| 171990 | O01761 | UNC89_CAEEL | Muscle M-line assembly protein unc-89 (Inactive serine/threonine-protein kinase unc-89) (Obscurin) (Uncoordinated protein 89) | DISRUPTION PHENOTYPE | Mutants lacking isoform a, isoform b, isoform e and isoform f have an abnormal organization of the myofilament lattice of body wall and pharyngeal muscles (PubMed:16453163, PubMed:18801371, PubMed:22621901, PubMed:27009202). In body wall muscles, unc-15/paramyosin accumulates in large foci outside thick filaments and myosin heavy chains unc-54 and myo-3 fail to assemble into parallel myofibrils (PubMed:27009202). In addition, myosin thick filaments are disorganized with the formation of abnormal myosin heavy chain myo-3 aggregates and V-shaped crossing of A bands (PubMed:18801371, PubMed:22621901). In mutants lacking isoform b, isoform c, isoform d and isoform f, myo-3 fails to assemble into parallel myofibrils whereas unc-54 and unc-15 localization is normal (PubMed:27009202). Mutants lacking isoform b, isoform c, isoform d and isoform f have defects only in body wall muscle structure (PubMed:16453163). RNAi-mediated knockdown of isoform a or isoform b, isoform c and isoform d causes similar defects in the organization although RNAi-mediated knockdown of isoform c causes a less severe defect in myofilament organization (PubMed:15313609). In mutants lacking isoform a, isoform b, isoform e and isoform f, mei-1 protein levels are decreased by 20 percent (PubMed:22621901). RNAi-mediated knockdown of isoform a, isoform b, isoform e and isoform f but not of isoforms c and d disrupts sca-1 localization to linear punctate structures along in the M line in body wall muscles (PubMed:22768340). RNAi-mediated knockdown has no effect on ovulation (PubMed:17326220). {ECO:0000269|PubMed:15313609, ECO:0000269|PubMed:16453163, ECO:0000269|PubMed:17326220, ECO:0000269|PubMed:18801371, ECO:0000269|PubMed:22621901, ECO:0000269|PubMed:22768340, ECO:0000269|PubMed:27009202}. |