| 173410 | Q95XZ5 | NIPBL_CAEEL | Nipped-B-like protein scc-2 (Prion-like-(Q/N-rich) domain-bearing protein 85) (SCC2 homolog) | DISRUPTION PHENOTYPE | RNAi-mediated knockdown results in 100% embryonic lethality (PubMed:15146185, PubMed:16802858). Any survivors display a paralyzed uncoordinated phenotype, body morphology defects and sometimes a vulval defect (PubMed:15146185). RNAi-mediated knockdown results in chromosome segregation defects in early embryos with lagging chromosomes at the anaphase phase of mitosis (PubMed:16802858). RNAi-mediated knockdown results in cytological defects in the pachytene and diakinesis phases of meiosis in the germline (PubMed:21856158). RNAi-mediated knockdown abolishes loading of cohesin complex components smc-1, smc-3 and rec-8 onto the to the axial element of meiotic chromosomes in the germline, however these subunits do accumulate in the mitotic nuclei and the meiotic S phase nuclei that precede the start of meiotic prophase (PubMed:21856158). RNAi-mediated knockdown results in defective DNA double-strand break repair during meiosis resulting in an accumulation of rad-51-positive recombination intermediates and elongated rad-51-positive recombination structures in the mid and late pachytene region of the germline (PubMed:21856158). There is an increase in apoptosis in response to the accumulation of recombination intermediates, but only in the presence of smc-1, indicative of a defective DNA damage response (PubMed:21856158). {ECO:0000269|PubMed:15146185, ECO:0000269|PubMed:16802858, ECO:0000269|PubMed:21856158}. |