lethal screen identification of chemosensitizer loci in cancer cells. | Whitehurst et al. | 2007 | 17429401 | Cell line | NCI-H1155 | Viability (synthetic lethal) | ATP level | # G-005000-01 | Genome-wide | siRNA | Paclitaxel/control ratio | Complex criteria | Additional information about 87 high-confidence hits | GR00056-A | 9931 | XM_375485 | HELZ | np | 0.997 | none | | no | | Melanogenesis | Genome-wide siRNA-based functional genomics of pigmentation identifies novel genes and pathways that impact melanogenesis in human cells. | Ganesan et al. | 2008 | 19057677 | Cell line | MNT-1 | Melanin protein expression and viability | Absorbance and luminescence | rp | Genome-wide | siRNA | Normalized absorbance ratio | > 2 standard deviations below mean | Additional information about a secondary screen (retest to determine false-positive rate) | GR00057-A-1 | 9931 | XM_375485 | HELZ | M-021180-00 | -1.16 | none | | no | | Wnt/beta-catenin pathway regulation (1) | A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. | Tang et al. | 2008 | 18621708 | Cell line | HeLa | Wnt pathway reporter | Luminescence | Human siArray siRNA library | Genome-wide | siRNA | Z-score | > 4 | Screen without Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). | GR00057-A-2 | 9931 | XM_375485 | HELZ | M-021180-00 | sp | none | | no | | Wnt/beta-catenin pathway regulation (2) | A genome-wide RNAi screen for Wnt/beta-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer. | Tang et al. | 2008 | 18621708 | Cell line | HeLa | Wnt pathway reporter | Luminescence | Human siArray siRNA library | Genome-wide | siRNA | Complex, SP | Complex criteria | Screen with Wnt3A stimulation. Additional information about secondary screens (Dharmacon and Qiagen libraries). | GR00098-A-1 | 9931 | ENSG00000198265 | HELZ | ENSG00000198265 | sp | none | | no | | Cell division (1) | Genome-scale RNAi profiling of cell division in human tissue culture cells. | Kittler et al. | 2007 | 17994010 | Cell line | HeLa | Cell number and DNA content | Laser scanning cytometry | rp | Genome-wide | esiRNA | Complex, sp | Complex criteria | | GR00149-A-1 | 9931 | 9931 | HELZ | 22101 | -8.08 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 9931 | 9931 | HELZ | 22101 | -28.51 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 9931 | 9931 | HELZ | 22197 | 5.08 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 9931 | 9931 | HELZ | 22197 | -19.61 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 9931 | 9931 | HELZ | 22293 | -19.79 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 9931 | 9931 | HELZ | 22293 | -13.49 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 9931 | 9931 | HELZ | 214035 | -40.68 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00149-A-1 | 9931 | 9931 | HELZ | 214035 | -26.8 | none | | no | | Ciliogenesis and cilium length (1) | Functional genomic screen for modulators of ciliogenesis and cilium length. | Kim et al. | 2010 | 20393563 | Cell line | htRPE | Smoothed protein expression | Fluorescence | Human druggable genome siRNA library V3.1 | Druggable genes | siRNA | Normalized percent inhibition | > 1.5 OR < -1.5 standard deviations from mean | | GR00151-A-1 | 9931 | ENSG00000198265 | HELZ | np | 0.583 | none | | no | | Homologous recombination DNA double-strand break repair (HR-DSBR) (1) | A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. | Słabicki et al. | 2010 | 20613862 | Cell line | HeLa | (HR-DSBR) DR-GFP reporter | Flow cytometry | Custom-made | Genome-wide | esiRNA | Z-score | < -2 OR > 2 | | GR00180-A-1 | 9931 | 9931 | HELZ | PL-50053 | 0.899 | none | | no | | Hepatitis C virus replication (1) | A functional genomic screen identifies cellular cofactors of hepatitis C virus replication. | Tai et al. | 2009 | 19286138 | Cell line | Huh7/Rep-Feo | HCV replicon RNA copy number | Luminescence | siARRAY Human Genome siRNA Library | Genome-wide | siRNA | q-value | Complex criteria | | GR00184-A-1 | 9931 | XM_375485 | HELZ | M-021180-00 | -2.90682892702874 | Decreased POU5F1-GFP protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (1) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | POU5F1 protein expression | Fluorescence | SMARTpool siRNA library | Genome-wide | siRNA | Z-score | < -2 | | GR00184-A-2 | 9931 | NM_014877 | HELZ | D-021180-01 | 0.497066818537451 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (2) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-2 | 9931 | NM_014877 | HELZ | D-021180-02 | 0.260544617131811 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (2) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-2 | 9931 | NM_014877 | HELZ | D-021180-03 | 0.516508353260936 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (2) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-2 | 9931 | NM_014877 | HELZ | D-021180-04 | 0.3960465152245 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (2) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-3 | 9931 | NM_014877 | HELZ | D-021180-01 | 0.520705528298751 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (3) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-3 | 9931 | NM_014877 | HELZ | D-021180-03 | 0.389447944990094 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (3) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-3 | 9931 | NM_014877 | HELZ | D-021180-04 | 0.441440518076921 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (3) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-3 | 9931 | NM_014877 | HELZ | D-021180-02 | 0.487878700160777 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (3) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-4 | 9931 | NM_014877 | HELZ | D-021180-01 | 0.335229938041353 | Decreased POU5F1-GFP protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (4) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | POU5F1 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-4 | 9931 | NM_014877 | HELZ | D-021180-02 | 0.34298687318456 | Decreased POU5F1-GFP protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (4) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | POU5F1 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-4 | 9931 | NM_014877 | HELZ | D-021180-03 | 0.314897305722394 | Decreased POU5F1-GFP protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (4) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | POU5F1 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-4 | 9931 | NM_014877 | HELZ | D-021180-04 | 0.328660609021207 | Decreased POU5F1-GFP protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (4) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | hESC H1 | POU5F1 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-5 | 9931 | NM_014877 | HELZ | D-021180-04 | 0.904593058400616 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (5) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-5 | 9931 | NM_014877 | HELZ | D-021180-01 | 0.775727641602168 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (5) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-5 | 9931 | NM_014877 | HELZ | D-021180-02 | 0.44661453945378 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (5) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-5 | 9931 | NM_014877 | HELZ | D-021180-03 | 0.687829194019432 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (5) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-6 | 9931 | NM_014877 | HELZ | D-021180-03 | 0.685571195473686 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (6) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-6 | 9931 | NM_014877 | HELZ | D-021180-01 | 0.908314886802685 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (6) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-6 | 9931 | NM_014877 | HELZ | D-021180-04 | 0.843181100445838 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (6) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-6 | 9931 | NM_014877 | HELZ | D-021180-02 | 0.621209874179877 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (6) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES2 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-7 | 9931 | NM_014877 | HELZ | D-021180-01 | 0.664708867307901 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (7) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-7 | 9931 | NM_014877 | HELZ | D-021180-02 | 0.353467586095618 | Decreased OCT4 protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (7) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-7 | 9931 | NM_014877 | HELZ | D-021180-03 | 1.02337816445339 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (7) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-7 | 9931 | NM_014877 | HELZ | D-021180-04 | 0.869847739617229 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (7) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | OCT4 protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-8 | 9931 | NM_014877 | HELZ | D-021180-04 | 0.805572383143248 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (8) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-8 | 9931 | NM_014877 | HELZ | D-021180-03 | 0.847328411979201 | none | | yes | | Self-renewal and pluripotency in human embryonic stem cells (8) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-8 | 9931 | NM_014877 | HELZ | D-021180-01 | 0.701765753230993 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (8) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00184-A-8 | 9931 | NM_014877 | HELZ | D-021180-02 | 0.463297040679224 | Decreased NANOG protein expression | | yes | | Self-renewal and pluripotency in human embryonic stem cells (8) | A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. | Chia et al. | 2010 | 20953172 | Cell line | HES3 | NANOG protein expression | Fluorescence | siGENOME siRNA library | Selected genes | siRNA | Relative expression | < 0.75 | | GR00196-A-1 | 9931 | ENSG00000198265 | | np | sp | none | | no | | TP53 interactions (1) | A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. | Krastev et al. | 2011 | 21642980 | Cell line | HCT116 ( wildtype and TP53 knockout) | TP53 protein expression and viability | Fluorescence | rp | Genome-wide | esiRNA | Complex, sp | Complex criteria | | GR00197-A-1 | 9931 | 9931 | HELZ | M-021180-00 | 0.701144234 | none | | no | | Human papillomavirus oncogene expression regulation (1) | Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression. | Smith et al. | 2010 | 20133580 | Cell line | C33A/BE2/18LCR c4 | HPV18 LCR reporter activity | Luminescence | Human siGENOME SMARTpool library | Genome-wide | siRNA | Z-score | > | Author-submitted data. Phenotype strength according to Z-scores: weak: 2 - 3; moderate: 3 - 5; strong: > 5 | GR00232-A-1 | 9931 | NM_014877 | HELZ | v2HS_95826 | 1.00673116766667 | Decreased ionizing radiation sensitivity | | no | | DNA damage regulation after ionizing radiation (1) | A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. | Hurov et al. | 2010 | 20810650 | Cell line | U2OS | Ionizing radiation sensitivity | Fluorescence | pSMP | Genome-wide | shRNA | log2 mean ratio | < -1 OR > 1 | The shRNA library is in a MSCV-PURO-based vector (MSCV-PM; Open Biosystems nomenclature is pSMP). The shRNAs with the “V3LHS” identifier were subcloned into the MSCV-PM vector from the pGIPZ vector. Additional information about a secondary screen in H460 cells. | GR00236-A-1 | 9931 | 9931 | HELZ | M-021180-00 | 1.31081095501057 | none | | no | | Homologous recombination DNA double-strand break repair (HR-DSBR) (1) | A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. | Adamson et al. | 2012 | 22344029 | Cell line | DR-U2OS | (HR-DSBR) DR-GFP reporter and DNA content | Fluorescence | Human siGENOME siRNA (G-005000-05) | Genome-wide | siRNA | Relative HR ratio | < ~0.4 OR > 1.88 | Cutoff values correspond 2 standard deviations from the screen-wide mean | GR00240-S-1 | 9931 | XM_375485 | HELZ | M-021180-00 | -0.1 | none | | yes | | TRAIL-induced apoptosis (1) | A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. | Kranz and Boutros | 2014 | 24442637 | Cell line | U251MG | Viability | Luminescence | SMART-pool siRNA | Genome-wide | siRNA | Z-score | > 4 | Author-submitted data | GR00240-S-2 | 9931 | XM_375485 | HELZ | M-021180-00 | -0.75 | none | | no | Z-score -0.766 | TRAIL-induced apoptosis (2) | A synthetic lethal screen identifies FAT1 as an antagonist of caspase-8 in extrinsic apoptosis. | Kranz and Boutros | 2014 | 24442637 | Cell line | U251MG | Viability (synthetic lethal) | Luminescence | SMART-pool siRNA | Genome-wide | siRNA | Differential score | > 3.6 AND viability Z-score < 4 | Author-submitted data. Z-scores from viability screen (1) are considered in score interpretation for this screen. | GR00242-A-1 | 9931 | XM_375485 | HELZ | np | sp | none | | no | | Selective autophagy regulation (1) | Image-based genome-wide siRNA screen identifies selective autophagy factors. | Orvedahl et al. | 2011 | 22020285 | Cell line | HeLa/GFP-LC3 | Sindbis virus (SIN) capsid SIN-mCherry.capsid and autophagosome GFP–LC3 protein expression | Fluorescence | siGenome | Genome-wide | siRNA | Z-score | Complex criteria | | GR00247-A-1 | 9931 | | HELZ | np | sp | none | | | rank: 3109 | Regulation of FOXO1 nuclear localization (1) | Whole genome siRNA cell-based screen links mitochondria to Akt signaling network through uncoupling of electron transport chain. | Senapedis et al. | 2011 | 21460183 | Cell line | U2OS | EGFP-FOXO1a protein expression and DNA content | Fluorescence | Human Genome library | Genome-wide | siRNA | Complex, sp | Complex criteria | | GR00249-S | 9931 | 9931 | HELZ | J-021180-09 | -0.10307 | none | | no | number of cells compared to control (%): 89.48 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 9931 | 9931 | HELZ | M-021180-01 | -1.45199 | none | | no | number of cells compared to control (%): 53.32 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 9931 | 9931 | HELZ | s19269 | 1.21534 | Increased vaccinia virus (VACV) infection | | no | number of cells compared to control (%): 100.37 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 9931 | 9931 | HELZ | s19270 | -1.05662 | none | | no | number of cells compared to control (%): 90.52 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00249-S | 9931 | 9931 | HELZ | s19271 | -1.799 | Decreased vaccinia virus (VACV) infection | | no | number of cells compared to control (%): 53.98 | Vaccinia virus (VACV) infection | Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis. | Sivan et al. | 2013 | 23401514 | Cell line | HeLa | Vaccinia virus VACV IHD-J/GFP protein expression and DNA content | Fluorescence | Silencer Select Version 4, siGENOME SMARTpool and OnTargetPlus | Genome-wide | siRNA | Z-score | > | Author-submitted data. Primary screen. Decreased viability phenotype if number of cells compared to control < 50 %. | GR00293-A | 9931 | | HELZ | np | -1.116 | none | | | | Combinatorial effect with paclitaxel | Mechanisms Promoting Escape from Mitotic Stress−Induced Tumor Cell Death | Sinnott et al. | 2014 | 24860162 | Cell line | HCC366 | Viability | Luminescence | Thermo-Fisher | Genome-wide | siRNA | Z-score | < -2.5 | Final hits according to the author are indicated in the comment. | GR00300-A | 9931 | | HELZ | TRCN0000162533, TRCN0000162269, TRCN0000160132, TRCN0000159972, TRCN0000158502 | 0 | none | | | | Combinatorial effect with RAF inhibitor PLX4720 | A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition. | Whittaker et al. | 2013 | 23288408 | Cell line | A375 | shRNA abundance | Sequencing | TRC | Genome-wide | shRNA | Number of shRNAs ranked Top1000 | > 2 | The A375 cell line used here harbours the BRAF V600E mutation and is therefore sensitive to RAF inhibitors. | GR00303-A | 9931 | XM_375485 | HELZ | np | 0.08 | none | | | | Clear cell renal cell carcinoma (ccRCC) survival regulation | Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target | Gerlinger et al. | 2012 | 22362593 | Cell line | VHL-deficient RCC4 | Proliferation and Viability | Fluorescence | np | Genome-wide | siRNA | Z-score | < | In the phenotype data duplicates were in the original document, which have been removed. | GR00306-A | 9931 | 9931 | HELZ | np | 48.07 | Decreased human papilloma virus 16 (HPV16) pseudovirus infection | | | | Human papillomavirus 16 (HPV16) infection | Genome-wide siRNA screen identifies the retromer as a cellular entry factor for human papillomavirus | Lipovsky et al. | 2013 | 23569269 | Cell line | HeLa-S3 | HPV16 pseudovirus reporter | Fluorescence | Dharmacon SMARTpool | Genome-wide | siRNA | Modified activity score (MAS1) | Top 1000 | | GR00310-A-1 | 9931 | 9931 | HELZ | np | 0.14 | none | | | | Sindbis virus (SINV) infection (1) | Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry | Ooi et al. | 2013 | 24367265 | Cell line | U2OS | Sindbis virus (SINV) reporter | Luminescence | Ambion Silencer V3 | Genome-wide | siRNA | Z-score | < -3 OR > 2 | | | 9931 | 9931 | HELZ | np | -4 | none | | | 82,3% viability | | | | | | | | | | | | | | | | GR00313-A | 9931 | NM_014877 | HELZ | np | 0.4 | none | | | | TNF-alpha pathway regulation | A Genome-Wide RNA Interference Screen Identifies Caspase 4 as a Factor Required for Tumor Necrosis Factor Alpha Signaling. | Nickles et al. | 2012 | 22733992 | Cell line | HEK293T | NFkappaB pathway reporter | Luminescence | Qiagen | Genome-wide | siRNA | Z-score | < | Additional filters were a reduction in firefly luciferase levels by at least 50% compared to the mean of the experiment and a concomitant reduction of renilla luciferase expression of not more than 30%. | GR00368-A | 9931 | 9931 | HELZ | | 0.78 | Decreased viability ratio | | | wt viability: 0,91 | Combinatorial effect with E-Cadherin | Synthetic Lethal Screens Identify Vulnerabilities in GPCR Signaling and Cytoskeletal Organization in E-Cadherin–Deficient Cells | Telford et al. | 2015 | 25777964 | Cell line | MCF10A | Viability (synthetic lethal) | Luminescence | Dharmacon | Genome-wide | siRNA | CDH1(-/-)/wt viability ratio | < | MCF10A wild type viability higher-or-equal to 50% was considered as additional cutoff (values noted in "comment" section). | GR00371-A-1 | 9931 | 9931 | HELZ | | 1.18507549222 | Increased Nanog expression | | | Ambion | Nanog expression in absence of bFGF and TGFbeta | Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways | Gonzales et al. | 2015 | 26232226 | Cell line | NANOG-GFP H1 hESC | NANOG expression | Fluorescence | Dharmacon and Ambion | Selected genes | siRNA | Z-score | >1,25 OR >1,5 [in at least two replicates] | Z-score shown is an average of the z-scores from the three replicates performed for each gene. In the "Comment" field, the siRNA library used for each particular gene is noted. | GR00371-A-2 | 9931 | HELZ | 9931 | | 0.045125991142 | none | | | Ambion | Nanog expression in presence of TGFbeta inhibitor | Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways | Gonzales et al. | 2015 | 26232226 | Cell line | NANOG-GFP H1 hESC | NANOG expression | Fluorescence | Dharmacon and Ambion | Selected genes | siRNA | Z-score | >1,25 OR >1,5 [in at least two replicates] | Z-score shown is an average of the z-scores from the three replicates performed for each gene. In the "Comment" field, the siRNA library used for each particular gene is noted. | GR00371-A-3 | 9931 | 9931 | HELZ | | 0.446564982087 | none | | | Ambion | Nanog expression in presence of MEK inhibitor | Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways | Gonzales et al. | 2015 | 26232226 | Cell line | NANOG-GFP H1 hESC | NANOG expression | Fluorescence | Dharmacon and Ambion | Selected genes | siRNA | Z-score | >1,25 OR >1,5 [in at least two replicates] | Z-score shown is an average of the z-scores from the three replicates performed for each gene. In the "Comment" field, the siRNA library used for