| 3346201 | A8DYP0 | OBSCN_DROME | Obscurin (EC 2.7.11.1) (Muscle M-line assembly protein Unc-89) | DISRUPTION PHENOTYPE | RNAi-mediated knockdown either in the indirect flight muscles (IFM) or in all muscles prevents flies from flying without affecting jumping and slightly reduces larval crawling (PubMed:22467859). Causes severe defects in IFM sarcomere development and organization resulting in narrower myofibrils (PubMed:22467859). During sarcomere development at the pupal stage, myosin is mislocalized and no regular sarcomeres are formed (PubMed:22467859). In the adult, abnormal localization of myosin in the mid-region of the sarcomere, H zone shifts from the middle of the sarcomere and M lines are less defined (PubMed:22467859). Thick filaments are asymmetric with the bare zone often shifted towards the end of the filament (PubMed:22467859). Thick filament length is mostly normal although some are longer and by-pass the Z line (PubMed:22467859). Thin filaments are abnormally shorter or longer and sometimes extend into the H zone (PubMed:22467859). Several Z and M lines associated proteins are mislocalized (PubMed:22467859, PubMed:26251439). zormin is more diffused in both Z and M lines and tropomodulin is confined to the core of the myofibril in the H zone (PubMed:22467859). ball is still partially localized to the Z line but appears more diffuse across the sarcomere compare to wild type (PubMed:26251439). mask localization to Z lines is not affected but is absent or in a punctate form in the M line (PubMed:26251439). kettin localization to the Z line is not affected (PubMed:22467859). {ECO:0000269|PubMed:22467859, ECO:0000269|PubMed:26251439}. |