| 3565068 | G5EDB2 | MADD3_CAEEL | Probable dual specificity protein kinase madd-3 (EC 2.7.11.-) (Muscle arm development defective protein 3) | DISRUPTION PHENOTYPE | Viable, but sterile. Defective extension of body wall muscle connections or arms towards the ventral nerve cord. Reduced expression of the late endosome marker rab-7, and the eva-1 and unc-40 receptors, which are expressed in muscles, and impaired recruitment of madd-4 to the muscle membrane. Double knockout with cup-5 results in increased expression of the eva-1 receptor and rab-7 positive endosomes. Double knockout with eva-1, gex-2, madd-2, madd-4, mkk-4, unc-15, unc-60, unc-93 or unc-98 results in severe muscle arm extension defects as compared to the single knockouts. Double knockout with proteins involved in the p38 MAPK signaling pathway including cebp-1, mak-2, pmk-3 or sek-3 suppress the muscle arm extension defects and eva-1 expression defects in the madd-3 single knockout. Double knockout with dlk-1 also suppresses the eva-1 expression defect, but does not suppress the muscle arm extension defects in the madd-3 single knockout. Double knockout with cebp-1, mak-2 or pmk-3 restores the defect in the recruitment of madd-4 to the muscle membrane in the madd-3 single knockout. Furthermore, double knockout with pmk-3 restores the reduced rab-7 expression level defect in the madd-3 single knockout. Double knockout with unc-54 results in lethality. Triple knockout with unc-54, and either cebp-1, dlk-1, mak-2, pmk-3 or sek-3 results in paralysis (as in the unc-54 single knockout), and suppresses the lethality phenotype in the double madd-3 and unc-54 mutant. {ECO:0000269|PubMed:27123983}. |