each particular gene is noted. | GR00371-A-4 | 9931 | 9931 | HELZ | | 0.531202894375 | none | | | Ambion | Nanog expression in presence of PI3K inhibitor | Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways | Gonzales et al. | 2015 | 26232226 | Cell line | NANOG-GFP H1 hESC | NANOG expression | Fluorescence | Dharmacon and Ambion | Selected genes | siRNA | Z-score | >1,25 OR >1,5 [in at least two replicates] | Z-score shown is an average of the z-scores from the three replicates performed for each gene. In the "Comment" field, the siRNA library used for each particular gene is noted. | GR00371-A-5 | 9931 | 9931 | HELZ | | -0.591285718469 | none | | | Ambion | Nanog expression in presence of retinoic acid | Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways | Gonzales et al. | 2015 | 26232226 | Cell line | NANOG-GFP H1 hESC | NANOG expression | Fluorescence | Dharmacon and Ambion | Selected genes | siRNA | Z-score | >1,25 OR >1,5 [in at least two replicates] | Z-score shown is an average of the z-scores from the three replicates performed for each gene. In the "Comment" field, the siRNA library used for each particular gene is noted. | GR00376-A-1 | 9931 | 9931 | HELZ | | 0.035266245 | none | | | | Mitigators of SS1P-induced immunotoxicity | Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity | Pasetto et al. | 2015 | 25713356 | Cell line | KB cells | Viability | Luminescence | Ambion Silencer Select Version 4 | Genome-wide | siRNA | RSA P-value | <0.001 | SS1P was applied in a "high dose", ≈EC90, 13 ng/ml. Cutoff was derived from data submitted to Pubchem (ID 1117281). Reagent sequences but no ID | GR00376-A-2 | 9931 | 9931 | HELZ | | 0.368506818 | none | | | | Sensitizers of SS1P-induced immunotoxicity | Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity | Pasetto et al. | 2015 | 25713356 | Cell line | KB cells | Viability | Luminescence | Ambion Silencer Select Version 4 | Genome-wide | siRNA | RSA P-value | <0.001 | SS1P was applied in a "low dose", ≈EC30, 3 ng/ml. Cutoff was derived from data submitted to PubChem (ID 1117281). Reagent sequences but no ID | GR00378-A | 9931 | | HELZ | | -1.269754068 | none | | | | Poliovirus vaccine production | Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game | van der Sanden et al. | 2015 | 26581994 | Cell line | HEp-2C | Infection with Attenuated Poliovirus | ELISA | Dharmacon | Genome-wide | siRNA | Z-score | > | For infection, a single lot of the attenuated Sabin type 2 poliovirus was used for the screen. Gene IDs were not provided, only the gene name/symbol | | 9931 | XM_375485 | HELZ | | 53.848 | none | | | | | | | | | | | | | | | | | | | GR00386-A-1 | 9931 | 9931 | HELZ | | 71.5868681197148 | none | | | | NOD2 stimulation by MDP | A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. | Warner et al. | 2014 | 25170077 | Cell line | HEK293 stably expressing NOD2 | Viability | Luminescence | Dharmacon | Genome-wide | siRNA | Percentage growth | Decreased: <70, increased: >120 | Reagent IDs not provided | GR00386-A-2 | 9931 | 9931 | HELZ | | 85.1 | Decreased IL-8 secretion | | | | MDP-induced IL-8 secretion | A genome-wide small interfering RNA (siRNA) screen reveals nuclear factor-κB (NF-κB)-independent regulators of NOD2-induced interleukin-8 (IL-8) secretion. | Warner et al. | 2014 | 25170077 | Cell line | HEK293 stably expressing NOD2 | IL-8 secretion | ELISA | Dharmacon | Genome-wide | siRNA | Percent inhibition of IL-8 secretion | Increased: <-300, Decreased: >60 | Concentration of IL-8 was measured from cell supernatants by sandwich ELISA. IL-8 values (pg/ml) were normalized to IL-8 secreted in cells treated with RIPK2-specific siRNA (100% inhibition) and non-targeting siRNA (0% inhibition). Secondary validating screen assessed 554 genes whose silencing affected MDP-induced IL-8 secretion in the primary screen. Final validated IL-8 regulators (positive or negative) are listed in the comments column. Reagent IDs not provided |